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Citations & References (17)
Invitrogen™
PiPer™ Pyrophosphate or Phosphate Assay Kit
PiPer Pyrophosphate Assay Kit provides reagents for fluorogenic or colorimetric detection as low as 0.4 mμM (fluorescence) or 2 μM (absorbance) inorganic pyrophosphate (PPi) while Phosphate Assay kits can detect down to 0.2 μM Pi in purified enzyme systems. Each kit has enough for 1000 labelings.
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| Catalog Number | Product Type |
|---|---|
| P22061 | Phosphate Assay |
| P22062 | Pyrophosphate Assay |
Catalog number P22061
Price (CNY)
7,235.00
Each
Product Type:
Phosphate Assay
Price (CNY)
7,235.00
Each
PiPer Pyrophosphate and Phosphate Assay Kit provides an ultrasensitive assay to detect the fluorescent product resorufin. These kits can be used to detect inorganic pyrophosphate (PPi) or phosphate (Pi) in a variety of samples or can monitor the kinetics of their release by a variety of enzymes, including DNA/RNA polymerases, adenylate cyclase, S-acetyl coenzyme A synthetase, ATPases, GTPases, 5′-nucleotidase, protein phosphatases, acid/alkaline phosphatases and phosphorylase kinase. As resorufin has a strong absorption, this assay can be performed either fluorometrically or colorimetrically.
Key Features:
- High Sensitivity: Can detect low levels of inorganic phosphate
- Number of labelings per kit: ~1,000 of 100 μL per assay volume
- Detection method: Colorimetric, Fluorescence
- Compatibility: Can be used with both microplate readers and spectrophotometers
- Excitation/emission maxima: 563/587 nm
- Versatility: Suitable for a wide range of applications including enzyme activity assays
- Quantitative Results: Provides accurate and reproducible results.
The PiPer Pyrophosphate Assay Kit provides a sensitive fluorogenic or colorimetric method for detecting as little as 0.4 μM (fluorescence) or 2 μM (absorbance) inorganic pyrophosphate in a 100 μL assay volume. The PiPer Phosphate Assay Kit detects as little as 0.2 μM Pi (fluorescence) in purified enzyme systems with a 100 μL assay volume.
Use the PiPer Pyrophosphate and Phosphate Assay Kit for applications such as: Anion Detection, Biochemical Toxicology Assays, Cell Analysis, Cell Metabolism, Cell Viability, Cellular Toxicology Assays, Drug Discovery and Development, Enzyme and Protein Activity Assays, Ionic Homeostasis and Signaling, Phosphatase Activity, Phosphatase Activity Assays, Phosphatase Biology, Target and Lead Identification and Validation.
The absorption and fluorescence of resorufin are pH dependent. There is a pKa at ~6.0 where the absorption maximum shifts to ~480 nm and the fluorescence quantum yield is markedly lower. Given Amplex™ Red reagent is unstable at high pH (>8.5) and is sensitive to light, we recommend this reaction be performed using the provided reaction buffer (at optimal pH ~7.5) for best results and reagents kept protected from light. All kits should follow user manual guidelines.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodColorimetric, Fluorescence
Dye TypeAmplex™ Red
Excitation/Emission563/587 nm
Format96-well plate
Quantity1000 Assays
Shipping ConditionRoom Temperature
ColorRed
For Use With (Application)Phosphate Assay
For Use With (Equipment)Spectrophotometer, Microplate Reader
Product LinePiPer
Product TypePhosphate Assay
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.
Frequently asked questions (FAQs)
How does the PiPer Phosphate Assay kit work?
Citations & References (17)
Citations & References
Abstract
Enzymatic analysis of lipid phosphate phosphatases.
Journal:Methods
PubMed ID:16815033
'Lipid phosphate monoesters including phosphatidic acid, lysophosphatidic acid, sphingosine 1-phosphate and ceramide 1-phosphate are intermediates in phosho- and sphingo-lipid biosynthesis and also play important roles in intra- and extra-cellular signaling. Dephosphorylation of these lipids terminates their signaling actions and, in some cases, generates products with additional biological activities or metabolic
Discovery of acetyl-coenzyme A carboxylase 2 inhibitors: comparison of a fluorescence intensity-based phosphate assay and a fluorescence polarization-based ADP Assay for high-throughput screening.
Journal:Assay Drug Dev Technol
PubMed ID:17477831
'Acetyl-coenzyme A carboxylase (ACC) enzymes exist as two isoforms, ACC1 and ACC2, which play critical roles in fatty acid biosynthesis and oxidation. Though each isoform differs in tissue and subcellular localization, both catalyze the biotin- and ATP-dependent carboxylation of acetyl-coenzyme A to generate malonyl-coenzyme A, a key metabolite in the
High-throughput screening for Hsp90 ATPase inhibitors.
Journal:Bioorg Med Chem Lett
PubMed ID:16530412
Recently, we reported a useful assay for the determination of yeast Hsp90 ATPase activity. Using this assay, high-throughput screening of approximately 10,000 compounds was performed to determine the feasibility of this assay on large scale. Results from high-throughput screening indicated that the assay was reproducible (av Z-factor = 0.80) and
Development and optimization of a useful assay for determining Hsp90's inherent ATPase activity.
Journal:Bioorg Med Chem
PubMed ID:16213144
The Hsp90 molecular chaperone is responsible for the conformational maturation of nascent polypeptides and the rematuration of denatured proteins. Inhibition of Hsp90 represents a promising approach towards the treatment of cancer because numerous signaling cascades can be simultaneously targeted by disruption of the Hsp90-mediated process. Hsp90's ATPase activity is essential
Fly and mammalian lipid phosphate phosphatase isoforms differ in activity both in vitro and in vivo.
Journal:EMBO Rep
PubMed ID:12856002
Wunen (Wun), a homologue of a lipid phosphate phosphatase (LPP), has a crucial function in the migration and survival of primordial germ cells (PGCs) during Drosophila embryogenesis. Past work has indicated that the LPP isoforms may show functional redundancy in certain systems, and that they have broad-range lipid phosphatase activities