The Ste20 kinases Ste20-related proline-alanine-rich kinase and oxidative-stress response 1 regulate NKCC1 function in sensory neurons.
AuthorsGeng Y, Hoke A, Delpire E,
JournalJ Biol Chem
PubMed ID19307180
'NKCC1 is highly expressed in dorsal root ganglion neurons, where it is involved in gating sensory information. In a recent study, it was shown that peripheral nerve injury results in increased NKCC1 activity, not due to an increase in cotransporter expression, but to increased phosphorylation of the cotransporter (Pieraut, S., ... More
A novel initiation mechanism of death in Streptococcus pneumoniae induced by the human milk protein-lipid complex HAMLET and activated during physiological death.
AuthorsClementi EA, Marks LR, Duffey ME, Hakansson AP,
JournalJ Biol Chem
PubMed ID22700972
'To cause colonization or infection, most bacteria grow in biofilms where differentiation and death of subpopulations is critical for optimal survival of the whole population. However, little is known about initiation of bacterial death under physiological conditions. Membrane depolarization has been suggested, but never shown to be involved, due to ... More
Osteoclast response to low extracellular sodium and the mechanism of hyponatremia-induced bone loss.
AuthorsBarsony J, Sugimura Y, Verbalis JG,
JournalJ Biol Chem
PubMed ID21135109
'Our recent animal and human studies revealed that chronic hyponatremia is a previously unrecognized cause of osteoporosis that is associated with increased osteoclast numbers in a rat model of the human disease of the syndrome of inappropriate antidiuretic hormone secretion (SIADH). We used cellular and molecular approaches to demonstrate that ... More
Receptor-selective diffusion barrier enhances sensitivity of astrocytic processes to metabotropic glutamate receptor stimulation.
AuthorsArizono M, Bannai H, Nakamura K, Niwa F, Enomoto M, Matsu-Ura T, Miyamoto A, Sherwood MW, Nakamura T, Mikoshiba K,
JournalSci Signal
PubMed ID22472649
Metabotropic glutamate receptor (mGluR)-dependent calcium ion (Ca²+) signaling in astrocytic processes regulates synaptic transmission and local blood flow essential for brain function. However, because of difficulties in imaging astrocytic processes, the subcellular spatial organization of mGluR-dependent Ca²+ signaling is not well characterized and its regulatory mechanism remains unclear. Using genetically ... More
Transient Ca2+ mobilization caused by osmotic shock initiates salmonid fish sperm motility.
AuthorsTakei GL, Mukai C, Okuno M,
JournalJ Exp Biol
PubMed ID22279070
Salmonid fish sperm motility is known to be suppressed in millimolar concentrations of extracellular K(+), and dilution of K(+) upon spawning triggers cAMP-dependent signaling for motility initiation. In a previous study, however, we demonstrated that suspending sperm in a 10% glycerol solution and subsequent dilution into a low-osmotic solution induced ... More
Genetic divergence of rotavirus nonstructural protein 4 results in distinct serogroup-specific viroporin activity and intracellular punctate structure morphologies.
AuthorsHyser JM, Utama B, Crawford SE, Estes MK,
JournalJ Virol
PubMed ID22357281
Nonstructural protein 4 (NSP4) viroporin activity is critical for the replication and assembly of serogroup A rotavirus (RVA); however, the dramatic primary sequence divergence of NSP4s across serogroups raises the possibility that viroporin activity is not a common feature among RVs. We tested for NSP4 viroporin activity from divergent strains, ... More
Cell-based potassium ion channel screening using the FluxOR assay.
AuthorsBeacham DW, Blackmer T, O' Grady M, Hanson GT,
JournalJ Biomol Screen
PubMed ID20208034
FluxOR technology is a cell-based assay used for high-throughput screening measurements of potassium channel activity. Using thallium influx as a surrogate indicator of potassium ion channel activity, the FluxOR Potassium Ion Channel Assay is based on the activation of a novel fluorescent dye. This indicator reports channel activity with a ... More
Novel metabolic role for BDNF in pancreatic β-cell insulin secretion.
Authors
JournalNat Commun
PubMed ID32327658
Plasma magnesium is inversely associated with Epstein-Barr virus load in peripheral blood and Burkitt lymphoma in Uganda.