Oregon Green™ 488 BAPTA-1 葡聚糖,钾盐,10,000 MW,阴离子
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Oregon Green™ 488 BAPTA-1 葡聚糖,钾盐,10,000 MW,阴离子
Invitrogen™

Oregon Green™ 488 BAPTA-1 葡聚糖,钾盐,10,000 MW,阴离子

标记钙指示剂是结合 Ca2+ 离子后荧光强度增加的分子。其可用于多种钙信号转导研究,包括测量细胞内 Ca2+、跟踪 Ca2+ 进入和释放以及对活组织中 Ca2+ 进行多光子激发成像了解更多信息
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货号数量
O67985 mg
货号 O6798
价格(CNY)
6,024.00
飞享价
Ends: 31-Dec-2025
7,901.00
共减 1,877.00 (24%)
Each
添加至购物车
数量:
5 mg
价格(CNY)
6,024.00
飞享价
Ends: 31-Dec-2025
7,901.00
共减 1,877.00 (24%)
Each
添加至购物车
标记钙指示剂是结合 Ca2+ 离子后荧光强度增加的分子。其可用于多种钙信号转导研究,包括测量细胞内 Ca2+、跟踪 Ca2+ 进入和释放以及对活组织中 Ca2+ 进行多光子激发成像。可以使用膜片移液管、显微注射或我们的 Influx 胞饮上样试剂,以物理方式将细胞不可透过性盐形式的这些葡聚糖偶联指示剂上样至细胞中。利用荧光显微镜检查测量这些细胞的荧光信号。与 AM 酯形式相比,我们的葡聚糖形式的钙指示剂显示泄漏和区室化出现显著下降。

了解有关钙离子、钾离子、pH 和膜电位指示剂等离子指示剂更多信息 ›

钙指示剂(细胞不可透过性盐,葡聚糖偶联物)规格:
• 标记(激发/发射波长):Oregon Green™ 488 BAPTA-1 (494/523 nm)
• 结合 Ca2+ 后荧光强度增加:∼14 倍
•葡聚糖规格:10,000 MW
• 结合 Ca2+ 后显示荧光增强,波长变化较小


Molecular Probes 的光谱特性™ 钙指示剂
这些探针被可见光激发,并且由于激发所需能量低,细胞光损伤电位降低。常用的激光仪器(如共聚焦激光扫描显微镜)能够有效激发这类指示剂,且其发射光谱位于通常不受细胞自发荧光和散射背景干扰的光谱区域。

更丰富的钙荧光指示剂的选择
我们提供了大量 Molecular Probes™ 钙指示剂,可用于各种实验方案,例如右旋糖酐形式可减少泄漏和区室化、 BAPTA 偶联物用于检测高幅钙瞬变。更多信息请参阅 Molecular Probes™ 手册可见光激发的 Ca2+ 荧光指示剂—第 19.3 节

对于 UV 激发的 Ca2+ 指示剂、基于蛋白的 Ca2+ 指示剂、Ca2+ 指示剂的偶联物以及其他金属离子(即 Mg2+、Zn2+)的荧光指示剂,请查看 Molecular Probes™ 手册中的 Ca2+、Mg2+、Zn2+ 以及其他金属离子指示剂—第 19 章

仅供科研使用。不适用于动物或人类的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型基于荧光染料
数量5 mg
运输条件室温
适用于(应用)钾含量测定试剂盒
适用于(设备)荧光显微镜, 流式细胞仪, 微孔板读数仪
产品线Oregon Green
产品类型试剂名称
Unit SizeEach
内容与储存
在冷冻冰箱(-5°C 至 -30°C)中避光储存。

引用和文献 (37)

引用和文献
Abstract
Spatially organised mitochondrial calcium uptake through a novel pathway in chick neurones.
Authors:Coatesworth W, Bolsover S
Journal:Cell Calcium
PubMed ID:16338004
'A brief depolarisation of chick sensory neurones evokes a calcium increase in mitochondria that peaks 1-2s after the depolarisation event and then decays over tens of seconds. Peripheral mitochondria take up more calcium than do central ones, even when the cytosolic calcium increase is spatially homogeneous. The calcium influx into ... More
The sources and sequestration of Ca(2+) contributing to neuroeffector Ca(2+) transients in the mouse vas deferens.
Authors:Brain KL, Cuprian AM, Williams DJ, Cunnane TC
Journal:J Physiol
PubMed ID:14500773
'The detection of focal Ca(2+) transients (called neuroeffector Ca(2+) transients, or NCTs) in smooth muscle of the mouse isolated vas deferens has been used to detect the packeted release of ATP from nerve terminal varicosities acting at postjunctional P2X receptors. The present study investigates the sources and sequestration of Ca(2+) ... More
Control of IP(3)-mediated Ca2+ puffs in Xenopus laevis oocytes by the Ca2+-binding protein parvalbumin.
Authors:John LM, Mosquera-Caro M, Camacho P, Lechleiter JD
Journal:J Physiol
PubMed ID:11507154
'1. Elementary events of Ca2+ release (Ca2+ puffs) can be elicited from discrete clusters of inositol 1,4,5 trisphosphate receptors (IP(3)Rs) at low concentrations of IP(3). Ca(2+) puffs have rarely been observed unless elicited by either hormone treatment or introduction of IP(3) into the cell. However, cells appear to have sufficient ... More
Calcium in sympathetic varicosities of mouse vas deferens during facilitation, augmentation and autoinhibition.
Authors:Brain KL, Bennett MR
Journal:J Physiol
PubMed ID:9279805
'1. The sympathetic nerve terminals of the mouse vas deferens were loaded with the calcium indicator Oregon Green 488 BAPTA-1 by orthograde transport along the postganglionic nerves. Changes in the calcium concentration in the varicosity (delta [Ca2+]v) were determined following single impulses, and short (5-impulse) and long (200-impulse) trains at ... More
Coupling of calcium homeostasis to axonal sodium in axons of mouse optic nerve.
Authors:Verbny Y, Zhang CL, Chiu SY
Journal:J Neurophysiol
PubMed ID:12163532
'Axonal populations in neonatal and mature optic nerves were selectively stained with calcium dyes for analysis of calcium homeostasis and its possible coupling to axonal Na. Repetitive nerve stimulation causes a rise in axonal [Ca(2+)](i) the posttetanus recovery of which is impeded by increasing the number of action potentials in ... More