A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part I: BacMam Labeling and Reagents for Vesicular Structures.
AuthorsDolman NJ, Kilgore JA, Davidson MW,
Journal
PubMed ID23835803
'Fluorescent labeling of vesicular structures in cultured cells, particularly for live cells, can be challenging for a number of reasons. The first challenge is to identify a reagent that will be specific enough where some structures have a number of potential reagents and others very few options. The emergence of ... More
Loss of PiT-1 results in abnormal endocytosis in the yolk sac visceral endoderm.
AuthorsWallingford MC, Giachelli CM
Journal
PubMed ID25138534
'PiT-1 protein is a transmembrane sodium-dependent phosphate (Pi) transporter. PiT-1 knock out (KO) embryos die from largely unknown causes by embryonic day (E) 12.5. We tested the hypothesis that PiT-1 is required for endocytosis in the embryonic yolk sac (YS) visceral endoderm (VE). Here we present data supporting that PiT-1 ... More
Walking nanothermometers: spatiotemporal temperature measurement of transported acidic organelles in single living cells.
AuthorsOyama K, Takabayashi M, Takei Y, Arai S, Takeoka S, Ishiwata S, Suzuki M
JournalLab Chip
PubMed ID22437040
We fabricated fluorescent nanoparticles which monitor temperature changes without sensitivity to pH (4-10) and ionic strength (0-500 mM). The nanothermometers spontaneously enter living HeLa cells via endocytosis, enclosed in acidic organelles, i.e., endosome/lysosome, and then transported along microtubules in a temperature-dependent manner, working as "walking nanothermometers". ... More
A New Role for Myosin II in Vesicle Fission.
AuthorsFlores JA, Balseiro-Gomez S, Cabeza JM, Acosta J, Ramirez-Ponce P, Ales E,
Journal
PubMed ID24959909
An endocytic vesicle is formed from a flat plasma membrane patch by a sequential process of invagination, bud formation and fission. The scission step requires the formation of a tubular membrane neck (the fission pore) that connects the endocytic vesicle with the plasma membrane. Progress in vesicle fission can be ... More
A modern descendant of early green algal phagotrophs.
AuthorsMaruyama S, Kim E,
Journal
PubMed ID23707430
Green algae, land plants, and other photosynthetic eukaryotes possess plastids, such as chloroplasts, which have evolved from cyanobacterial ancestors via endosymbiosis [1]. An early evolutionary merger between heterotrophic eukaryotes and cyanobacteria called primary endosymbiosis gave rise to the first photosynthetic eukaryotes. A series of plastid acquisitions involving engulfment of eukaryotic phototrophs, ... More
Glutathione reductase is essential for host defense against bacterial infection.
AuthorsYan J, Ralston MM, Meng X, Bongiovanni KD, Jones AL, Benndorf R, Nelin LD, Joshua Frazier W, Rogers LK, Smith CV, Liu Y,
JournalFree Radic Biol Med
PubMed ID23623936
Glutathione reductase (Gsr) catalyzes the reduction of glutathione disulfide to glutathione, a major cellular antioxidant. We have recently shown that Gsr is essential for host defense against the gram-negative bacteria Escherichia coli in a mouse model of sepsis. Although we have demonstrated that Gsr is required for sustaining the oxidative ... More
Orbital Single Particle Tracking on a commercial confocal microscope using piezoelectric stage feedback.
AuthorsLanzanò L, Gratton E
Journal
PubMed ID25419461
Single Particle Tracking (SPT) is a technique used to locate fluorescent particles with nanometer precision. In the orbital tracking method the position of a particle is obtained analyzing the distribution of intensity along a circular orbit scanned around the particle. In combination with an active feedback this method allows tracking ... More
Nanotubular crosstalk with distressed cardiomyocytes stimulates the paracrine repair function of mesenchymal stem cells.
AuthorsFigeac F, Lesault PF, Le Coz O, Damy T, Souktani R, Trébeau C, Schmitt A, Ribot J, Mounier R, Guguin A, Manier C, Surenaud M, Hittinger L, Dubois-Randé JL, Rodriguez AM
Journal
PubMed ID24115309
Mesenchymal stem cells (MSC) are known to repair broken heart tissues primarily through a paracrine fashion while emerging evidence indicate that MSC can communicate with cardiomyocytes (CM) through tunneling nanotubes (TNT). Nevertheless, no link has been so far established between these two processes. Here, we addressed whether cell-to-cell communication processes ... More
Improving cytocompatibility of CdTe quantum dots by Schiff-base-coordinated lanthanides surface doping.
AuthorsBuchtelova H, Strmiska V, Skubalova Z, Dostalova S, Michalek P, Krizkova S, Hynek D, Kalina L, Richtera L, Moulick A, Adam V, Heger Z
JournalJ Nanobiotechnology
PubMed ID29673366
'Suitable fluorophores are the core of fluorescence imaging. Among the most exciting, yet controversial, labels are quantum dots (QDs) with their unique optical and chemical properties, but also considerable toxicity. This hinders QDs applicability in living systems. Surface chemistry has a profound impact on biological behavior of QDs. This study ... More
SNAPIN is critical for lysosomal acidification and autophagosome maturation in macrophages.
AuthorsShi B, Huang QQ, Birkett R, Doyle R, Dorfleutner A, Stehlik C, He C, Pope RM
JournalAutophagy
PubMed ID27929705
'We previously observed that SNAPIN, which is an adaptor protein in the SNARE core complex, was highly expressed in rheumatoid arthritis synovial tissue macrophages, but its role in macrophages and autoimmunity is unknown. To identify SNAPIN''s role in these cells, we employed siRNA to silence the expression of SNAPIN in ... More
RabGDIα is a negative regulator of interferon-γ-inducible GTPase-dependent cell-autonomous immunity to Toxoplasma gondii.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID26240314
Phosphoproteomics identifies microglial Siglec-F inflammatory response during neurodegeneration.
Authors
JournalMol Syst Biol
PubMed ID33289969
Differential Clearance of Aβ Species from the Brain by Brain Lymphatic Endothelial Cells in Zebrafish.
Authors
JournalInt J Mol Sci
PubMed ID34769316
A second open reading frame in human enterovirus determines viral replication in intestinal epithelial cells.
Authors
JournalNat Commun
PubMed ID31492846
SARS-CoV-2 S1 Protein Induces Endolysosome Dysfunction and Neuritic Dystrophy.
Authors
JournalFront Cell Neurosci
PubMed ID34776872
Lumenal Galectin-9-Lamp2 interaction regulates lysosome and autophagy to prevent pathogenesis in the intestine and pancreas.
Authors
JournalNat Commun
PubMed ID32855403
Meningeal lymphatic endothelial cells fulfill scavenger endothelial cell function and cooperate with microglia in waste removal from the brain.
Authors
JournalGlia
PubMed ID34487573
SNAPIN is critical for lysosomal acidification and autophagosome maturation in macrophages.
Authors
JournalAutophagy
PubMed ID27929705
eIF4A inactivates TORC1 in response to amino acid starvation.
AuthorsTsokanos FF, Albert MA, Demetriades C, Spirohn K, Boutros M, Teleman AA
JournalEMBO J
PubMed ID26988032
Amino acids regulate TOR complex 1 (TORC1) via two counteracting mechanisms, one activating and one inactivating. The presence of amino acids causes TORC1 recruitment to lysosomes where TORC1 is activated by binding Rheb. How the absence of amino acids inactivates TORC1 is less well understood. Amino acid starvation recruits the ... More
Phasor Analysis of Local ICS Detects Heterogeneity in Size and Number of Intracellular Vesicles.
AuthorsScipioni L, Gratton E, Diaspro A, Lanzanò L
JournalBiophys J
PubMed ID27508445
Organelles represent the scale of organization immediately below that of the cell itself, and their composition, size, and number are tailored to their function. Monitoring the size and number of organelles in live cells is relevant for many applications but can be challenging due to their highly heterogeneous properties. Image ... More
Andrographolide Prevents EV-D68 Replication by Inhibiting the Acidification of Virus-Containing Endocytic Vesicles.
AuthorsWang D, Guo H, Chang J, Wang D, Liu B, Gao P, Wei W
JournalFront Microbiol
PubMed ID30349523
Enterovirus D68 (EV-D68) has emerged as a significant respiratory pathogen that can cause severe respiratory disease and acute neurologic disease. At present, there are no approved antiviral agents or vaccines for EV-D68. In this study, we demonstrate that andrographolide (ADO), an active component of ... More