NuPAGE™ MES SDS 电泳缓冲液 (20X)
Invitrogen17万+抗体限时买二赠一，靶点广，灵活用！
Femto超敏底物钜惠大包装上市！100mL到手价不到500元!
NuPAGE™ MES SDS 电泳缓冲液 (20X)
引用和文献 (5)
Invitrogen™

NuPAGE™ MES SDS 电泳缓冲液 (20X)

NuPAGE MES SDS 电泳缓冲液 (20X) 适用于使用 NuPAGE Bis-Tris 凝胶的蛋白电泳。推荐用于分离低至中分子量的蛋白质了解更多信息
Have Questions?
更改视图buttonViewtableView
货号数量
NP0002500mL
NP0002025L
货号 NP0002
价格（CNY)
580.00
飞享价
Ends: 31-Dec-2025
918.00
共减 338.00 (37%)
Each
添加至购物车
数量:
500mL
价格（CNY)
580.00
飞享价
Ends: 31-Dec-2025
918.00
共减 338.00 (37%)
Each
添加至购物车
NuPAGE MES SDS 电泳缓冲液 (20X) 适用于使用 NuPAGE Bis-Tris 凝胶的蛋白电泳。推荐用于分离低至中分子量的蛋白质。

选用合适的缓冲液，实现理想的蛋白分离
NuPAGE MES SDS 电泳缓冲液和 NuPAGE MOPS SDS 电泳缓冲液都可与 NuPAGE Bis-Tris 凝胶搭配使用。MES 具有更低的 pKa，使得 MES SDS 电泳缓冲液的速度比 MOPS SDS 电泳缓冲液更快。不同缓冲液的离子迁移效应的差异会影响蛋白堆叠，进而导致蛋白分离范围的差异。使用 MOPS 缓冲液时，蛋白迁移速度较使用 MES 缓冲液时更慢。

使用 MES 和 MOP 缓冲液时 NuPAGE Bis-Tris 凝胶上的蛋白迁移模式对比

查看所有 SDS-PAGE 缓冲液和试剂

仅供科研使用。不可用于诊断程序。
规格
化学名称或材料电泳缓冲液
配方1X：50 mM MES、50 mM Tris Base、0.1% SDS、1 mM EDTA，pH 7.3。
建议的储存条件内含：NuPAGE™ MES SDS 电泳缓冲液 (20X)
储存条件：+4°C 至 25°C
可溶性信息Water
最大浓度20 X
物理形态Liquid
产品线NuPAGE
数量500mL
pHpH 7.3
Unit SizeEach

常见问题解答 (FAQ)

我能否一起使用NuPAGE Bis-Tris凝胶和无SDS的NuPAGE MOPS或MES上样缓冲液，在非变性条件下电泳？

我们不推荐一起使用NuPAGE Bis-Tris凝胶和无SDS的NuPAGE MOPS或MES上样缓冲液在非变性条件下电泳。这种缓冲液系统会产生过多热量，导致条带分辨率较差。此外，在中性pH环境下，不带电荷的目标蛋白可能不会很好地迁移。

NuPAGE MOPS和MES SDS电泳缓冲液能否兼容通过Edman降解进行的蛋白质N端测序？

NuPAGE MOPS和MES SDS电泳缓冲液可兼容Edman蛋白质测序。可直接对NuPAGE凝胶上的蛋白质测序。

NuPAGE缓冲液应如何保存？

NuPAGE缓冲液应保存在4–25℃。

使用你们的预制蛋白质凝胶时，推荐的样品上样体积和蛋白质上样量是多少？

•Invitrogen Tris-甘氨酸和InvitrogenTricine小型凝胶：Invitrogen预制胶电泳指南（https://tools.thermofisher.com/content/sfs/manuals/electrophoresisguide_man.pdf），第8页

•NuPAGE Tris-Acetate和NuPAGE Bis-Tris小型凝胶：NuPAGE技术指南（https://tools.thermofisher.com/content/sfs/manuals/nupage_tech_man.pdf），第10页

•Bolt Bis-Tris Plus小型凝胶：点击此处（https://www.thermofisher.com/us/en/home/life-science/protein-expression-and-analysis/protein-gel-electrophoresis/protein-gels/bolt-bis-tris-gels.html）查看

•Thermo Scientific Precise Tris-HEPES凝胶：Precise Tris-HEPES凝胶使用手册（https://tools.thermofisher.com/content/sfs/manuals/MAN0011499_Precise_Protein_Gels_UG.pdf），第1页

•中型凝胶（Invitrogen Tris-甘氨酸、NuPAGE Bis-Tris和NuPAGE Tris-Acetate）：Invitrogen中型凝胶系统使用手册（https://tools.thermofisher.com/content/sfs/manuals/Invitrogen_midigel_man.pdf），第4页

•Thermo Scientific Precise Tris-甘氨酸凝胶：Precise Tris-甘氨酸凝胶使用手册（https://tools.thermofisher.com/content/sfs/manuals/MAN0011814_Precise_TrisGlycine_Gels_UG.pdf），第1页

你们的预制蛋白质凝胶是否含有SDS？

我们的预制蛋白质凝胶不含SDS，但在使用合适的变性电泳缓冲液时，可在变性条件下电泳。
注意：NuPAGE Bis-Tris凝胶、Bolt Bis-Tris Plus凝胶和Thermo Scientific Precise Tris-HEPES凝胶不可在非变性条件下电泳；这些凝胶只能用于变性条件下。

*Invitrogen Tris-甘氨酸凝胶：非变性电泳时使用Invitrogen Tris-甘氨酸非变性电泳缓冲液 。变性电泳时使用 Invitrogen Tris-甘氨酸SDS电泳缓冲液

*Invitrogen NuPAGE Tris-Acetate凝胶：非变性电泳时使用Invitrogen Tris-甘氨酸非变性电泳缓冲液 。变性电泳时使用 NuPAGE Tris-乙酸SDS电泳缓冲液

*Invitrogen NuPAGE Bis-Tris凝胶：使用NuPAGE MOPS-SDS电泳缓冲液或NuPAGE MES-SDS电泳缓冲液进行变性电泳

*Invitrogen Bolt Bis-Tris Plus凝胶：使用Bolt MOPS SDS电泳缓冲液或Bolt MES SDS电泳缓冲液进行变性电泳

*Thermo Scientific Precise Tris-甘氨酸凝胶：非变性电泳时使用Tris-甘氨酸SDS电泳缓冲液，不加入SDS。变性电泳时使用Tris-甘氨酸SDS电泳缓冲液。

*Thermo Scientific Precise Tris-HEPES凝胶：使用Tris-HEPES SDS电泳缓冲液进行变性电泳。

View all NuPAGE™ MES SDS 电泳缓冲液 (20X) FAQs

引用和文献 (5)

引用和文献
Abstract
Matrix GLA protein, a regulatory protein for bone morphogenetic protein-2.
Authors: Zebboudj Amina F; Imura Minori; Boström Kristina;
Journal:J Biol Chem
PubMed ID:11741887
'Matrix GLA protein (MGP) has been identified as a calcification inhibitor in cartilage and vasculature. Part of this effect may be attributed to its influence on osteoinductive activity of bone morphogenetic protein-2 (BMP-2). To detect binding between MGP and BMP-2, we performed immunoprecipitation using MGP and BMP-2 tagged with FLAG ... More
Oligomerization of the integrin alphaIIbbeta3: roles of the transmembrane and cytoplasmic domains.
Authors: Li R; Babu C R; Lear J D; Wand A J; Bennett J S; DeGrado W F;
Journal:Proc Natl Acad Sci U S A
PubMed ID:11606749
'Integrins are a family of alpha/beta heterodimeric membrane proteins, which mediate cell-cell and cell-matrix interactions. The molecular mechanisms by which integrins are activated and cluster are currently poorly understood. One hypothesis posits that the cytoplasmic tails of the alpha and beta subunits interact strongly with one another in a 1:1 ... More
beta -Glucoside Kinase (BglK) from Klebsiella pneumoniae. PURIFICATION, PROPERTIES, AND PREPARATIVE SYNTHESIS OF 6-PHOSPHO-beta -D-GLUCOSIDES.
Authors: Thompson John; Lichtenthaler Frieder W; Peters Siegfried; Pikis Andreas;
Journal:J Biol Chem
PubMed ID:12110692
ATP-dependent beta-glucoside kinase (BglK) has been purified from cellobiose-grown cells of Klebsiella pneumoniae. In solution, the enzyme (EC ) exists as a homotetramer composed of non-covalently linked subunits of M(r) approximately 33,000. Determination of the first 28 residues from the N terminus of the protein allowed the identification and cloning ... More
An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system.
Authors: Kiga Daisuke; Sakamoto Kensaku; Kodama Koichiro; Kigawa Takanori; Matsuda Takayoshi; Yabuki Takashi; Shirouzu Mikako; Harada Yoko; Nakayama Hiroshi; Takio Koji; Hasegawa Yoshinori; Endo Yaeta; Hirao Ichiro; Yokoyama Shigeyuki;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12097643
Tyrosyl-tRNA synthetase (TyrRS) from Escherichia coli was engineered to preferentially recognize 3-iodo-L-tyrosine rather than L-tyrosine for the site-specific incorporation of 3-iodo-L-tyrosine into proteins in eukaryotic translation systems. The wild-type TyrRS does not recognize 3-iodo-L-tyrosine, because of the bulky iodine substitution. On the basis of the reported crystal structure of Bacillus ... More
Adverse drug reactions.
Authors:Patton K,Borshoff DC
Journal:Anaesthesia
PubMed ID:29313907