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查看更多产品信息 GeneAmp® PCR System 9700, 96-Well Silver - FAQs (N8050001)
18 个常见问题解答
热循环仪的热盖经过设计可保证对微孔板施加合适的压力，以获得高效的PCR反应。使用PCR反应管时，热盖对反应管的压力过大可导致反应管变形。为避免出现这种情况，我们建议使用下述托盘/固定装置：
•货号4381850，用于Veriti系统的MicroAmp 96孔托盘/固定装置——这一托盘适用于MicroAmp联管（或MicroAmp无盖反应管，0.2 mL），和Veriti热循环仪、SimpliAmp热循环仪和Proflex PCR系统兼容。
•货号403081，MicroAmp 96孔托盘/固定装置——这一托盘适用于MicroAmp联管（或MicroAmp无盖反应管，0.2 mL），可用于7000系统、2720热循环仪和GeneAmp 9700型PCR系统。
•货号4379983，用于VeriFlex模块的MicroAmp 96孔托盘——这一托盘适用于MicroAmp联管（或MicroAmp带盖反应管，0.1 mL），可用于Veriti热循环仪、Veriti快速热循环仪、SimpliAmp热循环仪和Proflex PCR系统。
点击 这里(https://tools.thermofisher.com/content/sfs/brochures/cms_042718.pdf)，可查看GeneAmp 9700型PCR系统的丢失校准文件替换指南。
请参考GeneAmp 9700 PCR系统及其模块类型专用的 实验方案指南(https://tools.thermofisher.com/content/sfs/manuals/cms_070449.pdf) 。
如需设置永久恒定温度，请将时间设置为“99.59”。屏幕将在此温度处默认显示（∞）图标。
没有，GeneAmp 9700 PCR系统未整合梯度控温功能。我们的新款热循环仪 - ProFlex PCR 系统 与 Veriti 热循环仪 - 拥有超越梯度控温技术的VeriFlex模块。
Auto Delta特性能够帮助您在循环阶段中逐渐增高或降低每一循环某一步骤的温度。其中一个常见应用就是降落PCR（touchdown PCR）。
请参见下面不同模块类型的最大模块变温速率（°C/秒）：
-96孔铝制模块： 2.3
-96孔金制模块： 2.6
-96孔银制模块： 2.6
-双96孔： 1.6
-双384孔： 1.8
-60孔： 1.9
孔板与塑料耗材均为特定模块类型所专用。请参考此表(https://www.thermofisher.com/cn/zh/home/life-science/pcr/pcr-plastics.html)，了解您的模块类型兼容的塑料耗材。
GeneAmp 9700 PCR系统提供多种反应孔形式和材质的可互换样本模块，适用于各类应用。
96孔铝制模块
96孔金制模块
96孔银制模块
双96孔
双384孔
60孔
The heated thermal cycler lid is designed to make sure that optimized pressure is applied on the microplate for an efficient PCR reaction. When using PCR tubes, excess pressure on the tubes, from the heated lid can cause deformation of the tubes. To avoid this, we recommend using the Tray/Retainer set, as shown below:
Cat. No. 4381850, MicroAmp 96-Well Tray/Retainer Set for Veriti Systems – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the Veriti Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.
Cat. No. 403081, MicroAmp 96-Well Tray/Retainer Set – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the 7000 System, 2720 Thermal Cycler, and GeneAmp PCR System 9700.
Cat. No. 4379983, MicroAmp 96-Well Tray for VeriFlex Blocks – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes with Caps, 0.1 mL), with the Veriti Thermal Cycler, Veriti Fast Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.
Please take a look at this video: How to Use Adapter Tray Retainers in Thermal Cycler Starter Kits (https://www.youtube.com/watch?v=1uh2ZxZJtgs).
Find additional tips, troubleshooting help, and resources within our PCR Thermal Cyclers Support Center.
A guide to replacing lost calibration files for the GeneAmp PCR System 9700 can be found here: http://tools.thermofisher.com/content/sfs/brochures/cms_042718.pdf.
Please refer to the guidelines in this protocol(https://tools.thermofisher.com/content/sfs/manuals/cms_070449.pdf) specific to the GeneAmp PCR System 9700 and block type.
To add an infinite hold, set the time for the hold to ‘99.59'. The screen should then default to the infinity symbol for your hold.
No, the GeneAmp PCR System 9700 does not have a gradient feature. Our newer thermal cyclers including the ProFlex PCR System and Veriti Thermal Cycler have better-than-gradient VeriFlex Blocks.
The AutoDelta feature allows you to incrementally increase or decrease the temperature or hold time with each cycle for a given step within a cycling stage. One common application for this is touchdown PCR.
Please see the max block ramp rates in degrees/C below:
- 96-well aluminum: 2.3
- 96-well gold: 2.6
- 96-well silver: 2.6
- dual 96-well: 1.6
- dual 384-well: 1.8
- 60-well: 1.9
The plates and plastics will be specific to the block type. Please refer to the tables here (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics.html) for a list of compatible plastics, based on your block type.
The GeneAmp PCR System 9700 offers interchangeable sample blocks in a variety of well formats and materials to accommodate a wide range of applications.
96-Well Aluminum
96-Well Gold
96-Well Silver
Dual 96-Well
Dual 384-Well
60-Well