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GeneAmp® PCR System 9700 Dual 384 well - FAQs

查看更多产品信息 GeneAmp® PCR System 9700 Dual 384 well - FAQs (N8050002)

16 个常见问题解答

我应如何替换GeneAmp 9700型PCR系统中丢失的校准文件？

点击 这里(https://tools.thermofisher.com/content/sfs/brochures/cms_042718.pdf)，可查看GeneAmp 9700型PCR系统的丢失校准文件替换指南。

如何在GeneAmp 9700 PCR系统上进行温度校验？

请参考GeneAmp 9700 PCR系统及其模块类型专用的 实验方案指南(https://tools.thermofisher.com/content/sfs/manuals/cms_070449.pdf) 。

GeneAmp 9700 PCR系统能否永久维持一个恒定温度？

如需设置永久恒定温度，请将时间设置为“99.59”。屏幕将在此温度处默认显示（∞）图标。

GeneAmp 9700 PCR系统有无梯度控温的功能？

没有，GeneAmp 9700 PCR系统未整合梯度控温功能。我们的新款热循环仪 - ProFlex PCR 系统 与 Veriti 热循环仪 - 拥有超越梯度控温技术的VeriFlex模块。

GeneAmp 9700 PCR系统的Auto Delta（AutoX）特性有何作用？

Auto Delta特性能够帮助您在循环阶段中逐渐增高或降低每一循环某一步骤的温度。其中一个常见应用就是降落PCR（touchdown PCR）。

GeneAmp 9700 PCR系统的变温速率如何？

请参见下面不同模块类型的最大模块变温速率（°C/秒）：

-96孔铝制模块： 2.3
-96孔金制模块： 2.6
-96孔银制模块： 2.6
-双96孔： 1.6
-双384孔： 1.8
-60孔： 1.9

GeneAmp 9700 PCR系统适用哪些塑料孔板或反应管？

孔板与塑料耗材均为特定模块类型所专用。请参考此表(https://www.thermofisher.com/cn/zh/home/life-science/pcr/pcr-plastics.html)，了解您的模块类型兼容的塑料耗材。

GeneAmp 9700 PCR系统适用哪些类型的模块？

GeneAmp 9700 PCR系统提供多种反应孔形式和材质的可互换样本模块，适用于各类应用。

96孔铝制模块
96孔金制模块
96孔银制模块
双96孔
双384孔
60孔

How do I replace lost calibration files on the GeneAmp PCR System 9700?

A guide to replacing lost calibration files for the GeneAmp PCR System 9700 can be found here: http://tools.thermofisher.com/content/sfs/brochures/cms_042718.pdf.

How do I perform the temperature verification on the GeneAmp PCR System 9700?

Please refer to the guidelines in this protocol(https://tools.thermofisher.com/content/sfs/manuals/cms_070449.pdf) specific to the GeneAmp PCR System 9700 and block type.

How do I program an infinite hold in the GeneAmp PCR System 9700?

To add an infinite hold, set the time for the hold to ‘99.59'. The screen should then default to the infinity symbol for your hold.

Is the GeneAmp PCR System 9700 capable of running a gradient?

No, the GeneAmp PCR System 9700 does not have a gradient feature. Our newer thermal cyclers including the ProFlex PCR System and Veriti Thermal Cycler have better-than-gradient VeriFlex Blocks.

What is the Auto Delta (AutoX) feature on the GeneAmp PCR System 9700?

The AutoDelta feature allows you to incrementally increase or decrease the temperature or hold time with each cycle for a given step within a cycling stage. One common application for this is touchdown PCR.

What is the max block ramp rate of the GeneAmp PCR System 9700?

Please see the max block ramp rates in degrees/C below:
- 96-well aluminum: 2.3
- 96-well gold: 2.6
- 96-well silver: 2.6
- dual 96-well: 1.6
- dual 384-well: 1.8
- 60-well: 1.9

What types of plates or tubes can I use with the GeneAmp PCR System 9700?

The plates and plastics will be specific to the block type. Please refer to the tables here (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics.html) for a list of compatible plastics, based on your block type.

What types of blocks are available on the GeneAmp PCR System 9700?

The GeneAmp PCR System 9700 offers interchangeable sample blocks in a variety of well formats and materials to accommodate a wide range of applications.

96-Well Aluminum
96-Well Gold
96-Well Silver
Dual 96-Well
Dual 384-Well
60-Well