Lifetime-based pH sensors: indicators for acidic environments.
AuthorsLin HJ, Szmacinski H, Lakowicz JR
JournalAnal Biochem
PubMed ID10094788
'We characterized the pH-dependent intensity decays of three fluorophores, Oregon green 514 carboxylic acid, Cl-NERF, and DM-NERF, using frequency-domain fluorometry, with the objective of identifying lifetime-based sensors for low pH values. These three probes were originally designed as dual excitation wavelength-ratiometric probes, with high photostability and high quantum yields in ... More
Synthesis and fluorescence properties of Oregon Green 514 labeled peptides.
AuthorsDelmotte C, Delmas A
JournalBioorg Med Chem Lett
PubMed ID10571161
Oregon Green 514, with high photostability and a pKa of 4.8, is suitable for imaging applications. Labeling of peptide-bound resin with the carboxylic acid form was optimized. Peptide conjugation resulted in altered fluorescence properties of the dye including a quenching of the intensity compared with that in aqueous buffers with ... More