Optical properties and application of a reactive and bioreducible thiol-containing tetramethylrhodamine dimer.
Authors:Christie RJ, Tadiello CJ, Chamberlain LM, Grainger DW,
Journal:Bioconjug Chem
PubMed ID:19249862
'Thiolated dimeric tetramethylrhodamine (TAMRA) was synthesized in a straightforward procedure utilizing commercially available 5(6)-succinimidyl TAMRA and cystamine hydrochloride. The thiol-containing TAMRA dimer displayed distinct spectral properties in reduced and oxidized forms; covalent dimer formation produced greater effects on the spectral properties than previously reported for noncovalent TAMRA dimers or dimers ... More