Pyrenemethyl laurate, a new fluorescent substrate for continuous kinetic determination of lipase activity.
AuthorsNègre A, Salvayre R, Dagan A, Gatt S
JournalBiochim Biophys Acta
PubMed ID2804074
'A fluorescent acyl derivative of pyrenemethanol, pyrenemethyl laurate, was synthesized and used for the determination of several lipases by a continuous kinetic assay. The influence of the physical parameters of the substrate (pyrenemethyl laurate) and its hydrolysis product (pyrenemethanol), on the fluorescence emission was studied. The hydrolysis of pyrenemethyl laurate ... More
Use of pyrenemethyl laurate for fluorescence-based determination of lipase activity in intact living lymphoblastoid cells and for the diagnosis of acid lipase deficiency.
AuthorsNègre-Salvayre A, Dagan A, Gatt S, Salvayre R
JournalBiochem J
PubMed ID8397511
Pyrenemethyl laurate (PMLes), a fluorogenic substrate for determining in vitro lipase activity [Nègre, Salvayre, Dagan and Gatt (1989) Biochim. Biophys. Acta 1006, 84-88], has been administered to cultured lymphoblastoid cells from normal subjects and from a patient affected with Wolman disease, which is characterized by a deficiency of lysosomal acid ... More