Insect lipoprotein follows a transferrin-like recycling pathway that is mediated by the insect LDL receptor homologue.
AuthorsVan Hoof D, Rodenburg KW, Van der Horst DJ
JournalJ Cell Sci
PubMed ID12356906
'The lipoprotein of insects, high-density lipophorin (HDLp), is homologous to that of mammalian low-density lipoprotein (LDL) with respect to its apolipoprotein structure. Moreover, an endocytic receptor for HDLp has been identified (insect lipophorin receptor, iLR) that is homologus to the LDL receptor. We transfected LDL-receptor-expressing CHO cells with iLR cDNA ... More
Motile tubular vacuoles in extramatrical mycelium and sheath hyphae of ectomycorrhizal systems.
AuthorsAllaway WG, Ashford AE
JournalProtoplasma
PubMed ID11732061
'Extramatrical mycelium and outer hyphae of the sheath of Eucalyptus pilularis-Pisolithus tinctorius mycorrhizas contain abundant motile tubular vacuoles which accumulate the carboxyfluorescein analogue Oregon Green 488 carboxylic acid. The fluorochrome accumulates in a system of small vacuoles, tubules, and larger vacuoles, which are interlinked, motile, and pleiomorphic, in external hyphae, ... More
Visualisation of the uptake of two model xenobiotics into bean leaves by confocal laser scanning microscopy: diffusion pathways and implication in phloem translocation.
AuthorsLiu Z, Gaskin RE
JournalPest Manag Sci
PubMed ID15154509
'The diffusion of two fluorescent dyes, Oregon Green 488 (Oregon Green) and Rhodamine B into the leaves of broad bean (Vicia faba L) plants was studied to simulate the foliar uptake process of pesticides. The uptake rate of these model xenobiotics into bean foliage was measured using a standard leaf ... More
Fast electrical lysis of cells for capillary electrophoresis.
AuthorsHan F, Wang Y, Sims CE, Bachman M, Chang R, Li GP, Allbritton NL
JournalAnal Chem
PubMed ID14572031
'In the past decade, capillary electrophoresis has demonstrated increasing utility for the quantitative analysis of single cells. New applications for the analysis of dynamic cellular properties demand sampling methods with sufficient temporal resolution to accurately measure these processes. In particular, intracellular signaling pathways involving many enzymes can be modulated on ... More
Fluorescent indicators for intracellular pH.
AuthorsHan J, Burgess K,
JournalChem Rev
PubMed ID19831417
This review is about intracellular pH sensors, includingsmall fluorescent organic molecules, nanoparticles, andfluorescent proteins, e.g., GFP. It focuses on their preparations, photophysical properties, and advantages/disadvantagesfor intracellular pH measurements. The discussion is limitedto fluorescent indicators that have been applied to measureintracellular pH values since 1980. ... More
Comparative photostability studies of BODIPY and fluorescein dyes by using fluorescence correlation spectroscopy.
AuthorsHinkeldey B, Schmitt A, Jung G,
JournalChemphyschem
PubMed ID18816535
In single-molecule applications, the photostability of fluorescent molecules is a key parameter. We apply fluorescence correlation spectroscopy to compare the photostability of four fluorescein and four borondipyrromethene (BODIPY) dyes of similar structure but different triplet yields. The latter class of dyes are more stable. In the kinetic analysis the, diffusion ... More
Penetration and distribution of three lipophilic probes in vitro in human skin focusing on the hair follicle.
AuthorsGrams YY, Bouwstra JA
JournalJ Control Release
PubMed ID12363451
Fluorescent model substances of increasing lipophilicity (Oregon Green) 488, Bodipy, FL C5 and Bodipy 564/570 C5) were selected to enable the visualization in the skin using confocal laser scanning microscopy. After measuring the penetration for 18 h, the nonfixed human scalp skin was imaged from the bottom parallel to the ... More
Permeant lipophilicity and vehicle composition influence accumulation of dyes in hair follicles of human skin.
In skin and hair research drug targeting to the hair follicle is of great interest. Therefore the influence of permeant lipophilicity and vehicle composition on local accumulation has been examined using confocal laser scanning microscopy (CLSM). Formulations saturated with either Oregon Green 488, Bodipy FL C(5) or Bodipy 564/570 C(5) ... More