Fluorescent and photoactivatable fluorescent derivatives of tetrodotoxin to probe the sodium channel of excitable membranes.
AuthorsAngelides KJ
JournalBiochemistry
PubMed ID6269580
'Fluorescent and photoactivatable fluorescent derivatives of tetrodotoxin (TTX) have been synthesized. N-Methylanthraniloylglycine hydrazide, anthraniloyl hydrazide, and 2-azidoanthraniloylglycine hydrazide were coupled to the carbonyl at C6 of oxidized tetrodotoxin to form stable fluorescent hydrazones. The C6 ketone can be reductively aminated with either ammonium or methylammonium acetate to form 6-amino- or ... More
Acanthocytosis and cholesterol enrichment decrease lipid fluidity of only the outer human erythrocyte membrane leaflet.
AuthorsFlamm M, Schachter D
JournalNature
PubMed ID6896363
'The structure and functions of the human erythrocyte are influenced by the composition and organization of the membrane lipids. The outer (exofacial) and inner (endofacial) leaflets of the erythrocyte membrane differ in lipid composition, and recent studies using a group of membrane-impermeant pyrene fluorophores have demonstrated that the lipid fluidity ... More
Labeling of the glycoprotein subunit of (Na,K)ATPase with fluorescent probes.
AuthorsLee JA, Fortes PA
JournalBiochemistry
PubMed ID2983755
'Sodium plus potassium activated adenosinetriphosphatase [(Na,K)ATPase] is composed of a catalytic subunit (alpha) and a glycoprotein subunit (beta) of unknown function. A method has been developed to label the beta subunit of purified dog kidney (Na,K)ATPase with fluorescent probes. The method consists of oxidation of beta-subunit oligosaccharides, reaction of the ... More
Pyrene derivatives as fluorescent probes of conformation near the 3' termini of polyribonucleotides.
AuthorsKoenig P, Reines SA, Cantor CR
JournalBiopolymers
PubMed ID911999
Fluorescent-labeled crosslinks in collagen: pyrenebutyrylhydrazine.
AuthorsShambaugh N, Fujimori E
JournalBiopolymers
PubMed ID7055636
Fluorescence labeling of oligosaccharides useful in the determination of molecular interactions.
AuthorsSugahara D, Amano J, Irimura T
JournalAnal Sci
PubMed ID12558044
A simple method to label oligosaccharides with a multifunctional fluorescent group was developed. Oligosaccharides were quantitatively labeled at their reducing termini with pyrene butanoic acid hydrazide. The pyrene-labeled oligosaccharides were successfully applied to fluorescence polarization measurements and ELISA at picomole quantity, which was not previously reached by other procedures. This ... More
Nucleic acid hybridization accompanied with excimer formation from two pyrene-labeled probes.
AuthorsEbata K, Masuko M, Ohtani H, Kashiwasake-Jibu M
JournalPhotochem Photobiol
PubMed ID8570721
We developed a novel nucleic acid hybridization method based on excimer formation. We used two different 16-mer oligonucleotide probes that had a combined continuous-sequence run that was complementary to a target 32-mer. Prior to hybridization, the adjacent terminal ends (i.e. the 3'-terminal of one probe and the 5'-terminal of the ... More
New fluorescent hydrazide reagents for the oxidized 3'-terminus of RNA.
AuthorsReines SA, Cantor CR
JournalNucleic Acids Res
PubMed ID10793756
The synthesis and properties of four new fluorescent reagents capable of forming moderately stable links to the 3' oxidized end of RNA are reported. All are hydrazide derivatives: pyrene butyric acid hydrazide, proflavine monosemicarbazide, proflavine monosuccinic acid hydrazide, and anthracene-9-carboxaldehyde carbohydrazone. In addition, procedures are given for coupling the bifunctional ... More
Cross-linking and fluorescence of pyrene-labeled collagen.
AuthorsFujimori E, Shambaugh N
JournalBiochim Biophys Acta
PubMed ID6824679
The pyrene-labeling of acid-soluble (type I) and acid-insoluble collagens from young and old rat tail tendon has been investigated. The pyrene excimer fluorescence is associated with stabilized pyrene labels bound to two adjacent aldehydes in monomeric young collagens. Polymeric young collagens, as well as monomeric and polymeric old collagens, tend ... More
Fluorescent-labeled cross-links in collagen: pyrenesulfonylhydrazine.
AuthorsFujimori E, Shambaugh N
JournalBiochemistry
PubMed ID7295654
Aldol condensation products of two lysyl-derived aldehyde (allysine) residues are involved in cross-linking of collagen. However, the distribution of these cross-links and their age-related changes remain largely unanswered. We have found that the unsaturated aldehydes of aldol condensation cross-links can be fluorescent labeled. When labeled with pyrenesulfonylhydrazine, pyrene dimers and ... More