pHrodo™ Deep Red Mammalian and Bacterial Cell Labeling Kit - FAQs

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3 个常见问题解答

May I use other fluorophores with cells labeled with pHrodo Deep Red Mammalian and Bacterial Cell Labeling Kit (Cat. No. P35357)?

Yes. The pHrodo Deep Red Mammalian and Bacterial Cell Labeling Kit (Cat. No. P35357) will label the surface of the cells. You may use other fluorophores to stain or label other organelles in fluorescent colors that do not overlap with the far-red signal of pHrodo Deep Red.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long will the cells remain viable after labeling with the pHrodo Deep Red Mammalian and Bacterial Cell Labeling Kit (Cat. No. P35357)?

How long cells remain viable after labeling with the pHrodo Deep Red Mammalian and Bacterial Cell Labeling Kit (Cat. No. P35357) is unknown. Viability after labeling is dependent upon the inherent properties of the cell and other physiological events that occur while culturing. Assuming the cell type remains viable after labeling, the signal will be halved with each generation.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of reactive chemistry/reactive group is utilized by the pHrodo Deep Red Mammalian and Bacterial Cell Labeling Kit (Cat. No. P35357)?

An amine-reactive pHrodo Deep Red TFP ester is used for the pHrodo Deep Red Mammalian and Bacterial Cell Labeling Kit (Cat. No. P35357). The same reactive pHrodo Deep Red TFP Ester is available as a standalone product (Cat. Nos. P35358, P35359), but they are not of the same amount provided in the kit. The amount of reactive dye provided in the pHrodo Deep Red Mammalian and Bacterial Cell Labeling Kit (Cat. No. P35357) is proprietary.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.