The kinetics of aminosidine in renal patients with different degrees of renal failure.
AuthorsNovarini A, Montanari A, Bruschi G, Rossi E, Borghetti A, Migone L
JournalClin Nephrol
PubMed ID1157346
'The plasma concentration and urinary excretion after a single 500 mg dose of Aminosidine have been studied in 12 patients with different degrees of renal failure and 4 normal subjects. In normal subjects the plasma half-life is 2.47 hr; in patients with creatinine clearance (Ccr) of 30-60 ml/min, its 6.7 ... More
Novel immuno-FRET assay method for Bacillus spores and Escherichia coli O157:H7.
AuthorsBruno JG, Ulvick SJ, Uzzell GL, Tabb JS, Valdes ER, Batt CA
JournalBiochem Biophys Res Commun
PubMed ID11573946
Novel immunofluorescence resonance energy transfer (immuno-FRET) assays for both Bacillus cereus spores and Escherichia coli O157:H7 are reported. Both assays involve the use of dual (QSY-7 and Oregon Green 514-antibody)-labeled spores or vegetative bacteria, such that Oregon Green 514-labeled antibodies are quenched by proximal QSY-7 molecules that are covalently bound ... More
Peripheral blood-derived CD34+ progenitor cells: CXC chemokine receptor 4 and CC chemokine receptor 5 expression and infection by HIV.
AuthorsRuiz ME, Cicala C, Arthos J, Kinter A, Catanzaro AT, Adelsberger J, Holmes KL, Cohen OJ, Fauci AS
JournalJ Immunol
PubMed ID9780190
The present study demonstrates cell surface expression of both CXC chemokine receptor 4 (CXCR4) and CC chemokine receptor 5 (CCR5), major coreceptors for T cell-tropic and macrophage-tropic strains of HIV, respectively, on CD34+ progenitor cells derived from the peripheral blood. CD34+ progenitor cells were susceptible to infection by diverse strains ... More
Herp, a new ubiquitin-like membrane protein induced by endoplasmic reticulum stress.
AuthorsKokame K, Agarwala KL, Kato H, Miyata T
JournalJ Biol Chem
PubMed ID10922362
Hyperhomocysteinemia, a risk factor for vascular disease, injures endothelial cells through undefined mechanisms. We previously identified several homocysteine-responsive genes in cultured human vascular endothelial cells, including the endoplasmic reticulum (ER)-resident molecular chaperone GRP78/BiP. Here, we demonstrate that homocysteine induces the ER stress response and leads to the expression of a ... More
Class VI unconventional myosin is required for spermatogenesis in Drosophila.
AuthorsHicks JL, Deng WM, Rogat AD, Miller KG, Bownes M
JournalMol Biol Cell
PubMed ID10588662
We have identified partial loss of function mutations in class VI unconventional myosin, 95F myosin, which results in male sterility. During spermatogenesis the germ line precursor cells undergo mitosis and meiosis to form a bundle of 64 spermatids. The spermatids remain interconnected by cytoplasmic bridges until individualization. The process of ... More