Mini Gel Tank and Blot Module Set - FAQs

我们建议在将凝胶盒放置在电泳槽中之前，使用记号笔在凝胶盒上标记出孔的底部。

以下是可能原因和解决方案：

- 凝胶盒底部留有胶带。应从凝胶盒底部去除胶带。
- 与电源连接不良。使用电压表检测所有连接处的电导率。
- 缓冲液不足。应确保电泳槽中的缓冲液足以浸没凝胶的上样孔。

以下是可能原因和解决方案：

- 使用了浓度过高或错误的缓冲液。应检查缓冲液配方；必要时，稀释或重新配制。
- 电流设置过高。将电流降低至推荐的电泳条件（见使用手册第8页）。

以下是可能原因和解决方案：

- 缓冲液稀释过度。检查缓冲液配方；必要时，重新配制。
- 缓冲液槽泄漏。确保凝胶盒夹安置稳固，垫片在原位，并且凝胶盒夹已锁定。
- 电流设置过低。设置正确的电流。

以下是我们可提供的小型凝胶电泳槽替换部件：

替换部件 - 货号

凝胶电泳槽 - B4478641
小型凝胶电泳槽盖 - A25944
小型凝胶电泳槽基座 - A25950
凝胶盒夹，左 - A25946
凝胶盒夹，右 - A25945
凝胶盒夹凸轮手柄装置 - A26732

我们建议：

•电泳完毕后，妥善处理电泳缓冲液。用水冲洗电泳槽以除去残留的缓冲液。
•用蘸过水的柔软非摩擦性无尘布清洁小型凝胶电泳槽的表面。
•不要使用强力洗涤剂或溶剂清洗装置。

小型凝胶电泳槽不兼容氯化烃（如氯仿）、芳香烃（如甲苯和苯）或丙酮。

在使用转接板的情况下，Precise Tris-HEPES凝胶可兼容XCell SureLock Mini Cell或小型凝胶电泳槽。

注意：使用XCell SureLock Mini Cell或小型凝胶电泳槽时，1块凝胶需要2个转接板，2块凝胶需要1个转接板。

我们的传统Bolt Bis-Tris Plus小型凝胶（货号BGxxxxxBOX，2014年12月31日起停产）只能使用Bolt小型凝胶电泳槽（2014年12月31日起停产，库存售尽后将停止供应）进行电泳。

我们的新型Bolt Bis-Tris Plus小型凝胶（货号NWxxxxxBOX）以及Invitrogen小型凝胶和NuPAGE小型凝胶，可使用小型凝胶电泳槽或XCell SureLock Mini-Cell。若这些凝胶使用Bolt小型凝胶电泳槽（2014年12月31日起停产）进行电泳，则有必要升级电泳槽，可将黑色10.5 cm凝胶盒夹凸轮手柄更换为灰色10 cm凝胶盒夹凸轮手柄（货号A26732，凝胶盒夹凸轮手柄装置）。点击此处（https://www.thermofisher.com/us/en/home/life-science/protein-expression-and-analysis/protein-gel-electrophoresis/electrophoresis-chambers/mini-gel-tank/bolt-mini-gel-tank-resources.html）或视频（https://www.youtube.com/watch?v=1FtiX8Skllw），可查看凸轮手柄更换说明。

我们的中型凝胶可使用XCell4 SureLock Midi-Cell进行电泳。

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

No, most transfer applications require high current and therefore, we recommend using a high current power supply like PowerEase Touch 350W Power Supply.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

No. Bio-Rad gels are of a different size (length, width, and depth) and have a special feature that precludes them from fitting in the Mini Gel Tank.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

All Novex, NuPAGE, and Bolt mini gels can be run in the Mini Gel Tank, including:
- NuPAGE Tris-Acetate Protein Gels
- 10 cm Bolt Bis-Tris Plus Gels
- NuPAGE Bis-Tris Protein Gels
- Novex Tricine Protein Gels

Note: Refer to the user manual for specific run conditions for each gel type.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The Mini Gel Tank includes the Runner Tank, Cassette Clamps (left & right), Tank Base, Tank Lid, and Power Supply Adapters.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

We recommend marking the cassette at the bottom of the wells with a marker pen prior to placing the cassette in the electrophoresis tank.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Here are possible causes and solutions:

- Tape left on the bottom of the cassette. Remove tape from bottom of cassette.
- Connection to power supply not complete. Check all connections with a voltmeter for conductance.
- Insufficient buffer level. Make sure there is sufficient buffer in the electrophoresis tank to cover the wells of the gel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Here are possible causes and solutions:

- Buffers are too concentrated or incorrect. Check buffer recipe; dilute or re-make if necessary.
- Current is set at a higher limit. Decrease current to recommended running conditions (see Page 8 of the manual).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Here are possible causes and solutions:

- Buffers are too dilute. Check buffer recipe; remake if necessary.
- Buffer chamber is leaking. Make sure the cassette clamp is firmly seated, the gaskets are in place and the cassette clamp is locked.
- Current is set too low. Set correct current.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Here are the replacement parts we offer for the Mini Gel Tank:
Replacement part - Cat. No.
Gel Runner Tank - B4478641
Mini Gel Tank Lid - A25944
Mini Gel Tank Base - A25950
Cassette Clamp, left - A25946
Cassette Clamp, right - A25945
Cassette Clamp Cam Handle Set - A26732

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Here are our recommendations:

- When electrophoresis is complete, dispose of the buffer appropriately. Rinse the tank with water to remove residual buffer.
- Clean the surface of the Mini Gel Tank with a soft non-abrasive, lint-free cloth dampened with water.
- Do not use harsh detergents or solvents to clean the unit.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The Mini Gel Tank is not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene) or acetone.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The Precise Tris-HEPES gels are compatible with the XCell SureLock Mini Cell or Mini Gel Tank when used with adaptor plates. Note: Two adaptor plates are required when running just one gel and one adaptor plate is required when running two gels using the XCell SureLock Mini Cell or Mini Gel Tank.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Our Original Bolt Bis-Tris Plus Mini gels (Cat. No. BGxxxxxBOX, discontinued as of December 31, 2014) can only be run in the Bolt Mini Gel Tank (discontinued as of December 31, 2014, and will be offered until inventory is depleted).

Our New Bolt Bis-Tris Plus Mini gels (Cat. No. NWxxxxxBOX), as well as our Invitrogen Mini gels and NuPAGE Mini gels can be run using the Mini Gel Tank, or XCell SureLock Mini-Cell. To run these gels using the Bolt Mini Gel Tank (discontinued as of December 31, 2014), upgrading of the tank is necessary by replacing the black 10.5 cm cassette clamp cam handles with gray 10 cm cassette clamp cam handles (Cat. No. A26732, Cassette Clamp Cam Handle Set). Instructions for replacement of the cam handles can be found here (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gel-electrophoresis-chamber-systems/mini-gel-tank/resources-upgrading-bolt-mini-gel-tank.html).

Our Midi gels can be run using the XCell4 SureLock Midi-Cell.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.