A robust method for production of MHC tetramers with small molecule fluorophores.
AuthorsRamachandiran V, Grigoriev V, Lan L, Ravkov E, Mertens SA, Altman JD
JournalJ Immunol Methods
PubMed ID17187819
Tetramers of major histocompatibility complex molecules (MHC) are now well-established reagents for the detection of antigen-specific T cells by flow cytometry. MHC tetramers are prepared by mixing enzymatically biotinylated MHC molecules with commercial preparations of streptavidin, usually conjugated to a fluorescent phycobiliprotein such as phycoerythrin (PE) or allophycocyanin (APC). While ... More