Double-hexahistidine tag with high-affinity binding for protein immobilization, purification, and detection on ni-nitrilotriacetic acid surfaces.
AuthorsKhan F, He M, Taussig MJ
JournalAnal Chem
PubMed ID16642995
'There is a particular need in protein analysis and purification for specific, functional, and generic methods of protein immobilization on solid supports. Here we describe a double-hexahistidine (His6) tag sequence, comprising two hexahistidines separated by an 11-amino acid spacer, which shows at least 1 order of magnitude stronger binding to ... More
Selective proteome-wide detection of hydrophobic integral membrane proteins using a novel fluorescence-based staining technology.
AuthorsHart C, Schulenberg B, Patton WF
JournalElectrophoresis
PubMed ID15300766
Integral proteins containing two or more alpha-helical membrane-spanning domains are underrepresented in two-dimensional gels. While sodium dodecyl sulfate (SDS)-polyacrylamide gels separate these proteins, staining profiles are usually dominated by high-abundance hydrophilic proteins in the specimen. A fluorescence-based stain is presented that selectively highlights integral proteins containing two or more alpha-helical ... More