Bright monomeric red fluorescent protein with an extended fluorescence lifetime.
AuthorsMerzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, Fradkov AF, Gaintzeva A, Lukyanov KA, Lukyanov S, Gadella TW, Chudakov DM,
JournalNat Methods
PubMed ID17572680
'Fluorescent proteins have become extremely popular tools for in vivo imaging and especially for the study of localization, motility and interaction of proteins in living cells. Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high pH-stability. ... More
S-acylation of LCK protein tyrosine kinase is essential for its signalling function in T lymphocytes.
AuthorsKabouridis PS, Magee AI, Ley SC,
JournalEMBO J
PubMed ID9305640
LCK is a non-receptor protein tyrosine kinase required for signal transduction via the T-cell antigen receptor (TCR). LCK N-terminus is S-acylated on Cys3 and Cys5, in addition to its myristoylation on Gly2. Here the role of S-acylation in LCK function was examined. Transient transfection of COS-18 cells, which express a ... More
Absolute two-photon absorption spectra and two-photon brightness of orange and red fluorescent proteins.
AuthorsDrobizhev M, Tillo S, Makarov NS, Hughes TE, Rebane A,
JournalJ Phys Chem B
PubMed ID19127988
Fluorescent proteins with long emission wavelengths are particularly attractive for deep tissue two-photon microscopy. Surprisingly, little is known about their two-photon absorption (2PA) properties. We present absolute 2PA spectra of a number of orange and red fluorescent proteins, including DsRed2, mRFP, TagRFP, and several mFruit proteins, in a wide range ... More