A microplate reader-based nonisotopic histone deacetylase activity assay.
AuthorsHeltweg B, Jung M
JournalAnal Biochem
PubMed ID11878795
'Recent years have brought an enormous increase in knowledge concerning the involvement of histone deacetylase (HDAC) in gene regulation and the potential use of its inhibitors in transcription therapy. This also stimulates research toward new methods for the determination of HDAC activity and thus the potency of potential inhibitors. We ... More
5-(Pentafluorobenzoylamino)fluorescein: A selective substrate for the determination of glutathione concentration and glutathione S-transferase activity.
AuthorsArttamangkul S, Bhalgat MK, Haugland RP, Diwu Z, Liu J, Klaubert DH, Haugland RP
JournalAnal Biochem
PubMed ID10222018
'5-(Pentafluorobenzoylamino)fluorescein (PFB-F), a new thiol-reactive molecule was synthesized to improve the detection limits and specificity of the assays for glutathione S-transferase (GST) activity and glutathione (GSH). A rapid assay method to measure GSH concentration or GST activity and the simultaneous analysis of multiple samples is possible because the glutathione adduct, ... More
Analytical methods to investigate glutathione and related compounds in biological and pathological processes.
AuthorsCamera E, Picardo M
JournalJ Chromatogr B Analyt Technol Biomed Life Sci
PubMed ID12450659
Reduced glutathione (GSH, gamma-L-glutamyl-L-cysteinylglycine) is a fundamental low-molecular mass antioxidant that serves several biological functions. Upon enzymatic and non-enzymatic oxidation, GSH forms glutathione disulfide (GSSG) and, under particular conditions, may generate other oxidative products. The determination of GSH, its precursors, and metabolites in several bio-matrices is a useful tool in ... More