Oregon Green™ 488 BAPTA-5N,六钾盐,细胞不可透过性
Oregon Green™ 488 BAPTA-5N,六钾盐,细胞不可透过性
Invitrogen™

Oregon Green™ 488 BAPTA-5N,六钾盐,细胞不可透过性

不可渗透细胞的可见光可激发、低亲和力 Ca2+ 指示剂 Oregon Green™ 488 BAPTA-5N 六钾盐对 Ca2+了解更多信息
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货号数量
O6812500 μg
货号 O6812
价格(CNY)
4,874.00
Each
添加至购物车
数量:
500 μg
价格(CNY)
4,874.00
Each
添加至购物车
不可渗透细胞的可见光可激发、低亲和力 Ca2+ 指示剂

Oregon Green™ 488 BAPTA-5N 六钾盐

对 Ca2+ 的 Kd 为 ∼20 µM,最大吸收/发射波长为 ∼492/517 nm。

了解有关离子指示剂(包括钙、钾、pH 值和膜电位指示剂)的更多信息›
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型基于荧光染料
数量500 μg
运输条件室温
适用于(设备)荧光显微镜, 流式细胞仪, 微孔板读数仪
产品线Oregon Green
产品类型钙指示剂
Unit SizeEach
内容与储存
在冷冻冰箱(-5°C 至 -30°C)中避光储存。

引用和文献 (46)

引用和文献
Abstract
Presynaptic Ca(2+) influx at the inhibitor of the crayfish neuromuscular junction: a photometric study at a high time resolution.
Authors:Vyshedskiy A, Lin JW
Journal:J Neurophysiol
PubMed ID:10634895
'Presynaptic calcium influx at the inhibitor of the crayfish neuromuscular junction was investigated by measuring fluorescence transients generated by calcium-sensitive dyes. This approach allowed us to correlate presynaptic calcium influx with transmitter release at a high time resolution. Systematic testing of the calcium indicators showed that only low-affinity dyes, with ... More
Light dependence of calcium and membrane potential measured in blowfly photoreceptors in vivo.
Authors:Oberwinkler J, Stavenga DG
Journal:J Gen Physiol
PubMed ID:9689022
'Light adaptation in insect photoreceptors is caused by an increase in the cytosolic Ca2+ concentration. To better understand this process, we measured the cytosolic Ca2+ concentration in vivo as a function of adapting light intensity in the white-eyed blowfly mutant chalky. We developed a technique to measure the cytosolic Ca2+ ... More
Stimulation-evoked increases in cytosolic [Ca(2+)] in mouse motor nerve terminals are limited by mitochondrial uptake and are temperature-dependent.
Authors:David G, Barrett EF
Journal:J Neurosci
PubMed ID:11007886
'Increases in cytosolic [Ca(2+)] evoked by trains of action potentials (20-100 Hz) were recorded from mouse and lizard motor nerve terminals filled with a low-affinity fluorescent indicator, Oregon Green BAPTA 5N. In mouse terminals at near-physiological temperatures (30-38 degrees C), trains of action potentials at 25-100 Hz elicited increases in ... More
Direct measurement of SR release flux by tracking 'Ca2+ spikes' in rat cardiac myocytes.
Authors:Song LS, Sham JS, Stern MD, Lakatta EG, Cheng H
Journal:J Physiol
PubMed ID:9769413
'1. Ca2+ release flux across the sarcoplasmic reticulum (SR) during cardiac excitation-contraction coupling was investigated using a novel fluorescence method. Under whole-cell voltage-clamp conditions, rat ventricular myocytes were dialysed with a high concentration of EGTA (4.0 mM, 150 nM free Ca2+), to minimize the residence time of released Ca2+ in ... More
Activation of trypsinogen in large endocytic vacuoles of pancreatic acinar cells.
Authors:Sherwood MW, Prior IA, Voronina SG, Barrow SL, Woodsmith JD, Gerasimenko OV, Petersen OH, Tepikin AV
Journal:Proc Natl Acad Sci U S A
PubMed ID:17363470
'The intracellular activation of trypsinogen, which is both pH- and calcium-dependent, is an important early step in the development of acute pancreatitis. The cellular compartment in which trypsinogen activation occurs currently is unknown. We therefore investigated the site of intracellular trypsinogen activation by using an established cellular model of acute ... More