Bolt™ Bis-Tris Plus Welcome Pack and Mini Blot Modules, 4–12%

The Bolt Bis-Tris Plus Welcome Pack contains:
• Mini Gel Tank (Cat. No. A25977)
• Bolt Bis-Tris Plus gels (2 boxes, 20 gels)
• Bolt MES Running Buffer, 20X (Cat. No. B0002)
• Bolt LDS Sample Buffer, 4X (Cat. No. B0007)
• Bolt Sample Reducing Agent, 10X (Cat. No. B0009)
• PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa (Cat. No. 26619)
• 2 x Mini Blot Modules (Cat. No. B1000)

About the Mini Gel Tank
The Mini Gel Tank is compatible with Novex gels, NuPAGE gels, and Bolt Bis-Tris Plus gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt Bis-Tris Plus gels to be run in approximately 20 minutes. Run times may vary depending on buffer conditions and power supplies used.

About Bolt Bis-Tris Plus Gels
Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8, 10, 12, and 4-12% gradient and multiple well formats. Bolt Bis-Tris gels feature wedge-shaped wells, which can easily load up to two times more sample volume allowing for detection of proteins in very dilute samples or visualization of low-abundance proteins.

Transfer
For transferring your proteins to a membrane, we recommend using for Bolt Transfer Buffer (Cat. No. BT0006) for traditional wet transfer using the Mini Blot Module (Cat. No. B1000).

About the Mini Blot Module
The Mini Blot Module is a wet transfer device used exclusively with the Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. The Mini Blot Module is compatible all Invitrogen mini gels (blot size: 8.5 x 8 cm). The blot module accommodates one mini gel per wet transfer. The universal connection and molded gasket make the module easy to use, while the inner core of the module allows for use of less methanol-based transfer buffer per wet transfer (250 mL per gel). At the recommended conditions and constant voltage, proteins can be transferred to nitrocellulose or PVDF membranes typically in 30 to 60 minutes.