5'-bis-pyrenylated oligonucleotides displaying excimer fluorescence provide sensitive probes of RNA sequence and structure.
AuthorsKostenko E, Dobrikov M, Pyshnyi D, Petyuk V, Komarova N, Vlassov V, Zenkova M
JournalNucleic Acids Res
PubMed ID11522831
Oligonucleotide conjugates bearing two pyrene residues attached to 5'-phosphate through a phosphoramide bond were synthesised. Fluorescence spectra of the conjugates show a peak typical of monomer emission (lambda(max) 382 nm) and a broad emission peak with lambda(max )476 nm, which indicates the excimer formation between the two pyrene residues. Conjugation ... More