Scanning near-field fluorescence resonance energy transfer microscopy.
AuthorsVickery SA, Dunn RC
JournalBiophys J
PubMed ID10096880
'A new microscopic technique is demonstrated that combines attributes from both near-field scanning optical microscopy (NSOM) and fluorescence resonance energy transfer (FRET). The method relies on attaching the acceptor dye of a FRET pair to the end of a near-field fiber optic probe. Light exiting the NSOM probe, which is ... More
Anoxia influences the lateral diffusion of a lipid probe in the plasma membrane of isolated cardiac myocytes.
AuthorsFinch SA, Piper HM, Spieckermann PG, Stier A
JournalBasic Res Cardiol
PubMed ID3994636
'Using the technique of fluorescence photobleaching recovery we have measured the characteristics of lateral diffusion of oleylaminofluorescein (OAF) in the plasma membrane of isolated rat cardiac myocytes under normoxic and anoxic conditions. The normoxic pattern is one of slow diffusion and low recovery (D = 1.8 +/- 0.3 X 10(-10)cm2/s, ... More
A role for second messengers in the control of activation-associated modification of the surface of Trichinella spiralis infective larvae.
AuthorsModha J, Kusel JR, Kennedy MW
JournalMol Biochem Parasitol
PubMed ID8538685
'The involvement of second messengers in the control of activation-induced changes to the surface of Trichinella spiralis infective larvae was investigated using membrane-permeant photo-activatable ''caged'' compounds to alter intracellular levels of inositol trisphosphate (IP3), calcium ions (Ca2+) and cyclic AMP (cAMP). Activation of larvae by incubation in culture medium containing ... More
Insertion of fluorescent fatty acid probes into the outer membranes of the pathogenic spirochaetes Treponema pallidum and Borrelia burgdorferi.
AuthorsCox DL, Radolf JD
JournalMicrobiology
PubMed ID11320119
'The authors examined the ability of octadecanoyl (C(18)), hexadecanoyl (C(16)) and dodecanoyl (C(12)) fatty acid (FA) conjugates of 5-aminofluorescein (OAF, HAF and DAF, respectively) to insert into the outer membranes (OMs) of Treponema pallidum, Borrelia burgdorferi and Escherichia coli. Biophysical studies have demonstrated that these compounds stably insert into phospholipid ... More
Self diffusion and spectral modifications of a membrane protein, the Rubrivivax gelatinosus LH2 complex, incorporated into a monoolein cubic phase.
AuthorsTsapis N, Reiss-Husson F, Ober R, Genest M, Hodges RS, Urbach W
JournalBiophys J
PubMed ID11509374
'The light-harvesting complex LH2 from a purple bacterium, Rubrivivax gelatinosus, has been incorporated into the Q230 cubic phase of monoolein. We measured the self-diffusion of LH2 in detergent solution and in the cubic phase by fluorescence recovery after photobleaching. We investigated also the absorption and fluorescence properties of this oligomeric ... More
Effects of phytohormones on the surfaces of plant-parasitic nematodes.
AuthorsAkhkha A, Kusel J, Kennedy M, Curtis R
JournalParasitology
PubMed ID12211609
'The direct effects of phytohormones (auxin and kinetin) and root diffusates on the surface lipophilicity of the plant parasitic nematodes Globodera rostochiensis and Meloidogyne incognita were investigated. The fluorescent lipid probe AF18 (5-N(octodecanoyl) aminofluorescein) was used to detect surface changes. Root diffusates increased AF18 uptake by G. rostochiensis while it ... More
The mechanism of action of GTP on Ca2+ efflux from rat liver microsomal vesicles. Measurement of vesicle fusion by fluorescence energy transfer.
AuthorsComerford JG, Dawson AP
JournalBiochem J
PubMed ID3342018
'1. GTP-promoted fusion between microsomal vesicles was studied by using fluorescence-resonance-energy transfer between the fluorescent membrane probes octadecanoyl-aminofluorescein and octadecyl-rhodamine. 2. The fluorescence increase after GTP addition does not require the presence of ATP, is unaffected by changes in free [Ca2+] in the range 10 microM-1 nM, but requires Mg2+, ... More
Developmental changes in the lateral diffusion of Leydig cell membranes measured by the FRAP method.
AuthorsZlatanov IV, Foley M, Birmingham J, Garland PB
JournalFEBS Lett
PubMed ID3653400
A simple method for isolation and fluorescence labelling of Leydig cells (L-cells) from rat testes was developed. Lateral diffusion coefficients of both lipid and protein membrane fluorescent probes were measured by the method of fluorescence recovery after photobleaching (FRAP). Age-dependent changes in diffusibility of membrane lipids and proteins were discovered. ... More
Lipid lateral diffusion and membrane organization.
AuthorsTocanne JF, Dupou-Cézanne L, Lopez A, Tournier JF
JournalFEBS Lett
PubMed ID2680602
It is shown that investigating the lateral motion of lipids in biological membranes can provide useful information on membrane lateral organization. After labeling membranes with extrinsic or intrinsic lipophilic fluorescent probes, fluorescence recovery after photobleaching experiments strongly suggests that specialized cells like spermatozoa, eggs and epithelia exhibit surface membrane regionalization ... More
Cheek cell membrane fluidity measured by fluorescence recovery after photobleaching and steady-state fluorescence anisotropy.
AuthorsLadha S, Mackie AR, Clark DC
JournalJ Membr Biol
PubMed ID7884814
Membrane fluidity of human cheek cells was determined using fluorescence recovery after photobleaching (FRAP) and steady-state fluorescence anisotropy. The FRAP data showed that the lateral diffusion coefficient (D) and mobile fraction (%R) of lipid in the plasma membrane of control cells were 2.01 x 10(-9) cm2/sec and 54.25%, respectively. Trypsin ... More
The lateral diffusion of lipid probes in the surface membrane of Schistosoma mansoni.
AuthorsFoley M, MacGregor AN, Kusel JR, Garland PB, Downie T, Moore I
JournalJ Cell Biol
PubMed ID3745270
The technique of fluorescence recovery after photobleaching was used to measure the lateral diffusion of fluorescent lipid analogues in the surface membrane of Schistosoma mansoni. Our data reveal that although some lipids could diffuse freely others exhibited restricted lateral diffusion. Quenching of lipid fluorescence by a non-permeant quencher, trypan blue, ... More
Real-time measurements of chemically-induced membrane fusion in cell monolayers, using a resonance energy transfer method.
AuthorsPartearroyo MA, Cabezón E, Nieva JL, Alonso A, Goñi FM
JournalBiochim Biophys Acta
PubMed ID8292622
Fusion of mouse melanoma cells grown in monolayers has been directly monitored by fluorescence resonance energy transfer between fluorescein and rhodamine probes attached to octadecanoic acid. Various poly(ethylene glycol)s (PEG), either alone or in combination with amphipathic molecules, have been used as fusogens. Fusion starts at a maximum rate as ... More
Studies of chemotactic factor-induced polarity in human neutrophils. Lipid mobility, receptor distribution and the time-sequence of polarization.
AuthorsMcKay DA, Kusel JR, Wilkinson PC
JournalJ Cell Sci
PubMed ID1808201
Differences in membrane composition between the anterior and posterior poles of human blood neutrophils on exposure to chemoattractant were studied using a laser microscope, and the effects of attractant concentration and time on the ability of neutrophils to polarize were determined. The findings were as follows. (1) The fluorescein-labelled chemotactic ... More
The DvA-1 polyprotein of the parasitic nematode Dictyocaulus viviparus. A small helix-rich lipid-binding protein.
AuthorsKennedy MW, Britton C, Price NC, Kelly SM, Cooper A
JournalJ Biol Chem
PubMed ID7642601
DNA encoding a single unit of the DvA-1 polyprotein of the parasitic nematode Dictyocaulus viviparus was isolated and the polypeptide ("rDvA-1L") expressed in Escherichia coli, to give a protein showing high binding affinity for fatty acids and retinoids. Fluorescent fatty acid probes show substantial changes in emission spectrum in the ... More
Epstein-Barr virus enters B cells and epithelial cells by different routes.
AuthorsMiller N, Hutt-Fletcher LM
JournalJ Virol
PubMed ID1316456
Epstein-Barr virus (EBV) infects two cell types, B lymphocytes and epithelial cells. Electron microscopic studies have shown that the virus fuses with the lymphoblastoid cell line Raji but is endocytosed into thin-walled non-clathrin-coated vesicles in normal B cells before fusion takes place. To compare early interactions of EBV with epithelial ... More
Molecular mobility in the monolayers of foam films stabilized by porcine lung surfactant.
AuthorsLalchev ZI, Todorov RK, Christova YT, Wilde PJ, Mackie AR, Clark DC
JournalBiophys J
PubMed ID8913597
Certain physical properties of a range of foam film types that are believed to exist in vivo in the lung have been investigated. The contribution of different lung surfactant components found in porcine lung surfactant to molecular surface diffusion in the plane of foam films has been investigated for the ... More
Lipid domains of mycobacteria studied with fluorescent molecular probes.
AuthorsChristensen H, Garton NJ, Horobin RW, Minnikin DE, Barer MR
JournalMol Microbiol
PubMed ID10200973
The complex mycobacterial cell envelope is recognized as a critical factor in our failure to control tuberculosis, leprosy and other non-tuberculous pathogens. Although its composition has been extensively determined, many details regarding the organization of the envelope remain uncertain. This is particularly so for the non-covalently bound lipids, whose natural ... More