Search
Search
查看更多产品信息 DryEase™ Mini-Gel Drying System - FAQs (NI2387)
10 个常见问题解答
请参考以下可能原因和解决方案：
- 玻璃纸之间有空气： 使用充足的凝胶干燥溶液，防止凝胶和玻璃纸间产生气泡。
- 玻璃纸的张力过大： 在组装干燥系统时，注意不要拉扯玻璃纸。
- 凝胶边缘有毛边或小裂纹：修剪凝胶的毛边，从而消除裂缝的起始点。
- 干燥过程中，玻璃纸滑动： 应确保夹子牢牢固定住干燥系统的四个边缘
通常情况下，不需要剥离玻璃纸。如果您的信号非常微弱并且您担心信号获取，那么，可先使用湿毛巾摩擦凝胶的上表面，再剥离玻璃纸。然后，将凝胶通风干燥几分钟，再进行放射自显影检测。
干燥速度太快会使凝胶变白，这通常发生在过度通风或温暖的环境下。您可用Kimwipe纸在凝胶上轻轻涂去离子水，使变白部分再水化。然后在黑暗、干燥、不通风的地方再次干燥凝胶，例如抽屉或橱柜。
我们可提供以下蛋白质凝胶干燥系统：
•DryEase 小型凝胶干燥系统（货号NI2387）：简单易用的试剂盒利用被动蒸发，实现均匀、无裂纹的小型凝胶干燥，获得均匀的干燥凝胶。用该系统干燥的凝胶适用于荧光显影、密度测定、放射自显影或永久保存凝胶。
•每个DryEase 小型凝胶干燥系统含有以下组件：
◦2个DryEase 小型凝胶干燥框（包括2个框架和8个夹子）（货号NI2380）
◦1个DryEase 小型凝胶干燥基座（货号NI2300）
◦200张DryEase 小型预切玻璃纸（货号NC2380）
◦500 mL Gel-Dry 干燥液（货号NI2100）
•大型凝胶干燥试剂盒（货号NI2207）：可提供与DryEase 小型凝胶干燥系统相同的均匀、无裂纹凝胶干燥，可容纳大型凝胶或4块小型凝胶。该系统无需基座。
每个大型凝胶干燥试剂盒都含有以下组件：
◦1个-DryEase 大型凝胶干燥框（包括8个夹子）（货号NI2200）
◦100张DryEase 大型预切玻璃纸（货号NC2200）
◦500 mL Gel-Dry 干燥液（货号LC4025）
我们可提供Invitrogen凝胶上样滴管，使上样简单、精确。它们是无RNase/DNase和非高温高压消毒的。我们可提供3个品种：标准圆形滴管、平头凝胶滴管和Eppendorf圆形凝胶滴管。
Please see below for possible causes and solutions:
- Air trapped between cellophane layers. Apply adequate Gel-Drying solution to prevent bubbles between the gel and cellophane assembly.
- Tension on cellophane too strong. Take care not to stretch the cellophane during dryer assembly.
- Rough edges or small cracks on gel edges. Trim rough edges from the gel to eliminate starting points for cracks.
- Cellophane slips during drying. Make sure clamps hold all four edges of the dryer assembly firmly.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Usually it is not necessary to peel off the cellophane. If your signal is very faint and you are concerned about getting an exposure, you can rub the top surface of your gel with a damp towel and then peel off the cellophane. Then air dry the gel for a few minutes and perform autoradiography.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Gels turn white when they are dried too rapidly, usually in an environment that is too ventilated, drafty, or warm. You can remedy this problem by dabbing the gel with deionized water on a Kimwipe tissue to rehydrate the white areas. Re-dry the gel in a dark, dry, non-drafty place, such as a drawer or cabinet.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The pore size of DryEase cellophane is 40 microns.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
We offer the following systems for drying of protein gels:
DryEase Mini Gel Drying System (Cat. No. NI2387): Simple-to-use kit for even, crack-free drying of mini gels that dries gels evenly, using passive evaporation. Gels dried with this system are suitable for fluorography, densitometry, autoradiography, or permanent gel storage. Each DryEase Mini-Gel Drying System contains the following components:
- 2-DryEase Mini-Gel Drying Frames (includes 2 frames + 8 clamps) (Cat. No. NI2380)
- 1-DryEase Mini-Gel Drying Base (Cat. No. NI2300)
- 200-DryEase Mini Cellophane precut sheets (Cat. No. NC2380)
- 500 mL Gel-Dry Drying Solution (Cat. No. NI2100)
Large Gel Drying Kit (Cat. No. NI2207): Provides the same even, crack-free gel drying seen with the DryEase Mini-Gel Drying System but is designed to accommodate larger gels or 4 mini-gels. A base for the system is not required.
Each Large Gel Drying Kit contains the following components:
- 1-DryEase Large Gel Drying Frame (includes 8 clamps) (Cat. No. NI2200)
- 100-DryEase Large Cellophane precut sheets (Cat. No. NC2200)
- 500 mL Gel-Dry Drying Solution (Cat. No. LC4025)
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.