Bright monomeric red fluorescent protein with an extended fluorescence lifetime.
AuthorsMerzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, Fradkov AF, Gaintzeva A, Lukyanov KA, Lukyanov S, Gadella TW, Chudakov DM,
JournalNat Methods
PubMed ID17572680
'Fluorescent proteins have become extremely popular tools for in vivo imaging and especially for the study of localization, motility and interaction of proteins in living cells. Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high pH-stability. ... More
C-peptide is internalised in human endothelial and vascular smooth muscle cells via early endosomes.
AuthorsLuppi P, Geng X, Cifarelli V, Drain P, Trucco M,
JournalDiabetologia
PubMed ID19662378
AIMS/HYPOTHESIS: There is increasing evidence that C-peptide exerts intracellular effects in a variety of cells and could be beneficial in patients with type 1 diabetes. Exactly how C-peptide achieves these effects, however, is unknown. Recent reports showed that C-peptide internalised in the cytoplasm of HEK-293 and Swiss 3T3 cells, where ... More
Absolute two-photon absorption spectra and two-photon brightness of orange and red fluorescent proteins.
AuthorsDrobizhev M, Tillo S, Makarov NS, Hughes TE, Rebane A,
JournalJ Phys Chem B
PubMed ID19127988
Fluorescent proteins with long emission wavelengths are particularly attractive for deep tissue two-photon microscopy. Surprisingly, little is known about their two-photon absorption (2PA) properties. We present absolute 2PA spectra of a number of orange and red fluorescent proteins, including DsRed2, mRFP, TagRFP, and several mFruit proteins, in a wide range ... More
Insoluble, speckled cytosolic distribution of retinoic acid receptor alpha protein as a marker of hepatic stellate cell activation in vitro.
AuthorsMezaki Y, Yamaguchi N, Yoshikawa K, Miura M, Imai K, Itoh H, Senoo H,
JournalJ Histochem Cytochem
PubMed ID19332432
Hepatic stellate cells (HSCs) are the major site of retinoid storage, and their activation is a key process in liver fibrogenesis. We have previously shown that expression of the retinoic acid receptor alpha (RARalpha) is upregulated in activated rat HSCs at a posttranscriptional level and that these RARalpha proteins showed ... More