'Zero mode waveguides (ZMWs), subwavelength optical nanostructures with dimensions ranging from 50 to 200 nm, have been used to study systems involving ligand-receptor interactions. We show that under proper conditions, lipid membranes will invaginate into the nanostructures, which confine optical excitation to subattoliter volumes. Fluorescence correlation spectroscopy (FCS) was used ... More
Early endosomal SNAREs form a structurally conserved SNARE complex and fuse liposomes with multiple topologies.
AuthorsZwilling D, Cypionka A, Pohl WH, Fasshauer D, Walla PJ, Wahl MC, Jahn R
JournalEMBO J
PubMed ID17159904
'SNARE proteins mediate membrane fusion in eukaryotic cells. They contain conserved SNARE motifs that are usually located adjacent to a C-terminal transmembrane domain. SNARE motifs spontaneously assemble into four helix bundles, with each helix belonging to a different subfamily. Liposomes containing SNAREs spontaneously fuse with each other, but it is ... More
Surface-coupled proton exchange of a membrane-bound proton acceptor.
Proton-transfer reactions across and at the surface of biological membranes are central for maintaining the transmembrane proton electrochemical gradients involved in cellular energy conversion. In this study, fluorescence correlation spectroscopy was used to measure the local protonation and deprotonation rates of single pH-sensitive fluorophores conjugated to liposome membranes, and the ... More
Effects of linker sequences on vesicle fusion mediated by lipid-anchored DNA oligonucleotides.
AuthorsChan YH, van Lengerich B, Boxer SG,
JournalProc Natl Acad Sci U S A
PubMed ID19164559
Synthetic lipid-oligonucleotide conjugates inserted into lipid vesicles mediate fusion when one population of vesicles displays the 5'-coupled conjugate and the other the 3'-coupled conjugate, so that anti-parallel hybridization allows the membrane surfaces to come into close proximity. Improved assays show that lipid mixing proceeds more quickly and to a much ... More
Interplay between H1N1 influenza a virus infection, extracellular and intracellular respiratory tract pH, and host responses in a mouse model.
Authors
JournalPLoS One
PubMed ID33979408
Soft Polydimethylsiloxane-Supported Lipid Bilayers for Studying T Cell Interactions.
Authors
JournalBiophys J
PubMed ID33248128
The Rab11-binding protein RELCH/KIAA1468 controls intracellular cholesterol distribution.