碘化丙啶

碘化丙啶 (PI) 是一种常用的红色荧光细胞核和染色体复染剂。由于碘化丙啶不能渗透到活细胞中，也通常用于检测群体中的死细胞。PI 通过在碱基之间进行插入而与 DNA 结合，几乎没有序列偏好。在水溶液中，该染料的最大激发/发射波长为 493/636了解更多信息

货号	数量
P1304MP	100 mg

货号 P1304MP

价格（CNY)

1,967.00

Ends: 31-Dec-2025

2,612.00

共减 645.00 (25%)

添加至购物车

100 mg

价格（CNY)

1,967.00

Ends: 31-Dec-2025

2,612.00

共减 645.00 (25%)

添加至购物车

碘化丙啶 (PI) 是一种常用的红色荧光细胞核和染色体复染剂。由于碘化丙啶不能渗透到活细胞中，也通常用于检测群体中的死细胞。

PI 通过在碱基之间进行插入而与 DNA 结合，几乎没有序列偏好。在水溶液中，该染料的最大激发/发射波长为 493/636 nm。一旦染料被结合，其荧光增强 20 至 30 倍，荧光最大激发波长向红色偏移 ∼30–40 nm，荧光最大发射波长向蓝色偏移 ∼15 nm，使得最大激发波长为 535 nm，荧光最大发射波长为 617 nm。

PI 广泛用于荧光显微镜检查、共聚焦激光扫描显微镜检查、流式细胞分析和荧光测定法。

了解有关碘化丙啶和含碘化丙啶产品的更多信息

仅供科研使用。不可用于诊断程序。

建议的储存条件含1小瓶碘化丙啶 (100 mg)。室温避光储存。

物理形态Solid

数量100 mg

Unit SizeEach

常见问题解答 (FAQ)

I want to use propidium iodide and SYTO 9 to do LIVE/DEAD testing of a bacterial sample. Will anaerobic conditions adversely affect the assay?

Oxygen content should not affect the binding of propidium iodide and SYTO 9 to nucleic acids. SYTO 9 will label all cells, and propidium iodide will label only dead cells or cells with a compromised membrane.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have a LIVE/DEAD BacLight Bacterial Viability kit that has SYTO 9 and propidium iodide in it. Will I be able to stain eukaryotic cells that have engulfed bacteria and determine if the bacteria are alive or dead using this kit?

Unfortunately, no. SYTO 9 will label the nuclei of live or dead cells, including the eukaryotic cells. Propidium iodide is cell impermeant, and will only enter dead cells. If the eukaryotic cells are dead, they will label with propidium iodide as well. If the eukaryotic cells are alive, propidium iodide will not be able to enter and thus will not label the bacteria inside, whether the bacteria are alive or dead. We are not aware of any way to do a viability assay of bacteria once they have been engulfed by cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is propidium iodide (PI) fixable with glutaraldehyde or paraformaldehyde (PFA)?

PI is not fixable with glutaraldehyde or PFA. Both reagents fix by crosslinking amines. PI and other nucleic acid stains do not inherently bind covalently to nucleic acids and these fixatives do not crosslink the dyes to nucleic acids.
The one fixable nucleic acid stain is Ethidium Monoazide Bromide (EMA), Cat no. E1374); it covalently binds to nucleic acids upon activation by exposure to light.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.