4-aminopyridine decreases progesterone production by porcine granulosa cells.
AuthorsLi Y, Ganta S, von Stein FB, Mason DE, Mitchell BM, Freeman LC
JournalReprod Biol Endocrinol
PubMed ID12740033
'BACKGROUND: Ion channels occur as large families of related genes with cell-specific expression patterns. Granulosa cells have been shown to express voltage-gated potassium channels from more than one family. The purpose of this study was to determine the effects of 4-aminopyridine (4-AP), an antagonist of KCNA but not KCNQ channels. ... More
Protein kinase G activation of K(ATP) channels in human-cultured prostatic stromal cells.
AuthorsCook AL, Frydenberg M, Haynes JM
JournalCell Signal
PubMed ID12359308
'In this study, we identify and investigate the role of protein kinase G (PKG) in cells cultured from human prostatic stroma. Cells were used for immunocytochemistry, contractility or K(+) fluorescent imaging studies. All cultured prostatic stromal cells showed PKG immunostaining. Phorbol 12,13 diacetate (PDA, 1 microM) elicited contractions from human-cultured ... More
N-methyl-D-aspartate excitotoxicity: relationships among plasma membrane potential, Na(+)/Ca(2+) exchange, mitochondrial Ca(2+) overload, and cytoplasmic concentrations of Ca(2+), H(+), and K(+).
AuthorsKiedrowski L
JournalMol Pharmacol
PubMed ID10462550
'A high cytoplasmic Na(+) concentration may contribute to N-methyl-D-aspartate (NMDA)-induced excitotoxicity by promoting Ca(2+) influx via reverse operation of the Na(+)/Ca(2+) exchanger (NaCaX), but may simultaneously decrease the electrochemical Ca(2+) driving force by depolarizing the plasma membrane (PM). Digital fluorescence microscopy was used to compare the effects of Na(+) versus ... More
Determination of the physical environment within the Chlamydia trachomatis inclusion using ion-selective ratiometric probes.
AuthorsGrieshaber S, Swanson JA, Hackstadt T
JournalCell Microbiol
PubMed ID12027956
'Chlamydia trachomatis is an obligate intracellular bacterium with a biphasic life cycle that takes place entirely within a membrane-bound vacuole termed an inclusion. The chlamydial inclusion is non-fusogenic with endosomal or lysosomal compartments but intersects a pathway involved in transport of sphingomyelin from the Golgi apparatus to the plasma membrane. ... More
Dynamics and consequences of potassium shifts in skeletal muscle and heart during exercise.
AuthorsSejersted OM, Sjøgaard G
JournalPhysiol Rev
PubMed ID11015618
'Since it became clear that K(+) shifts with exercise are extensive and can cause more than a doubling of the extracellular [K(+)] ([K(+)](s)) as reviewed here, it has been suggested that these shifts may cause fatigue through the effect on muscle excitability and action potentials (AP). The cause of the ... More
Intracellular mediators of granulysin-induced cell death.
AuthorsOkada S, Li Q, Whitin JC, Clayberger C, Krensky AM
JournalJ Immunol
PubMed ID12928406
'Granulysin, a molecule present in the granules of CTL and NK cells, is cytolytic against microbes and tumors. Granulysin induces apoptosis of mammalian cells by damaging mitochondria and causing the release of cytochrome c and apoptosis-inducing factor, resulting in DNA fragmentation. Here we show that Ca2+ and K+ channels as ... More
Photoactivated azido fatty acid irreversibly inhibits anion and proton transport through the mitochondrial uncoupling protein.
AuthorsJezek P, Hanus J, Semrad C, Garlid KD
JournalJ Biol Chem
PubMed ID8626410
'The protonophoretic function of uncoupling protein (UCP) is activated by fatty acids. According to the "docking site" hypothesis (Jezek, P., and Garlid, K. D., J. Biol. Chem. 265, 19303-19311, 1990), the fatty acid binding site is identical with the anion channel of UCP. Skulachev (Skulachev, V. P. (1991) FEBS Lett. ... More
Uncoupling cell shrinkage from apoptosis reveals that Na+ influx is required for volume loss during programmed cell death.
AuthorsBortner CD, Cidlowski JA
JournalJ Biol Chem
PubMed ID12821680
'Cell shrinkage, or the loss of cell volume, is a ubiquitous characteristic of programmed cell death that is observed in all examples of apoptosis, independent of the death stimulus. This decrease in cell volume occurs in synchrony with other classical features of apoptosis. The molecular basis for cell shrinkage during ... More
A primary role for K+ and Na+ efflux in the activation of apoptosis.
AuthorsBortner CD, Hughes FM, Cidlowski JA
JournalJ Biol Chem
PubMed ID9405453
'Cell shrinkage is a major characteristic of apoptosis, but the mechanism and role of this process in cell death are poorly understood. The primary factor that controls volume regulation in all cells is ions, and thus we have examined the movement of ions at the single cell level in lymphocytes ... More
Mitochondrial K+ as modulator of Ca(2+)-dependent cytotoxicity in hepatocytes. Novel application of the K(+)-sensitive dye PBFI (K(+)-binding benzofuran isophthalate) to assess free mitochondrial K+ concentrations.
AuthorsZoeteweij JP, van de Water B, de Bont HJ, Nagelkerke JF
JournalBiochem J
PubMed ID8172616
'In isolated rat hepatocytes a sustained high increase in intracellular free Ca2+ ([Ca2+]i), induced by extracellular ATP, is associated with mitochondrial dysfunction and cell death. The Ca(2+)-induced effects are Pi-dependent and less severe when the intracellular K+ content is low. In this study, the involvement of mitochondrial K+ processing in ... More
Adrenoceptor-induced changes of intracellular K+ and Ca2+ in astrocytes and neurons in rat cortical primary cultures.
AuthorsMuyderman H, Hansson E, Nilsson M
JournalNeurosci Lett
PubMed ID9464648
'The calcium- and potassium sensitive fluorescent dyes fura-2 and K+-binding benzofuran isophtalate (PBFI) were used to detect changes in [Ca2+]i and [K+]i in type 1 astrocytes and neurons in mixed astroglial/neuronal rat cortical primary cultures after adrenoceptor stimulation. Noradrenalin (NA), phenylephrine (phe; alpha1-agonist), clonidine (clon; alpha2-agonist) and isoproterenol (iso; beta-agonist) ... More
Cationic gradient reversal and cytoskeleton-independent volume regulatory pathways define an early stage of apoptosis.
AuthorsBortner CD, Sifre MI, Cidlowski JA,
JournalJ Biol Chem
PubMed ID18187415
'Cell shrinkage, or apoptotic volume decrease (AVD), is a ubiquitous characteristic of programmed cell death that is independent of the death stimulus and occurs in all examples of apoptosis. Here we distinguished two specific stages of AVD based on cell size and a unique early reversal of intracellular ions that ... More
Intracellular potassium stabilizes human ether-à-go-go-related gene channels for export from endoplasmic reticulum.
AuthorsWang L, Dennis AT, Trieu P, Charron F, Ethier N, Hebert TE, Wan X, Ficker E,
JournalMol Pharmacol
PubMed ID19139152
'Several therapeutic compounds have been identified that prolong the QT interval on the electrocardiogram and cause torsade de pointes arrhythmias not by direct block of the cardiac potassium channel human ether-à-go-go-related gene (hERG) but via disruption of hERG trafficking to the cell surface membrane. One example of a clinically important ... More
Differential involvement of initiator caspases in apoptotic volume decrease and potassium efflux during Fas- and UV-induced cell death.
AuthorsVu CC, Bortner CD, Cidlowski JA
JournalJ Biol Chem
PubMed ID11431480
'Caspase activation and apoptotic volume decrease are fundamental features of programmed cell death; however, the relationship between these components is not well understood. Here we provide biochemical and genetic evidence for the differential involvement of initiator caspases in the apoptotic volume decrease during both intrinsic and extrinsic activation of apoptosis. ... More
Cardiac glycosides stimulate Ca2+ increases and apoptosis in androgen-independent, metastatic human prostate adenocarcinoma cells.
AuthorsMcConkey DJ, Lin Y, Nutt LK, Ozel HZ, Newman RA
JournalCancer Res
PubMed ID10919654
'Cardiac glycosides are used clinically to increase contractile force in patients with cardiac disorders. Their mechanism of action is well established and involves inhibition of the plasma membrane Na+/K+-ATPase, leading to alterations in intracellular K+ and Ca(2+) levels. Here, we report that the cardiac glycosides oleandrin, ouabain, and digoxin induce ... More
Caspase independent/dependent regulation of K(+), cell shrinkage, and mitochondrial membrane potential during lymphocyte apoptosis.
AuthorsBortner CD, Cidlowski JA
JournalJ Biol Chem
PubMed ID10419518
'The loss of cell volume is a fundamental feature of apoptosis. We have previously shown that DNA degradation and caspase activity occur only in cells which have shrunken as a result of potassium and sodium efflux (Bortner, C. D., Hughes, F. M., Jr., and Cidlowski, J. A. (1997) J. Biol. ... More
Tetramethylpyrazine as potassium channel opener to lower calcium influx into cultured aortic smooth muscle cells.
AuthorsTsai CC, Lai TY, Huang WC, Yang T, Liu IM, Wong KL, Chan P, Cheng JT
JournalPlanta Med
PubMed ID12865978
'In the present study, the effect of tetramethylpyrazine (TMP) on calcium (Ca 2+) influx was investigated in cultured vascular smooth muscle (A7r5) cells using Fura-2 as an indicator. The increase of Ca 2+ concentration in A7r5 cells produced by vasopressin or phenylephrine was attenuated by TMP from 0.01 micromol/L to ... More
Alkylsulfonates as probes of uncoupling protein transport mechanism. Ion pair transport demonstrates that direct H(+) translocation by UCP1 is not necessary for uncoupling.
AuthorsJaburek M, Varecha M, Jezek P, Garlid KD
JournalJ Biol Chem
PubMed ID11468281
'The mechanism of fatty acid-dependent uncoupling by mitochondrial uncoupling proteins (UCP) is still in debate. We have hypothesized that the anionic fatty acid head group is translocated by UCP, and the proton is transported electroneutrally in the bilayer by flip-flop of the protonated fatty acid. Alkylsulfonates are useful as probes ... More
The effect of mechanical deformation on the distribution of ions in fibroblasts.
AuthorsLeeves MA, McDonald F
JournalAm J Orthod Dentofacial Orthop
PubMed ID7771368
'The extracellular and intracellular sodium, potassium and chloride concentrations were determined in fibroblast cells located in the rat calvarium. The ionic values were determined by fluorescence microscopy after incubation with the fluorescent probes, sodium-binding benzofuran isophthalate (SBFI), potassium-binding benzofuran isophthalate (PBFI) and 6-methoxy-N-(3-sulfopropyl) quinolinium (SPQ) (Dyes were supplied by CALBIOCHEM, ... More
Fatty acid binding site of the mitochondrial uncoupling protein. Demonstration of its existence by EPR spectroscopy of 5-DOXYL-stearic acid.
AuthorsJezek P, Freisleben HJ
JournalFEBS Lett
PubMed ID8163011
Fatty acid binding site on isolated mitochondrial uncoupling protein (UcP) is demonstrated using EPR spectroscopy of 5-DOXYL-stearic acid (5-SASL), which also activated H+ transport in proteoliposomes containing UcP. In the presence of UcP the EPR spectrum showed reproducible broadening of the low field peak as well as an increase in ... More
Cytoprotective role of Ca2+- activated K+ channels in the cardiac inner mitochondrial membrane.
AuthorsXu W, Liu Y, Wang S, McDonald T, Van Eyk JE, Sidor A, O'Rourke B
JournalScience
PubMed ID12411707
Ion channels on the mitochondrial inner membrane influence cell function in specific ways that can be detrimental or beneficial to cell survival. At least one type of potassium (K+) channel, the mitochondrial adenosine triphosphate-sensitive K+ channel (mitoKATP), is an important effector of protection against necrotic and apoptotic cell injury after ... More
Bundle sheath cells of small veins in maize leaves are the location of uptake from the xylem.
AuthorsKeunecke M, Lindner B, Seydel U, Schulz A, Hansen UP
JournalJ Exp Bot
PubMed ID11413207
Rb(+) as a tracer for K(+) was used to test the hypothesis that uptake of K(+) from xylem vessels of small veins into the symplast of maize leaves occurs at the xylem/bundle sheath cell interface. 22.5 min after immersing cut leaves into 20 mM RbCl+1 mM KCl, Rb(+) appeared in ... More
Alteration of intracellular potassium and sodium concentrations correlates with induction of cytopathic effects by human immunodeficiency virus.
Increases in intracellular concentrations of potassium ([K+]i) and sodium ([Na+]i) occur concomitantly with cytopathic effects induced in a CD4+ T-lymphoblastoid cell line acutely infected by human immunodeficiency virus (HIV). This [K+]i increase was greater in cells infected by cytopathic HIV strains than in cells infected by less cytopathic strains. T ... More
Glutathione depletion and disruption of intracellular ionic homeostasis regulate lymphoid cell apoptosis.
Intracellular glutathione (GSH) depletion is an important hallmark of apoptosis. We have recently shown that GSH depletion by its extrusion regulates apoptosis independently of excessive reactive oxygen species accumulation. However, the mechanisms by which GSH depletion regulates apoptosis are still unclear. Because disruption of intracellular ionic homeostasis, associated with apoptotic ... More
Intracellular K+ suppresses the activation of apoptosis in lymphocytes.
AuthorsHughes FM, Bortner CD, Purdy GD, Cidlowski JA
JournalJ Biol Chem
PubMed ID9374553
Little is known about the mechanisms of suppression of apoptosis. We have addressed the novel possibility that the level of intracellular K+ regulates the apoptotic process by controlling the activity of death enzymes. We show that K+, at normal intracellular levels, inhibits both apoptotic DNA fragmentation and caspase-3(CPP32)-like protease activation, ... More
Selective role of intracellular chloride in the regulation of the intrinsic but not extrinsic pathway of apoptosis in Jurkat T-cells.
AuthorsHeimlich G, Cidlowski JA
JournalJ Biol Chem
PubMed ID16299378
Apoptosis is a genetic program for the removal of unwanted cells from an organism, which is distinct from necrosis by its characteristic volume loss or apoptotic volume decrease. This cell shrinkage is the result of ion redistribution that is crucial for both the activation and execution of apoptosis. Here we ... More
Intracellular ionic variations in the apoptotic death of L cells by inhibitors of cell cycle progression.
AuthorsBarbiero G, Duranti F, Bonelli G, Amenta JS, Baccino FM
JournalExp Cell Res
PubMed ID7698242
Treatment with VP-16 (1-50 microM) or excess thymidine (5 mM) caused a block of L cells at different steps in their progression through the replicative cycle. The arrest was followed by an asynchronous process of cell death that conformed to criteria for apoptosis. Careful monitoring of this process in the ... More
Identification and properties of a novel intracellular (mitochondrial) ATP-sensitive potassium channel in brain.
AuthorsBajgar R, Seetharaman S, Kowaltowski AJ, Garlid KD, Paucek P
JournalJ Biol Chem
PubMed ID11441006
Protection of heart against ischemia-reperfusion injury by ischemic preconditioning and K(ATP) channel openers is known to involve the mitochondrial ATP-sensitive K(+) channel (mitoK(ATP)). Brain is also protected by ischemic preconditioning and K(ATP) channel openers, and it has been suggested that mitoK(ATP) may also play a key role in brain protection. ... More
Human immunodeficiency virus infection of T-lymphoblastoid cells reduces intracellular pH.
Alterations in plasma membrane function are induced by many cytopathic viruses, including human immunodeficiency virus type 1 (HIV-1). These alterations can result in changes in the intracellular content of ions and other small molecules and can contribute to cytolysis and death of the infected cell. The pH-sensitive fluorescent probe 2',7'-bis(2-carboxyethyl)-5,6-carboxyfluorescein-acetoxymethyl ... More
Xenorhabdus nematophila (enterobacteriacea) secretes a cation-selective calcium-independent porin which causes vacuolation of the rough endoplasmic reticulum and cell lysis.
AuthorsRibeiro C, Vignes M, Brehélin M
JournalJ Biol Chem
PubMed ID12441337
Xenorhabdus nematophila and Photorhabdus luminescens are two related enterobacteriaceae studied for their use in biological control and for synthesis of original virulence factors and new kinds of antibiotics. X. nematophila broth growth exhibits different cytotoxic activities on insect (Spodoptera littoralis, lepidoptera) immunocytes (hemocytes). Here we report the purification of the ... More
Differential ion accumulation and ion fluxes in the mesophyll and epidermis of barley.
AuthorsKarley AJ, Leigh RA, Sanders D
JournalPlant Physiol
PubMed ID10712547
In barley (Hordeum vulgare L.) leaves, differential ion accumulation commonly results in inorganic phosphate (Pi) being confined to the mesophyll and Ca(2+) to the epidermis, with preferential epidermal accumulation of Cl(-), Na(+), and some other ions. The pattern was confirmed in this study for major inorganic anions and cations by ... More
Passive nitrate transport by root plasma membrane vesicles exhibits an acidic optimal pH like the H(+)-ATPase.
AuthorsPouliquin P, Boyer JC, Grouzis JP, Gibrat R
JournalPlant Physiol
PubMed ID10631270
The net initial passive flux (J(Ni)) in reconstituted plasma membrane (PM) vesicles from maize (Zea mays) root cells was measured as recently described (P. Pouliquin, J.-P. Grouzis, R. Gibrat ¿1999 Biophys J 76: 360-373). J(Ni) in control liposomes responded to membrane potential or to NO(3)(-) as expected from the Goldman-Hodgkin-Katz ... More
Monocytic cell necrosis is mediated by potassium depletion and caspase-like proteases.
AuthorsWarny M, Kelly CP
JournalAm J Physiol
PubMed ID10070000
Apoptosis is a physiological cell death that culminates in mitochondrial permeability transition and the activation of caspases, a family of cysteine proteases. Necrosis, in contrast, is a pathological cell death characterized by swelling of the cytoplasm and mitochondria and rapid plasma membrane disruption. Necrotic cell death has long been opposed ... More
On the mechanism of fatty acid-induced proton transport by mitochondrial uncoupling protein.
AuthorsGarlid KD, Orosz DE, Modrianský M, Vassanelli S, Jezek P
JournalJ Biol Chem
PubMed ID8576230
Uncoupling protein mediates electrophoretic transport of protons and anions across the inner membrane of brown adipose tissue mitochondria. The mechanism and site of proton transport, the mechanism by which fatty acids activate proton transport, and the relationship between fatty acids and anion transport are unknown. We used fluorescent probes to ... More
Functional distinctions between the mitochondrial ATP-dependent K+ channel (mitoKATP) and its inward rectifier subunit (mitoKIR).
The ATP-sensitive potassium channel from the inner mitochondrial membrane (mitoK(ATP)) is a highly selective conductor of K(+) ions. When isolated in the presence of nonionic detergent and reconstituted in liposomes, mitoK(ATP) is inhibited with high affinity by ATP (K((1/2)) = 20-30 microM). We have suggested that holo-mitoK(ATP) is a heteromultimer ... More
More than 99% of ovarian follicles are lost by a degenerative process known as atresia, a phenomenon characterized by apoptosis of granulosa cells. Uniquely, dying granulosa cells also greatly increase their progesterone biosynthesis while reducing estrogen production. Recent studies have documented a dramatic decrease in intracellular K+ concentration during apoptosis ... More
Regulation of intracellular potassium in mesangial cells: a fluorescence analysis using the dye, PBFI.
AuthorsKasner SE, Ganz MB
JournalAm J Physiol
PubMed ID1558163
We investigated the regulatory transport processes that maintain intracellular K+ homeostasis in cultured rat glomerular mesangial cells (MCs). Intracellular K+ concentration ([K+]i) of quiescent MCs, passages 3-8, grown to subconfluence on glass cover slips, was assessed by spectrofluorometry using the K(+)-sensitive dye, K(+)-binding benzofuran isophthalate (PBFI). Serum-starved MCs were incubated ... More
Attenuation of apoptosis in enterocytes by blockade of potassium channels.
AuthorsGrishin A, Ford H, Wang J, Li H, Salvador-Recatala V, Levitan ES, Zaks-Makhina E
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID16020659
Apoptosis plays an important role in maintaining the balance between proliferation and cell loss in the intestinal epithelium. Apoptosis rates may increase in intestinal pathologies such as inflammatory bowel disease and necrotizing enterocolitis, suggesting pharmacological prevention of apoptosis as a therapy for these conditions. Here, we explore the feasibility of ... More
Increased K+ efflux and apoptosis induced by the potassium channel modulatory protein KChAP/PIAS3beta in prostate cancer cells.
AuthorsWible BA, Wang L, Kuryshev YA, Basu A, Haldar S, Brown AM
JournalJ Biol Chem
PubMed ID11877452
K(+) channel-associated protein/protein inhibitor of activated STAT (KChAP/PIAS3beta) is a potassium (K(+)) channel modulatory protein that boosts protein expression of a subset of K(+) channels and increases currents without affecting gating. Since increased K(+) efflux is an early event in apoptosis, we speculated that KChAP might induce apoptosis through its ... More
Effects of salinity on cytosolic Na+ and K+ in root hairs of Arabidopsis thaliana: in vivo measurements using the fluorescent dyes SBFI and PBFI.
AuthorsHalperin SJ, Lynch JP
JournalJ Exp Bot
PubMed ID12925666
Toxicity from sodium accumulation is an important aspect of salinity stress that has been well studied at the organ and tissue level. However, the effects of salinity on sodium accumulation in the cytosol, where much of the sodium toxicity is thought to occur, are poorly understood due to the difficulty ... More
Protein kinase C (PKC) inhibits fas receptor-induced apoptosis through modulation of the loss of K+ and cell shrinkage. A role for PKC upstream of caspases.
AuthorsGómez-Angelats M, Bortner CD, Cidlowski JA
JournalJ Biol Chem
PubMed ID10867019
Cell shrinkage and loss of intracellular K(+) are early requisite features for the activation of effector caspases and apoptotic nucleases in Fas receptor-mediated apoptosis of Jurkat cells, although the mechanisms responsible for both process remain unclear (Bortner, C. D., Hughes, F. M., Jr., and Cidlowski, J. A. (1997) J. Biol. ... More
Hemolysis of erythrocytes by granulysin-derived peptides but not by granulysin.
AuthorsLi Q, Dong C, Deng A, Katsumata M, Nakadai A, Kawada T, Okada S, Clayberger C, Krensky AM
JournalAntimicrob Agents Chemother
PubMed ID15616319
Granulysin, a 9-kDa protein localized in human cytolytic T lymphoctyes and natural killer cell granules, is cytolytic against tumors and microbes but not against red blood cells. Synthetic peptides corresponding to the central region of granulysin recapitulate the lytic activity of the intact molecule, and some peptides cause hemolysis of ... More
Aerolysin induces G-protein activation and Ca2+ release from intracellular stores in human granulocytes.
AuthorsKrause KH, Fivaz M, Monod A, van der Goot FG
JournalJ Biol Chem
PubMed ID9660770
Aerolysin is a pore-forming toxin that plays a key role in the pathogenesis of Aeromonas hydrophila infections. In this study, we have analyzed the effect of aerolysin on human granulocytes (HL-60 cells). Proaerolysin could bind to these cells, was processed into active aerolysin, and led to membrane depolarization, indicating that ... More
Pharmacologic characterization of BMS-191095, a mitochondrial K(ATP) opener with no peripheral vasodilator or cardiac action potential shortening activity.
Previous work described ATP-sensitive K(+) channel (K(ATP)) openers (e.g., BMS-180448), which retain the cardioprotective activity of agents such as cromakalim while being significantly less potent as vasodilators. In this study, we describe the pharmacologic profile of BMS-191095, which is devoid of peripheral vasodilating activity while retaining glyburide-reversible cardioprotective activity. In ... More
Burn trauma alters calcium transporter protein expression in the heart.
We have shown previously that burn trauma produces significant cardiac dysfunction, which is first evident 8 h postburn and is maximal 24 h postburn. Because calcium handling by the cardiomyocyte is essential for cardiac function, one mechanism by which burn injury may cause cardiac abnormalities is via calcium dyshomeostasis. We ... More
Store-operated Ca(2+) channels in human glomerular mesangial cells.
AuthorsMa R, Smith S, Child A, Carmines PK, Sansom SC
JournalAm J Physiol Renal Physiol
PubMed ID10836983
Experiments were performed to identify the biophysical properties of store-operated Ca(2+) channels (SOC) in cultured human glomerular mesangial cells (MC). A fluorometric technique (fura 2) was utilized to monitor the change in intracellular calcium concentration ([Ca(2+)](i)) evoked by elevating external [Ca(2+)] from 10 nM to 1 mM (Delta[Ca(2+)]). Under control ... More
Increase in cytosolic Ca2+ levels through the activation of non-selective cation channels induced by oxidative stress causes mitochondrial depolarization leading to apoptosis-like death in Leishmania donovani promastigotes.
AuthorsMukherjee SB, Das M, Sudhandiran G, Shaha C
JournalJ Biol Chem
PubMed ID11983701
Reactive oxygen species are important regulators of protozoal infection. Promastigotes of Leishmania donovani, the causative agent of Kala-azar, undergo an apoptosis-like death upon exposure to H2O2. The present study shows that upon activation of death response by H2O2, a dose- and time-dependent loss of mitochondrial membrane potential occurs. This loss ... More
Involvement of mitochondrial K+ release and cellular efflux in ischemic and apoptotic neuronal death.
AuthorsLiu D, Slevin JR, Lu C, Chan SL, Hansson M, Elmér E, Mattson MP
JournalJ Neurochem
PubMed ID12887694
We measured and manipulated intracellular potassium (K+) fluxes in cultured hippocampal neurons in an effort to understand the involvement of K+ in neuronal death under conditions of ischemia and exposure to apoptotic stimuli. Measurements of the intracellular K+ concentration using the fluorescent probe 1,3-benzenedicarboxylic acid, 4,4'-[1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diylbis(5-methoxy-6,2-benzofurandiyl)]bis-, tetrakis [(acetyloxy) methyl] ester ... More
Identification of potassium-dependent and -independent components of the apoptotic machinery in mouse ovarian germ cells and granulosa cells.
Recent studies with thymocytes have suggested a critical role for intracellular potassium in the regulation of apoptosis. In this study, we examined the pathways of K(+) regulation during ovarian cell death. In initial studies, fluorographic analysis demonstrated a significant loss of K(+) during apoptosis stimulated by doxorubicin in oocytes and ... More
Choline Uptake and Metabolism Modulate Macrophage IL-1β and IL-18 Production.
Authors
JournalCell Metab
PubMed ID30982734
Farnesoid X Receptor Regulation of the NLRP3 Inflammasome Underlies Cholestasis-Associated Sepsis.
Authors
JournalCell Metab
PubMed ID28380377
Changes in transepithelial electrical resistance and intracellular ion concentration in TGF-β-induced epithelial-mesenchymal transition of retinal pigment epithelial cells.