Real-time multiple particle tracking of gene nanocarriers in complex biological environments.
AuthorsLai SK, Hanes J,
JournalMethods Mol Biol
PubMed ID18470640
'Complex biological fluids, such as the vast and molecularly crowded cell cytoplasm and the highly viscoelastic mucus that protects many entry ways to the body, pose significant barriers to efficient gene delivery. Understanding the dynamics of gene carriers in such environments allows insight that leads to rational improvements in gene ... More
A mismatch-selective bifunctional rhodium-Oregon Green conjugate: a fluorescent probe for mismatched DNA.
AuthorsZeglis BM, Barton JK,
JournalJ Am Chem Soc
PubMed ID16637630
A mismatch-selective bifunctional rhodium-Oregon Green conjugate: a fluorescent probe for mismatched DNA. Zeglis BM, Barton JK. Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA. Publication Types: Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. PMID: 16637630 [PubMed - indexed for MEDLINE] ... More
Synthesis and characterization of fluorescent cobalamin (CobalaFluor) derivatives for imaging.
Fluorescent derivatives of cobalamin have been prepared by linking fluorophores to cobalamin through a propylamide spacer. Fluorescein, naphthofluorescein, and Oregon Green derivatives have been prepared in good yield by reaction of the fluorophore NHS-ester with beta-(3-aminopropyl)cobalamin to form fluorescent cobalamin conjugates (CobalaFluors) that are potentially suitable for the in vitro ... More
Fiber-optic nitric oxide-selective biosensors and nanosensors.
AuthorsBarker SL, Kopelman R, Meyer TE, Cusanovich MA
JournalAnal Chem
PubMed ID9511472
Fiber-optic biosensors that are selective for nitric oxide and do not respond to most potential interferents have been prepared with cytochromes c'. Both micro- and nanosensors have been prepared, and their response is fast (< 1 s), reversible, and linear up to 1 mM nitric oxide. The detection limit is ... More
Novel immuno-FRET assay method for Bacillus spores and Escherichia coli O157:H7.
AuthorsBruno JG, Ulvick SJ, Uzzell GL, Tabb JS, Valdes ER, Batt CA
JournalBiochem Biophys Res Commun
PubMed ID11573946
Novel immunofluorescence resonance energy transfer (immuno-FRET) assays for both Bacillus cereus spores and Escherichia coli O157:H7 are reported. Both assays involve the use of dual (QSY-7 and Oregon Green 514-antibody)-labeled spores or vegetative bacteria, such that Oregon Green 514-labeled antibodies are quenched by proximal QSY-7 molecules that are covalently bound ... More
Alexa and Oregon Green dyes as fluorescence anisotropy probes for measuring protein-protein and protein-nucleic acid interactions.
AuthorsRusinova E, Tretyachenko-Ladokhina V, Vele OE, Senear DF, Alexander Ross JB
JournalAnal Biochem
PubMed ID12234459
The fluorescence properties of Alexa 488, Oregon Green 488, and Oregon Green 514 (Molecular Probes (Eugene, OR)) are compared when conjugated to biomolecules and as model compounds free in solution. We show that these relatively new, green fluorescence probes are excellent probes for investigation of the thermodynamics of protein-protein and ... More
Leakage responses to L-NAME differ with the fluorescent dye used to label albumin.
AuthorsRumbaut RE, Harris NR, Sial AJ, Huxley VH, Granger DN
JournalAm J Physiol
PubMed ID9887048
Nitric oxide synthase (NOS) inhibitors have been reported to increase as well as to decrease microvascular transport of macromolecules in a variety of models. This study was performed to determine whether the influence of NOS inhibition on albumin leakage was dependent on the fluorescent dyes used to label albumin. Albumin ... More
Real-time imaging of fluorescent flagellar filaments.
AuthorsTurner L, Ryu WS, Berg HC
JournalJ Bacteriol
PubMed ID10781548
Bacteria swim by rotating flagellar filaments that are several micrometers long, but only about 20 nm in diameter. The filaments can exist in different polymorphic forms, having distinct values of curvature and twist. Rotation rates are on the order of 100 Hz. In the past, the motion of individual filaments ... More
Sensitivity enhancement in fluorescence correlation spectroscopy of multiple species using time-gated detection.
AuthorsLamb DC, Schenk A, Röcker C, Scalfi-Happ C, Nienhaus GU
JournalBiophys J
PubMed ID10920042
Fluorescence correlation spectroscopy (FCS) is a powerful technique to measure chemical reaction rates and diffusion coefficients of molecules in thermal equilibrium. The capabilities of FCS can be enhanced by measuring the energy, polarization, or delay time between absorption and emission of the collected fluorescence photons in addition to their arrival ... More
Characterization of visible dyes for four-decay fluorescence detection in DNA sequencing.
AuthorsNunnally BK, He H, Li LC, Tucker SA, McGown LB
JournalAnal Chem
PubMed ID9212706
Dyes of several classes were investigated as candidates for use in a multiplex, four-decay fluorescence detection scheme for DNA sequencing. The dyes include nitrobenzofuran dyes, rhodamine dyes, fluorescein dyes, cyanine dyes, Nile Red, and BODIPY dyes. Based on the results of fluorescence spectral and lifetime studies, an initial set of ... More
Presence and phosphorylation of transcription factors in developing dendrites.
In screening amplified poly(A) mRNA from hippocampal dendrites and growth cones in culture to determine candidates for local translation, we found that select transcription factor mRNAs were present. We hypothesized that synthesis of transcription factor proteins within dendrites would provide a direct signaling pathway between the distal dendrite and the ... More
Role of COPI in phagosome maturation.
AuthorsBotelho RJ, Hackam DJ, Schreiber AD, Grinstein S
JournalJ Biol Chem
PubMed ID10748188
Phagosomes mature by sequentially fusing with endosomes and lysosomes. Vesicle budding is presumed to occur concomitantly, mediating the retrieval of plasmalemmal components and the regulation of phagosomal size. We analyzed whether fission of vesicles from phagosomes requires COPI, a multimeric complex known to be involved in budding from the Golgi ... More