Omega oxidation of 3-hydroxy fatty acids by the human CYP4F gene subfamily enzyme CYP4F11.
AuthorsDhar M, Sepkovic DW, Hirani V, Magnusson RP, Lasker JM,
JournalJ Lipid Res
PubMed ID18065749
'Long-chain 3-hydroxydicarboxylic acids (3-OHDCAs) are thought to arise via beta-oxidation of the corresponding dicarboxylic acids (DCAs), although long-chain DCAs are neither readily transported into nor beta-oxidized in mitochondria. We thus examined whether omega-hydroxylation of 3-hydroxy fatty acids (3-OHFAs), formed via incomplete mitochondrial oxidation, is a more likely pathway for 3-OHDCA ... More
Headspace solid-phase microextraction with 1-pyrenyldiazomethane in-fibre derivatisation for analysis of faecal short-chain fatty acids.
AuthorsMills GA, Walker V, Mughal H
JournalJ Chromatogr B Biomed Sci Appl
PubMed ID10437678
'Short chain fatty acids (SFCAs) are nutritionally important products of colonic bacteria. Their analysis in faeces is problematic. We report a headspace solid-phase microextraction procedure in which faecal SCFAs are derivatised on the fibre in-situ with 1-pyrenyldiazomethane. With this method sharp, well-resolved chromatographic peaks were obtained with no interferences. Inclusion ... More
[Highly effective separation and highly sensitive detection for clinical chemistry and biochemistry]
AuthorsKinoshita T
JournalYakugaku Zasshi
PubMed ID9513578
'Highly effective separation and highly sensitive detection reagents for clinical chemistry and biochemistry were developed and their applications were investigated. The sensitive detection of carboxylic acids was accomplished using 9-anthryldiazomethane (ADAM) which gave intensely fluorescent derivatives from carboxylic acids without catalysts or heating. 1-Pyrenyldiazomethane was then synthesized and proved to ... More
Application of capillary electrophoresis with laser-induced fluorescence detection for routine determination of methylmalonic acid in human serum.
AuthorsSchneede J, Ueland PM
JournalAnal Chem
PubMed ID7762817
'Methylmalonic acid (MMA) in serum is an established marker of cobalamin deficiency. MMA and other short-chain dicarboxylic acids react with 1-pyrenyldiazomethane to form stable, highly fluorescent 1-pyrenylmethyl monoesters. We have analyzed these esters in human blood by capillary electrophoresis (CE) combined with laser-induced fluorescence detection, and here we describe our ... More
Enhancement of hydrolytic activity of sphingolipid ceramide N-deacylase in the aqueous-organic biphasic system.
AuthorsKurita T, Izu H, Sano M, Ito M, Kato I
JournalJ Lipid Res
PubMed ID10787446
'Lysoglycosphingolipids were produced from glycosphingolipids by using sphingolipid ceramide N-deacylase, which cleaves the N-acyl linkage between fatty acids and sphingosine bases in various glycosphingolipids. The enzyme reaction was done in a biphasic media prepared with water;-immiscible organic solvent and aqueous buffer solution containing the enzyme. We investigated the effects of ... More
Nuclear localization of 5-lipoxygenase as a determinant of leukotriene B4 synthetic capacity.
AuthorsLuo M, Jones SM, Peters-Golden M, Brock TG
JournalProc Natl Acad Sci U S A
PubMed ID14530386
'The enzyme 5-lipoxygenase (5-LO) initiates the synthesis of leukotrienes from arachidonic acid. In resting cells, 5-LO can accumulate in either the cytoplasm or the nucleoplasm and, upon cell stimulation, translocates to membranes to initiate leukotriene synthesis. Here, we used mutants of 5-LO with altered subcellular localization to assess the role ... More
Automated assay of methylmalonic acid in serum and urine by derivatization with 1-pyrenyldiazomethane, liquid chromatography, and fluorescence detection.
AuthorsSchneede J, Ueland PM
JournalClin Chem
PubMed ID8448848
'Determination of methylmalonic acid (MMA) in serum has been established as an accurate test for the diagnosis of cobalamin deficiency. We describe here the development and performance of a liquid-chromatographic assay of MMA in blood and urine. The assay is based on our recent finding that one of the carboxylic ... More
Evaluation of proposed sulphoxidation pathways of carbocysteine in man by HPLC quantification.
AuthorsBrockmöller J, Staffeldt B, Roots I
JournalEur J Clin Pharmacol
PubMed ID2050173
'A quantitative study has been made of the metabolism of S-carboxymethyl-L-cysteine (CMC) and its sulphoxides in volunteers by HPLC. Precolumn derivatization was applied prior to gradient reversed phase HPLC separation and fluorescence detection. For CMC and its metabolites containing a primary amino group the reagent 9-fluorenylmethylchloroformate was used. The other ... More
Analysis of cardiolipin in human muscle biopsy.
AuthorsRitov VB, Menshikova EV, Kelley DE
JournalJ Chromatogr B Analyt Technol Biomed Life Sci
PubMed ID16337440
'Cardiolipin is a phospholipid that is specific to the inner mitochondrial membrane and essential for numerous mitochondrial functions. Accordingly, a quantitative assay for cardiolipin can be a valuable aspect of assessing mitochondrial content and functional capacity. The current study was undertaken to develop a simple and reliable method for direct ... More
1-Pyrenyldiazomethane as a fluorescent labeling reagent for liquid chromatographic determination of carboxylic acids.
AuthorsNimura N, Kinoshita T, Yoshida T, Uetake A, Nakai C
JournalAnal Chem
PubMed ID3239787
Liquid chromatographic determination of biotin by using 1-pyrenyldiazomethane as a pre-column fluorescent labelling reagent.
AuthorsYoshida T, Uetake A, Nakai C
JournalJ Chromatogr
PubMed ID3243878
Pyrenyldiazomethane, a versatile reagent for nucleotide phosphate alkylation.
AuthorsKotera M, Dheu ML, Milet A, Lhomme J, Laayoun A,
JournalBioorg Med Chem Lett
PubMed ID15664841
Pyrenyldiazomethane was shown to react quantitatively and selectively at phosphate with 2'-, 3'-, and 5'-nucleotide phosphates incorporating the different nucleic bases.
Labeling of free carboxyl groups.
AuthorsYasaka Y, Tanaka M
JournalJ Chromatogr B Biomed Appl
PubMed ID7820274
The latest trends in the labeling of free carboxyl groups for high-performance liquid chromatography are reviewed. The labeling reagents for fluorescence detection are mainly discussed according to their reaction type (or functional group). Attention is also paid to the reagents used for ultraviolet detection and for enantiomeric separation. The reactivity ... More
Ultraviolet and fluorescence derivatization reagents for carboxylic acids suitable for high performance liquid chromatography: a review.
AuthorsMukherjee PS, Karnes HT
JournalBiomed Chromatogr
PubMed ID8879524
Pre- and postcolumn derivatization with a suitable chromophore or fluorophore have often been utilized to enhance the sensitivity and selectivity of detection for analytes possessing a carboxyl group. The major classes of UV and fluorescent derivatization reagents include the coumarin analogues, alkyl halides, diazoalkanes, and amines. The derivatization reaction conditions, ... More
Derivatization reactions for neurotransmitters and their automation.
AuthorsBovingdon ME, Webster RA
JournalJ Chromatogr B Biomed Appl
PubMed ID7820275
Many reagents suitable for the derivatization of neurotransmitters are selective for the amino function. Others, however, are selective for the carboxyl-, thiol- and hydroxyl function, and recently, reagents selective for more than one function have been produced. Interest persists in the established reagents, with their well understood behaviour which assists ... More
Determination of lipid profile in meningococcal polysaccharide using reversed-phase liquid chromatography.
AuthorsLi Y, Lander R, Manger W, Lee A
JournalJ Chromatogr B Analyt Technol Biomed Life Sci
PubMed ID15081930
A fast and sensitive HPLC method using fluorescence detection is developed to quantitate 1-pyrenyldiazomethane (PDAM) derivatized fatty acids derived from the lipid components of both the capsular meningococcal polysaccharide and other impurities such as endotoxin in various meningococcal vaccine samples. The HPLC method is capable of well resolving 13 relevant ... More
High-performance liquid chromatographic determination of cortolic and cortolonic acids as pyrenyl ester derivatives.
AuthorsIohan F, Vincze I, Monder C, Cohen S
JournalJ Chromatogr
PubMed ID1860920
A new procedure is described for the detection of the acidic metabolites of cortisol (cortoic acids) as the pyrenylmethyl-21-oic esters. The derivatizing reagent, diazomethylpyrene, was prepared by an improved procedure. The reagent was used at room temperature, required no catalyst, and was not restricted by stoichiometric requirements. The steroid esters ... More
Fluorescent HPLC assay for 20-HETE and other P-450 metabolites of arachidonic acid.
AuthorsMaier KG, Henderson L, Narayanan J, Alonso-Galicia M, Falck JR, Roman RJ
JournalAm J Physiol Heart Circ Physiol
PubMed ID10924088
This study describes a fluorescent HPLC assay for measuring 20-hydroxyeicosatetraenoic acid (20-HETE) and other cytochrome P-450 metabolites of arachidonic acid in urine, tissue, and interstitial fluid. An internal standard, 20-hydroxyeicosa-6(Z),15(Z)-dienoic acid, was added to samples, and the lipids were extracted and labeled with 2-(2,3-naphthalimino)ethyl trifluoromethanesulfonate. P-450 metabolites were separated on ... More
Tumor necrosis factor-induced release of endogenous fatty acids analyzed by a highly sensitive high-performance liquid chromatography method.
AuthorsBrekke OL, Sagen E, Bjerve KS
JournalJ Lipid Res
PubMed ID9323601
A highly sensitive method to determine agonist-induced release of endogenous fatty acids from cells in culture was developed using high-performance liquid chromatography and fluorescence detection. Fatty acids were selectively derivatized with 1-pyrenyldiazomethane and separated on a LC18 reversed phase column using an acetonitrile-water gradient. The detection limit was approx. 20 ... More
Liquid chromatographic methods for the isolation and identification of new pectenotoxin-2 analogues from marine phytoplankton and shellfish.
AuthorsJames KJ, Bishop AG, Draisci R, Palleschi L, Marchiafava C, Ferretti E, Satake M, Yasumoto T
JournalJ Chromatogr A
PubMed ID10399323
Two acidic analogues of the polyether marine toxin, pectenotoxin-2 (PTX-2), responsible for diarrhetic shellfish poisoning (DSP), have been isolated from the toxic marine phytoplankton (Dinophysis acuta), collected in Irish waters. Liquid chromatography with fluorimetric detection (LC-FLD) analyses of the extracts of bulk phytoplankton samples, following derivatisation with 9-anthryldiazomethane (ADAM) or ... More
Specificity of endogenous fatty acid release during tumor necrosis factor-induced apoptosis in WEHI 164 fibrosarcoma cells.
AuthorsBrekke OL, Sagen E, Bjerve KS
JournalJ Lipid Res
PubMed ID10588948
Recombinant tumor necrosis factor alpha (rTNF-alpha)-induced release of endogenous fatty acids was examined in WEHI 164 clone 13 fibrosarcoma cells using a highly sensitive HPLC method. The initial rTNF-alpha-induced extracellular release of endogenous fatty acids was dominated by 20:4n;-6, 22:4n;-6, 24:4n;-6, and 18:1n;-9 showing relative rates of 2.9, 0.9, 1.1, ... More
Oxidative stress following traumatic brain injury in rats: quantitation of biomarkers and detection of free radical intermediates.
AuthorsTyurin VA, Tyurina YY, Borisenko GG, Sokolova TV, Ritov VB, Quinn PJ, Rose M, Kochanek P, Graham SH, Kagan VE
JournalJ Neurochem
PubMed ID11032908
Oxidative stress may contribute to many pathophysiologic changes that occur after traumatic brain injury. In the current study, contemporary methods of detecting oxidative stress were used in a rodent model of traumatic brain injury. The level of the stable product derived from peroxidation of arachidonyl residues in phospholipids, 8-epi-prostaglandin F(2alpha), ... More
Quantitation of diarrhetic shellfish poisoning toxins in Chilean mussel using pyrenyldiazomethane as fluorescent labeling reagent.
AuthorsGarcía C, Pereira P, Valle L, Lagos N
JournalBiol Res
PubMed ID14513712
Diarrhetic shellfish poisoning (DSP) is a gastrointestinal disease caused by lipid soluble polyether toxins produced by dinoflagellates and accumulated in shellfish. Diarrhetic shellfish poisoning is a worldwide threat to public health and the shellfish industry. To date, only four lipid soluble polyethers have been known as diarrhetic shellfish toxins. Among ... More