Interaction of HIV-1 Integrase with DNA Repair Protein hRad18.
Authors Mulder Lubbertus C F; Chakrabarti Lisa A; Muesing Mark A;
JournalJ Biol Chem
PubMed ID12016221
'We have previously shown that human immunodeficiency virus-1 (HIV-1) integrase is an unstable protein and a substrate for the N-end rule degradation pathway. This degradation pathway shares its ubiquitin-conjugating enzyme, Rad6, with the post-replication/translesion DNA repair pathway. Because DNA repair is thought to play an essential role in HIV-1 integration, ... More
Oligomerization of the fifth transmembrane domain from the adenosine A2A receptor.
AuthorsThévenin D, Lazarova T, Roberts MF, Robinson CR,
JournalProtein Sci
PubMed ID15987888
The human adenosine A2A receptor (A(2A)R) belongs to one of the largest family of membrane proteins, the G-protein coupled receptors (GPCRs), characterized by seven transmembrane (TM) helices. Little is known about the determinants of their structures, folding, assembly, activation mechanisms, and oligomeric states. Previous studies in our group showed that ... More
Covalent modification regulates ligand binding to receptor complexes in the chemosensory system of Escherichia coli.
AuthorsLi G, Weis RM
JournalCell
PubMed ID10676817
In the Escherichia coli chemosensory pathway, receptor modification mediates adaptation to ligand. Evidence is presented that covalent modification influences ligand binding to receptors in complexes with CheW and the kinase CheA. Kinase inhibition was measured with serine receptor complexes in different modification levels; Ki for serine-mediated inhibition increased 10,000-fold from ... More
Changes in the glycosylation pattern of prion protein in murine scrapie. Implications for the mechanism of neurodegeneration in prion diseases.
AuthorsRusselakis-Carneiro M, Saborio GP, Anderes L, Soto C,
JournalJ Biol Chem
PubMed ID12130636
In prion diseases, the normal prion protein (PrP(c)) undergoes a conformational change that results in the abnormal form, named scrapie prion protein (PrP(sc)). The visual system of rodents provides a relatively simple neuronal model in which the cell bodies of neurons are confined to the retina and the axons constitute ... More
Purification and characterization of a membrane-bound hydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus
AuthorsSapra R, Verhagen MF, Adams MW
JournalJ Bacteriol
PubMed ID10852873
Highly washed membrane preparations from cells of the hyperthermophilic archaeon Pyrococcus furiosus contain high hydrogenase activity (9.4 micromol of H(2) evolved/mg at 80 degrees C) using reduced methyl viologen as the electron donor. The enzyme was solubilized with n-dodecyl-beta-D-maltoside and purified by multistep chromatography in the presence of Triton X-100. ... More
Characterization of the Structure, Function, and Conformational Stability of PorB Class 3 Protein from Neisseria meningitidis
AuthorsConceicao, A. S. A Minetti, M. S. Blake and David P. Remeta
JournalJ Biol Chem
PubMed ID9737999
PorB proteins constitute the vast majority of channels in neisserial outer membranes and can be subdivided within meningococcal strains into two distinct and mutually exclusive families that are designated as class 2 and class 3 proteins. We recently characterized the functional activity and conformational stability of a PorB class 2 ... More
Angiogenic bFGF expression from gas-plasma treated scaffolds.
AuthorsBailey SR, Polan JL, Morse B, Wetherold S, Villanueva-Vedia RE, Waggoner D, Phelix C, Barera-Roderiquiz E, Goswami N, Munoz O, Agrawal CM,
JournalCardiovasc Radiat Med
PubMed ID12974371
PURPOSE: In vivo experiments indicate that gas-plasma-treated D,L-polylactide polymers expressing basic fibroblast growth factor (bFGF) exhibit enhanced angiogenesis. bFGF is not a single entity, but it is instead a family of isoforms. Consequently, we sought to determine which bFGF isoforms and levels initiate angiogenesis in nude mice peritoneums. METHODS: Cytoplasmic ... More
Effect of bronchoalveolar lavage fluid from Pneumocystis carinii-infected hosts on phagocytic activity of alveolar macrophages.
AuthorsLasbury ME, Lin P, Tschang D, Durant PJ, Lee CH,
JournalInfect Immun
PubMed ID15039336
Alveolar macrophages from Pneumocystis carinii-infected rats are defective in phagocytosis. To investigate whether this defect is due to a certain factor present in P. carinii-infected lungs, alveolar macrophages from uninfected rats were incubated with bronchoalveolar lavage (BAL) fluid samples from P. carinii-infected rats. Alveolar macrophages treated with these BAL fluid ... More
A regulatory light chain of ciliary outer arm dynein in Tetrahymena thermophila.
AuthorsChristensen ST, Guerra C, Wada Y, Valentin T, Angeletti RH, Satir P, Hamasaki T,
JournalJ Biol Chem
PubMed ID11274140
Ciliary beat frequency is primarily regulated by outer arm dyneins (22 S dynein). Chilcote and Johnson (Chilcote, T. J., and Johnson, K. A. (1990) J. Biol. Chem. 256, 17257-17266) previously studied isolated Tetrahymena 22 S dynein, identifying a protein p34, which showed cAMP-dependent phosphorylation. Here, we characterize the molecular biochemistry ... More
Identification and expression of immunogenic proteins of a disease-associated marine turtle herpesvirus.
AuthorsCoberley SS, Condit RC, Herbst LH, Klein PA,
JournalJ Virol
PubMed ID12239336
Herpesviruses are associated with several diseases of marine turtles, including lung-eye-trachea disease (LETD) and fibropapillomatosis. Two approaches were used to identify immunodominant antigens of LETV, the LETD-associated herpesvirus. The first approach targeted glycoprotein B, which is known to be immunogenic and neutralizing in other species. The second strategy identified LETV ... More
Rapid exchange of actin-bound nucleotide in perfused rat heart.
AuthorsBárány M, de Tombe PP,
JournalAm J Physiol Heart Circ Physiol
PubMed ID15020303
In the rat heart the actin-bound nucleotide contained both ATP and ADP. The ratio of bound ATP to bound ADP depended on the functional state of the heart; it was higher in hearts stopped reversibly in diastole (low Ca(2+), high Mg(2+), or high K(+)), than in stimulated (inotropic agents or ... More
Efficient intracellular assembly of papillomaviral vectors.
Although the papillomavirus structural proteins, L1 and L2, can spontaneously coassemble to form virus-like particles, currently available methods for production of L1/L2 particles capable of transducing reporter plasmids into mammalian cells are technically demanding and relatively low-yield. In this report, we describe a simple 293 cell transfection method for efficient ... More
The myostatin propeptide and the follistatin-related gene are inhibitory binding proteins of myostatin in normal serum.
Authors Hill Jennifer J; Davies Monique V; Pearson Adele A; Wang Jack H; Hewick Rodney M; Wolfman Neil M; Qiu Yongchang;
JournalJ Biol Chem
PubMed ID12194980
Myostatin, also known as growth and differentiation factor 8, is a member of the transforming growth factor beta superfamily that negatively regulates skeletal muscle mass (1). Recent experiments have shown that myostatin activity is detected in serum by a reporter gene assay only after activation by acid, suggesting that native ... More
Neutrophils employ the myeloperoxidase system to generate antimicrobial brominating and chlorinating oxidants during sepsis.
Authors Gaut J P; Yeh G C; Tran H D; Byun J; Henderson J P; Richter G M; Brennan M L; Lusis A J; Belaaouaj A; Hotchkiss R S; Heinecke J W;
JournalProc Natl Acad Sci U S A
PubMed ID11593004
The myeloperoxidase system of neutrophils uses hydrogen peroxide and chloride to generate hypochlorous acid, a potent bactericidal oxidant in vitro. In a mouse model of polymicrobial sepsis, we observed that mice deficient in myeloperoxidase were more likely than wild-type mice to die from infection. Mass spectrometric analysis of peritoneal inflammatory ... More