用于检测吞噬作用的 pHrodo™ Red Zymosan BioParticles™ 偶联物
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用于检测吞噬作用的 pHrodo™ Red Zymosan BioParticles™ 偶联物
Invitrogen™

用于检测吞噬作用的 pHrodo™ Red Zymosan BioParticles™ 偶联物

使用用于检测吞噬作用的 pH 敏感性 pHrodo Red Zymosan BioParticles 偶联物,实现更快的染色,得到更准确的结果,且无需洗涤步骤或淬灭染料。此染料-生物颗粒偶联物相较于其他染料-生物颗粒偶联物可得到更快、更精确的结果,可降低敏感实验中的信号变异性并改善时序了解更多信息
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货号数量
P353645 x 1 mg
货号 P35364
价格(CNY)
4,233.00
飞享价
Ends: 31-Dec-2025
5,739.00
共减 1,506.00 (26%)
Each
添加至购物车
数量:
5 x 1 mg
价格(CNY)
4,233.00
飞享价
Ends: 31-Dec-2025
5,739.00
共减 1,506.00 (26%)
Each
添加至购物车
使用用于检测吞噬作用的 pH 敏感性 pHrodo Red Zymosan BioParticles 偶联物,实现更快的染色,得到更准确的结果,且无需洗涤步骤或淬灭染料。此染料-生物颗粒偶联物相较于其他染料-生物颗粒偶联物可得到更快、更精确的结果,可降低敏感实验中的信号变异性并改善时序。pHrodo Red 染料偶联物在细胞外无荧光,但在吞噬体中呈红色强荧光,可专门用于检测细胞内吞噬和内吞作用。pHrodo Red Zymosan BioParticles 偶联物也可与多种蓝色、绿色和远红色染料报告基因(如 GFP、Fuo-4、钙黄绿素、NucBlue、CellEvent Caspase 3/7 green、Mitosox Green 和 Mitotracker Deep Red 等)配合使用进行多通路检测。

pHrodo Red 染料的荧光随 pH 值从中性到酸性降低而显著增强,使其成为研究吞噬作用以及该作用受药物和/或环境因素调节情况的出色工具。它在细胞外无荧光,无需洗涤步骤和淬灭染料。

即用型 pHrodo Red Zymosan BioParticles 偶联物用于成像或流式应用,或 pHrodo Red SE(活化琥珀酰亚胺酯)用于标记您选择的微生物或蛋白。

此外,pHrodo 染料可提供绿色,并可作为大肠杆菌、酵母聚糖、金黄色葡萄球菌和葡聚糖 10,000 MW 的偶联物提供。

仅供科研使用。不可用于人或动物的治疗或诊断。
规格
描述用于检测吞噬作用的 pHrodo™ Red Zymosan BioParticles™ 偶联物
检测方法荧光
染料类型pH 值敏感的染料
激发/发射560/585
数量5 x 1 mg
运输条件室温
种属酿酒酵母
颜色红色
适用于(应用)细胞分析
适用于(设备)Attune™ 声波聚焦流式细胞仪, 共聚焦显微镜, Floid™ 细胞成像系统, 荧光显微镜, 高内涵分析仪, 流式细胞仪
产品线pHrodo™
产品类型染料
Unit SizeEach
内容与储存
在 –20°C 干燥条件下避光储存。

常见问题解答 (FAQ)

Can I store reconstituted pHrodo BioParticles Conjugates for Phagocytosis and Phagocytosis Kit, for Flow Cytometry?

Yes. Once reconstituted, pHrodo BioParticles Conjugates for Phagocytosis and Phagocytosis Kit, for Flow Cytometry (Cat. Nos. P35367, P35361, P35360, P35366, P35364, P35365, A10010) can be stored at 2 - 8 degrees C for several weeks, as long as sodium azide is added to a final concentration of 2 mM. If no sodium azide is added, the cell suspension needs to be used right away or on the same day to avoid contamination. DO NOT FREEZE the resuspended pHrodo bioparticle conjugates.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are the Invitrogen BioParticles products sterile?

While the bacteria have been attenuated with formaldehyde and alcohol desiccation, the BioParticles products are not considered sterile, and we do not recommend incubation of more than 4 hours. This applies to all of our dye-labeled (pHrodo, Alexa Fluor, etc.) and unlabeled BioParticles products.

What is the type of bond that attaches the dyes to the BioParticles probes?

We use amine-reactive dyes to covalently attach fluorescent dyes to all of our BioParticles probes such as the Escherichia coli (K-12 strain) BioParticles probes, Staphylococcus aureus (Wood strain without protein A) BioParticles, and the Zymosan A (S. cerevisiae) BioParticles probes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What can the BioParticles product line be used for?

Fluorescent Bioparticles have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry. We offer E.Coli, S. aureus, and zymosan BioParticles conjugates covalently labeled with a variety of different fluorophores.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.