用于检测吞噬作用的 pHrodo™ BioParticles™ 偶联物和用于流式细胞分析的吞噬作用检测试剂盒

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用于检测吞噬作用的 pHrodo™ BioParticles™ 偶联物和用于流式细胞分析的吞噬作用检测试剂盒

引用和文献 (35)

Invitrogen™

用于检测吞噬作用的 pHrodo™ BioParticles™ 偶联物和用于流式细胞分析的吞噬作用检测试剂盒

Name: 用于检测吞噬作用的 pHrodo™ BioParticles™ 偶联物和用于流式细胞分析的吞噬作用检测试剂盒
SKU: P35361
Price: 4837.00 CNY

• 用 pH 敏感性荧光染料进行吞噬和内吞作用的特异性检测 — 分辨内吞颗粒与粘附的细胞外颗粒• 降低敏感实验中的信号变异性并改善时序 — 无需洗涤步骤了解更多信息

更改视图

货号	Color	ProductTypeSpecs	种属
P35361			大肠杆菌
P35360			大肠杆菌
A10025			大肠杆菌
P35365			酿酒酵母

4 选项

货号 P35361

价格（CNY)

4,837.00

飞享价

Ends: 31-Dec-2025

6,556.00

共减 1,719.00 (26%)

Each

添加至购物车

种属:

大肠杆菌

价格（CNY)

4,837.00

飞享价

Ends: 31-Dec-2025

6,556.00

共减 1,719.00 (26%)

Each

添加至购物车

• 用 pH 敏感性荧光染料进行吞噬和内吞作用的特异性检测 — 分辨内吞颗粒与粘附的细胞外颗粒
• 降低敏感实验中的信号变异性并改善时序 — 无需洗涤步骤

仅供科研使用。不可用于人或动物的治疗或诊断。

规格

描述用于检测吞噬作用的 pHrodo™ Red E. coli BioParticles™ 偶联物

染料类型pHrodo™ Red

形式实心

数量5 x 2 mg

运输条件室温

种属大肠杆菌

颜色红色

适用于（应用）细胞分析

适用于（设备）荧光显微镜

产品线BioParticles、pHrodo

产品类型共轭物

Unit SizeEach

内容与储存

在冷冻冰箱（–5° 至 –30°C）中避光储存。

常见问题解答 (FAQ)

I am performing a phagocytosis assay of macrophages engulfing pHrodo-labeled bacteria. What do you recommend for fixation after the phagocytosis?

pHrodo is relatively non-fluorescent until it enters the acidic phagosome, at which point its fluorescence increases. If you fix the sample, the pHrodo will only reflect the pH of the buffer the cells are in, and not the pH of the phagosome. For this reason, we do not recommend fixing samples. If you want to see how many cells engulfed the labeled bacteria, fix the cells and then place the fixed cells in an acidic buffer for the assay.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I store reconstituted pHrodo BioParticles Conjugates for Phagocytosis and Phagocytosis Kit, for Flow Cytometry?

Yes. Once reconstituted, pHrodo BioParticles Conjugates for Phagocytosis and Phagocytosis Kit, for Flow Cytometry (Cat. Nos. P35367, P35361, P35360, P35366, P35364, P35365, A10010) can be stored at 2 - 8 degrees C for several weeks, as long as sodium azide is added to a final concentration of 2 mM. If no sodium azide is added, the cell suspension needs to be used right away or on the same day to avoid contamination. DO NOT FREEZE the resuspended pHrodo bioparticle conjugates.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are the Invitrogen BioParticles products sterile?

While the bacteria have been attenuated with formaldehyde and alcohol desiccation, the BioParticles products are not considered sterile, and we do not recommend incubation of more than 4 hours. This applies to all of our dye-labeled (pHrodo, Alexa Fluor, etc.) and unlabeled BioParticles products.

What is the concentration of bacterial particles for pHrodo BioParticles Conjugates for Phagocytosis?

The bacterial particles per weight for pHrodo BioParticles Conjugates for Phagocytosis, is as follows:

pHrodo E. Coli BioParticles Conjugate for Phagocytosis (Cat. Nos. P35360, P35361, A10025) contains 3 x 10⁸ particles/mg.

pHrodo S. aureus BioParticles Conjugate for Phagocytosis (Cat. Nos. A10010, P35367) contains 3 x 10⁸ particles/mg.

pHrodo Zymosan BioParticles Conjugate for Phagocytosis (Cat. No. P35365) contains 2 x 10⁷ particles/mg.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the type of bond that attaches the dyes to the BioParticles probes?

We use amine-reactive dyes to covalently attach fluorescent dyes to all of our BioParticles probes such as the Escherichia coli (K-12 strain) BioParticles probes, Staphylococcus aureus (Wood strain without protein A) BioParticles, and the Zymosan A (S. cerevisiae) BioParticles probes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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引用和文献 (35)

引用和文献

Abstract

Extracellular Matrix Lumican Promotes Bacterial Phagocytosis, and Lum-/- Mice Show Increased Pseudomonas aeruginosa Lung Infection Severity.

Authors:Shao H, Lee S, Gae-Scott S, Nakata C, Chen S, Hamad AR, Chakravarti S,

Journal:J Biol Chem

PubMed ID:22865855

'Phagocytosis is central to bacterial clearance, but the exact mechanism is incompletely understood. Here, we show a novel and critical role for lumican, the connective tissue extracellular matrix small leucine-rich repeat proteoglycan, in CD14-mediated bacterial phagocytosis. In Psuedomonas aeruginosa lung infections, lumican-deficient (Lum(-/-)) mice failed to clear the bacterium from ... More

SLAM is a microbial sensor that regulates bacterial phagosome functions in macrophages.

Authors:Berger SB, Romero X, Ma C, Wang G, Faubion WA, Liao G, Compeer E, Keszei M, Rameh L, Wang N, Boes M, Regueiro JR, Reinecker HC, Terhorst C,

Journal:Nat Immunol

PubMed ID:20818396

'Phagocytosis is a pivotal process by which macrophages eliminate microorganisms after recognition by pathogen sensors. Here we unexpectedly found that the self ligand and cell surface receptor SLAM functioned not only as a costimulatory molecule but also as a microbial sensor that controlled the killing of gram-negative bacteria by macrophages. ... More

Innate immunity and transcription of MGAT-III and Toll-like receptors in Alzheimer's disease patients are improved by bisdemethoxycurcumin.

Authors:Fiala M, Liu PT, Espinosa-Jeffrey A, Rosenthal MJ, Bernard G, Ringman JM, Sayre J, Zhang L, Zaghi J, Dejbakhsh S, Chiang B, Hui J, Mahanian M, Baghaee A, Hong P, Cashman J

Journal:Proc Natl Acad Sci U S A

PubMed ID:17652175

'We have tested a hypothesis that the natural product curcuminoids, which has epidemiologic and experimental rationale for use in AD, may improve the innate immune system and increase amyloid-beta (Abeta) clearance from the brain of patients with sporadic Alzheimer''s disease (AD). Macrophages of a majority of AD patients do not ... More

Establishment of single-cell screening system for the rapid identification of transcriptional modulators involved in direct cell reprogramming.

Authors:Shin JW, Suzuki T, Ninomiya N, Kishima M, Hasegawa Y, Kubosaki A, Yabukami H, Hayashizaki Y, Suzuki H,

Journal:Nucleic Acids Res

PubMed ID:22879381

'Combinatorial interactions of transcription modulators are critical to regulate cell-specific expression and to drive direct cell reprogramming (e.g. trans-differentiation). However, the identification of key transcription modulators from myriad of candidate genes is laborious and time consuming. To rapidly identify key regulatory factors involved in direct cell reprogramming, we established a ... More

Rooperol as an antioxidant and its role in the innate immune system: An in vitro study.

Authors:Boukes GJ, van de Venter M,

Journal:J Ethnopharmacol

PubMed ID:23085395

'Biologically active rooperol is formed when the glucose subunits of the nontoxic glycoside, hypoxoside, are cleaved by ß-glucosidase. Hypoxoside is isolated from Hypoxis, a medicinal plant genus frequently used by the indigenous people of South Africa as an immune system booster. The aim of this study was to investigate rooperol''s ... More

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