pHrodo™ Antibody Labeling Kits

Name: pHrodo™ Antibody Labeling Kits
SKU: P35355
Price: 7851.00 CNY

pHrodo Deep Red 抗体标记试剂盒提供了轻松标记和纯化抗体以进行抗体内化研究所需的材料。胺反应性 pHrodo Deep Red 染料与抗体共价结合，产生仅在酸性环境中发荧光的明亮的可溶性偶联物。pHrodo了解更多信息

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货号	数量	激发/发射	标签或染料
P35355	3 x 100 μg 反应	640/655	pHrodo 深红色
P35356	3 x 1 mg 反应	640/655	pHrodo 深红色
P36020	3 x 100 μg 反应	560/585 nm	pHrodo Red
P36021	3 x 1 mg	560/585 nm	pHrodo Red
P36022	3 x 100 μg 反应	505/525 nm	pHrodo Green
P36023	3 x 1 mg	505/525 nm	pHrodo Green
P36014	1 个试剂盒	560/585	pHrodo Red
P36015	1 个试剂盒	505/525 nm	pHrodo Green

8 选项

货号 P35355

价格（CNY)

7,851.00

Each

添加至购物车

数量:

3 x 100 μg 反应

激发/发射:

640/655

标签或染料:

pHrodo 深红色

价格（CNY)

7,851.00

Each

添加至购物车

pHrodo Deep Red 抗体标记试剂盒提供了轻松标记和纯化抗体以进行抗体内化研究所需的材料。胺反应性 pHrodo Deep Red 染料与抗体共价结合，产生仅在酸性环境中发荧光的明亮的可溶性偶联物。pHrodo Deep Red 标记试剂盒使研究抗体内化比以往更容易，结果确定性更高且优化步骤更少，因为 pHrodo Deep Red 染料仅在晚期内体和溶酶体中发荧光。

pHrodo Deep Red 抗体标记试剂盒特点包括：
•出色的信号窗口—中性 pH 值条件下背景极低，能够实现较大信噪比以提高结果确定性
• 多重— Cy5 通道可进行多色实验
• 低 pKa—可消除来自非内化物质的非特异性信号，并且仅在晚期内体和溶酶体中发荧光
• 便利—标记通常需要 2 小时，手动操作时间少于 30 min

pHrodo Deep Red 是一种低背景 pH 传感器染料，其在中性条件下不显示信号，并且仅在酸性环境中发荧光。这种独特的特性有助于实现快速检测开发，并提高研究抗体内化和内吞/吞噬途径时结果的确定性。pHrodo Deep Red 染料有助于更好地鉴别细胞外内化物质，因为其约 5 pKa，在进入晚期内体和溶酶体之前不会发荧光。pHrodo Deep Red 染料可使用 Cy5 荧光过滤器组进行检测，并经过验证可用于多种应用，包括流式细胞分析、荧光显微镜检测和高内涵分析 (HCA)。

了解更多关于 pHrodo 染料和抗体内化的信息。

了解更多关于抗体标记的信息›

规格

颜色深红色

检测方法荧光

激发/发射640/655

适用于（应用）Flow Cytometry, Fluoresence Microscopy

标记规模100 μg

产品类型抗体标记试剂盒

数量3 x 100 μg 反应

反应一部分TFP 酯

足够用于3 x 100 μg Reactions

化学反应性胺

标记目标抗体, Proteins

标签或染料pHrodo 深红色

溶解度Water, DMSO (Dimethylsulfoxide)

Unit SizeEach

常见问题解答 (FAQ)

May I store antibodies or other proteins labeled with the pHrodo Deep Red Antibody Labeling Kit (Cat. Nos. P35355, P35356) in solutions containing sodium azide?

We do not recommend storing antibodies or other proteins labeled with the pHrodo Deep Red Antibody Labeling Kit (Cat. Nos. P35355, P35356) in solutions containing sodium azide, as pHrodo Deep Red is not stable in the presence of sodium azide. Consider using an alternative, non-azide-based anti-microbial.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do I really need to do a DOL determination when using pHrodo Deep Red Antibody Labeling Kit (Cat. Nos. P35355, P35356)?

A DOL determination is required to determine if a suitable amount of dye is attached to the protein. Without a DOL determination, you would not be able to determine if the protein was adequately labeled. A low fluorescent signal may be the same for a protein that is under-labeled or over-labeled, as over-labeling may cause some level of dye-dye quenching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

May I use the pHrodo Deep Red Antibody Labeling Kit (Cat. Nos. P35355, P35356) to label other proteins that are not antibodies?

Yes. The pHrodo Deep Red Antibody Labeling Kit (Cat. Nos. P35355, P35356) was optimized to label either 100 µg or 1 mg of a ~150 kDa protein (molecular weight of a whole IgG) but can be used to label other proteins. Optimize the mass of protein to label based on the molecular weight of the protein relative to the molecular weight of a whole IgG (~150 kDa).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.