AuthorsYamasaki S, Sakata-Sogawa K, Hasegawa A, Suzuki T, Kabu K, Sato E, Kurosaki T, Yamashita S, Tokunaga M, Nishida K, Hirano T
JournalJ Cell Biol
PubMed ID17502426
'Zinc is an essential trace element required for enzymatic activity and for maintaining the conformation of many transcription factors; thus, zinc homeostasis is tightly regulated. Although zinc affects several signaling molecules and may act as a neurotransmitter, it remains unknown whether zinc acts as an intracellular second messenger capable of ... More
Antiviral activity of the zinc ionophores pyrithione and hinokitiol against picornavirus infections.
AuthorsKrenn BM, Gaudernak E, Holzer B, Lanke K, Van Kuppeveld FJ, Seipelt J,
JournalJ Virol
PubMed ID18922875
We have discovered two metal ion binding compounds, pyrithione (PT) and hinokitiol (HK), that efficiently inhibit human rhinovirus, coxsackievirus, and mengovirus multiplication. Early stages of virus infection are unaffected by these compounds. However, the cleavage of the cellular eukaryotic translation initiation factor eIF4GI by the rhinoviral 2A protease was abolished ... More
One-electron oxidation of "photo-Fenton" reagents and inhibition of lipid peroxidation.
AuthorsTobin D, Arvanitidis M, Bisby RH
JournalBiochem Biophys Res Commun
PubMed ID12435402
The "photo-Fenton" reagent, 2-mercaptopyridine N-oxide (MPO), which releases a hydroxyl radical on ultraviolet irradiation, has been found to act as an antioxidant. In the peroxidation of linoleate initiated by a water-soluble azo-initiator, MPO has about one-third the activity of the water-soluble vitamin E analogue Trolox C. In contrast, the oxygen-containing ... More
Astrocytes are more resistant than neurons to the cytotoxic effects of increased [Zn(2+)](i).
AuthorsDineley KE, Scanlon JM, Kress GJ, Stout AK, Reynolds IJ
JournalNeurobiol Dis
PubMed ID10964603
Increased intracellular free Zn(2+) ([Zn(2+)](i)) is toxic to neurons. Glia are more resistant to Zn(2+)-mediated toxicity; however, it is not known if this is because glia are less permeable to Zn(2+) or if glia possess intrinsic mechanisms that serve to buffer or extrude excess [Zn(2+)](i). We used the Zn(2+)-selective ionophore ... More
A reevaluation of neuronal zinc measurements: artifacts associated with high intracellular dye concentration.
AuthorsDineley KE, Malaiyandi LM, Reynolds IJ
JournalMol Pharmacol
PubMed ID12181438
The emergence of zinc as a potent neurotoxin has prompted the development of techniques suitable for the measurement of intracellular free zinc ([Zn(2+)](i)) in cultured cells. Accordingly, a new family of Zn(2+)-sensitive fluorophores has become available. Using ionophore-induced elevations of [Zn(2+)](i) in cultured neurons, we measured [Zn(2+)](i)-induced changes in the ... More