ProLong™ Gold 抗淬灭封片剂
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ProLong™ Gold 抗淬灭封片剂
ProLong™ Gold 抗淬灭封片剂
ProLong™ Gold 抗淬灭封片剂
Invitrogen™

ProLong™ Gold 抗淬灭封片剂

Green features
ProLong Gold 抗淬灭封片剂是一种直接用于显微玻片上荧光标记的细胞或组织样品的液态封片剂。其配方中不含 DNA 染色剂或含蓝色 DNA 染色剂 DAPI 或含深红色了解更多信息
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货号数量
P101442 mL
P369345 x 2 mL
P3693010 mL
货号 P10144
价格(CNY)
519.00
飞享价
Ends: 31-Dec-2025
698.00
共减 179.00 (26%)
Each
添加至购物车
数量:
2 mL
价格(CNY)
519.00
飞享价
Ends: 31-Dec-2025
698.00
共减 179.00 (26%)
Each
添加至购物车
ProLong Gold 抗淬灭封片剂是一种直接用于显微玻片上荧光标记的细胞或组织样品的液态封片剂。其配方中不含 DNA 染色剂或含蓝色 DNA 染色剂 DAPI 或含深红色 DNA 染色剂 SYTOX Deep Red。ProLong Gold 封片剂所含的化学组分可避免荧光染料在荧光显微镜实验中淬灭(光漂白),在初始荧光信号未明显淬灭的情况下提供这种保护。其是一种固化封片剂,可形成近乎完美的光程 (1.47 RI),确保样品可长期储存。其为即用型(室温储存且无需混合)—只需滴入样品、盖上玻片,随即可固化和成像。

不建议采用 ProLong Gold 抗淬灭封片剂封片含荧光蛋白(如 GFP)的样品。为对荧光蛋白和荧光染料提供出色的抗淬灭保护性能,建议使用 ProLong Diamond 抗淬灭封片剂。了解更多关于 ProLong Gold 抗淬灭封片剂与 ProLong Diamond 抗淬灭封片剂之间的主要差异的信息。

主要性质:

•在成像过程中避免染料褪色
• 即用型液体,封片后可长期储存
• 封固样品可保持稳定数月
• 维持荧光信号—几乎没有淬灭
• 适用于 Alexa Fluor 染料

折射率为 1.52 的封片剂,针对油浸物镜成像进行了优化,可尝试固化封片剂 Prolong Glass 抗淬灭封片剂或非固化 SlowFade Glass 软质抗淬灭封片剂

ProLong Gold 抗淬灭封片剂以预混形式提供,可直接在含/不含 DNA 染色剂的滴瓶或 10 mL 瓶内使用。DAPI 染色剂与 DNA 结合时由紫外光激发,激发波长为 360 nm,最大发射波长为 460 nm,使用 DAPI 传统滤光片检测。SYTOX Deep Red 染色剂与 DNA 结合时由红光激发,激发波长为 660 nm,最大发射波长为 682 nm,使用 Cy5/深红传统滤光片检测。

无需淬火即可获得光漂白保护
许多封片剂可以破坏初始荧光信号强度高达 80%。为打造 Molecular Probes Gold 系列封片剂,我们的科学家开发了一种化学添加剂配方,以最大限度避免广谱颜色和染料的初始信号淬灭。

成像及长期储存过程中信号稳定
我们的 FluoCells 系列使用基于环氧树脂的封片剂,制备的细胞可在数年内保持稳定,并提供最高水平的抗淬灭保护。但应用较不便,封固前需要在有机溶剂中操作数步。ProLong Gold 抗淬灭试剂是存档样品的绝佳替代选择。同时,这种试剂还是长时间成像过程中保持信号强度的绝佳选择,如在共聚焦激光扫描照明条件下重复扫描。其易于使用—可在空气中干燥,以除去过多的水分和封片剂。如果要立即观察样品,可选用我们的非固化封片剂SlowFade Gold 抗淬灭封片剂

不需丙烯酸树脂即可获得极佳的折射率匹配
对于现代高分辨率成像应用,折射率匹配至关重要。ProLong Gold 抗淬灭封片剂固化后折射率为1.47。此外,这种固化特性意味着您可避免使用脏污的丙烯酸树脂(即指甲油),如果丙烯酸树脂与现代显微镜物镜接触,可能会损坏或毁坏物镜。
仅供科研使用。不可用于诊断程序。
规格
描述ProLong™ Gold Antifade Mountant, 1 x 2 mL
环保功能危害更少、可持续包装
数量2 mL
运输条件室温
产品线ProLong™
产品类型抗淬灭固定剂
试剂类型抗荧光淬灭溶液
容积(公制)2 mL
Unit SizeEach
内容与储存
在室温下储存。

常见问题解答 (FAQ)

What is the difference between ProLong and SlowFade antifade reagents?

Our ProLong antifade reagents dispense as a liquid that will solidify upon the evaporation of water. SlowFade antifade reagents remain liquid. If you are going to image right away and then dispose of your sample, you do not need a mountant that hardens, such as the SlowFade reagents. If you wish to archive your slide for more than a day, you will want a mounting medium that hardens (or “cures”). This hardening will limit the off-rates of various dye-conjugated antibodies and provides a better refractive index. Also, there will be a lower diffusion rate of free radicals, limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

If I use ProLong Gold Antifade Mountant to mount my slides, should I seal the edges?

ProLong Gold Antifade Mountant hardens overnight at room temperature. For short-term storage (a couple of weeks) you do not need to seal the edges of the coverslip, and the sample should be stable. Beyond that time, though, some dye conjugates will have an off-rate into the medium, so cold storage is recommended. Sealing the edges will prevent long-term discoloration (golden color) from developing around the edges of the coverslip as the anti-oxidants oxidize.

The edges may be sealed with melted paraffin, VALAP (1:1:1 vaseline, lanolin, paraffin) or epoxy glue. Nail polish is not recommended as various components of nail polish may diffuse into the mountant and quench fluorescent dyes.

Find additional tips, troubleshooting help, and resources within our Cell Imaging Support Center.

Some antifade mounting media stay as liquid whereas others harden. What is the benefit of having one that hardens?

If you are going to image right away and then dispose of your sample, you probably want a mountant that does not harden. If you wish to archive your slide for more than a day, you want a mountant that hardens (or "cures"). This hardening will slow or prevent off-rate of your dye or conjugate and often produces a better refractive index. Secondary sealing is usually not necessary. Also there will be lower diffusion of free radicals, thus limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I mounted my cells in ProLong antifade mounting medium, but now I want to go back and re-label them. Is there a way I can unmount the coverslip after it has cured (hardened)?

Yes. Put the slide in a Coplin jar or beaker filled with warm (37oC) PBS buffer and let it sit, no agitation is required. The hardened ProLong mountant will swell and may slide off or be easily dislodged. If cells are adherent to the coverslip, make certain the coverslip side containing the cell or tissue sample does not land face down in the container or become scratched upon handling. Remove the coverslip, wash a couple of times, and proceed with re-staining and re-mounting in new ProLong mountant.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am using ProLong antifade mounting medium. Do I need to let it cure before imaging? Do I need to seal the edges of the coverslip?

You can image before it cures (hardens), and it will still slow photobleaching, but you have to let it cure overnight to get the best refractive index (resolution). There is no need to seal the edges. In fact, if you seal before it cures, it won't cure correctly. If you are archiving the slide for more than a month, though, seal the edges with resin, paraffin or VALAP (1:1:1 vaseline, lanolin, paraffin) after it cures or there may be slight discoloration along the edges over time.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.