Cytosolic delivery of membrane-impermeable molecules in dendritic cells using pH-responsive core-shell nanoparticles.
AuthorsHu Y, Litwin T, Nagaraja AR, Kwong B, Katz J, Watson N, Irvine DJ,
JournalNano Lett
PubMed ID17887715
'Polycations that absorb protons in response to the acidification of endosomes can theoretically disrupt these vesicles via the "proton sponge" effect. To exploit this mechanism, we created nanoparticles with a segregated core-shell structure for efficient, noncytotoxic intracellular drug delivery. Cross-linked polymer nanoparticles were synthesized with a pH-responsive core and hydrophilic ... More
In vivo targeting of dendritic cells for activation of cellular immunity using vaccine carriers based on pH-responsive microparticles.
AuthorsKwon YJ, James E, Shastri N, Fréchet JM
JournalProc Natl Acad Sci U S A
PubMed ID16344458
'Activating the immune system to trigger a specific response is a major challenge in vaccine development. In particular, activating sufficient cytotoxic T lymphocyte-mediated cellular immunity, which is crucial for the treatment of many diseases including cancer and AIDS, has proven to be especially challenging. In this study, antigens were encapsulated ... More
Distinct pathways of antigen uptake and intracellular routing in CD4 and CD8 T cell activation.
AuthorsBurgdorf S, Kautz A, Böhnert V, Knolle PA, Kurts C
JournalScience
PubMed ID17463291
The mechanisms that allow antigen-presenting cells (APCs) to selectively present extracellular antigen to CD8+ effector T cells (cross-presentation) or to CD4+ T helper cells are not fully resolved. We demonstrated that APCs use distinct endocytosis mechanisms to simultaneously introduce soluble antigen into separate intracellular compartments, which were dedicated to presentation ... More
A microengraving method for rapid selection of single cells producing antigen-specific antibodies.
AuthorsLove JC, Ronan JL, Grotenbreg GM, van der Veen AG, Ploegh HL
JournalNat Biotechnol
PubMed ID16699501
Monoclonal antibodies that recognize specific antigens of interest are used as therapeutic agents and as tools for biomedical research. Discovering a single monoclonal antibody requires retrieval of an individual hybridoma from polyclonal mixtures of cells producing antibodies with a variety of specificities. The time required to isolate hybridomas by a ... More
Early innate and adaptive immune perturbations determine long-term severity of chronic virus and Mycobacterium tuberculosis coinfection.
Authors
JournalImmunity
PubMed ID33515487
Profiling protein expression in circulating tumour cells using microfluidic western blotting.
Authors
JournalNat Commun
PubMed ID28332571
MHC Class II Antigen Presentation by Lymphatic Endothelial Cells in Tumors Promotes Intratumoral Regulatory T cell-Suppressive Functions.
Authors
JournalCancer Immunol Res
PubMed ID33952631
Lymph Node Stromal Cells Generate Antigen-Specific Regulatory T Cells and Control Autoreactive T and B Cell Responses.
Authors
JournalCell Rep
PubMed ID32209472
Inflammasomes within Hyperactive Murine Dendritic Cells Stimulate Long-Lived T Cell-Mediated Anti-tumor Immunity.