AmpliTaq® DNA Polymerase, Stoffel Fragment - FAQs

Do PCR products produced with Stoffel fragment have sufficient A-overhangs to work with TA Cloning kit?

Yes. Stoffel fragment is a 61 kDa modified form of AmpliTaq DNA Polymerase that lacks 5' to 3' exonuclease activity. It is more thermostable than AmpliTaq DNA Polymerase, giving it improved performance at the higher denaturating temperatures used with templates that have secondary structure. It more active over a wider range of magnesium concentrations (2 mM to 10 mM MgCl2) than Taq polymerase.