碘化丙啶 - 1.0 mg/mL 水溶液
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碘化丙啶 - 1.0 mg/mL 水溶液
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碘化丙啶 - 1.0 mg/mL 水溶液

碘化丙啶 (PI) 是一种常用的红色荧光细胞核和染色体复染剂。由于碘化丙啶不能渗透到活细胞中,也通常用于检测群体中的死细胞。PI 通过在碱基之间进行插入而与 DNA 结合,几乎没有序列偏好。在水溶液中,该染料的上限激发/发射波长为 493/636了解更多信息
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货号数量
P356610 mL
货号 P3566
价格(CNY)
982.00
飞享价
Ends: 31-Dec-2025
1,402.00
共减 420.00 (30%)
Each
添加至购物车
数量:
10 mL
价格(CNY)
982.00
飞享价
Ends: 31-Dec-2025
1,402.00
共减 420.00 (30%)
Each
添加至购物车
碘化丙啶 (PI) 是一种常用的红色荧光细胞核和染色体复染剂。由于碘化丙啶不能渗透到活细胞中,也通常用于检测群体中的死细胞。

PI 通过在碱基之间进行插入而与 DNA 结合,几乎没有序列偏好。在水溶液中,该染料的上限激发/发射波长为 493/636 nm。一旦染料被结合,其荧光增强20至30倍,荧光上限激发波长向红色偏移 ∼30–40 nm,荧光上限发射波长向蓝色偏移 ∼15 nm,使得上限激发波长为 535 nm,荧光上限发射波长为 617 nm。

PI 广泛用于荧光显微镜检查、共聚焦激光扫描显微镜、流式细胞分析和荧光测定法。

了解关于碘化丙啶和含碘化丙啶产品的更多信息
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型碘化丙啶
形式溶液
数量10 mL
运输条件室温
子细胞定位细胞质&细胞溶质
发射533⁄617
适用于(应用)活力测定试剂盒
适用于(设备)荧光显微镜, 流式细胞仪
产品类型碘化丙啶
Unit SizeEach
内容与储存
含1瓶碘化丙啶(1.0 mg/mL 水溶液)。
避光储存在冷藏冰箱 (2–8°C) 中。

常见问题解答 (FAQ)

I have a LIVE/DEAD BacLight Bacterial Viability kit that has SYTO 9 and propidium iodide in it. Will I be able to stain eukaryotic cells that have engulfed bacteria and determine if the bacteria are alive or dead using this kit?

Unfortunately, no. SYTO 9 will label the nuclei of live or dead cells, including the eukaryotic cells. Propidium iodide is cell impermeant, and will only enter dead cells. If the eukaryotic cells are dead, they will label with propidium iodide as well. If the eukaryotic cells are alive, propidium iodide will not be able to enter and thus will not label the bacteria inside, whether the bacteria are alive or dead. We are not aware of any way to do a viability assay of bacteria once they have been engulfed by cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is propidium iodide (PI) fixable with glutaraldehyde or paraformaldehyde (PFA)?

PI is not fixable with glutaraldehyde or PFA. Both reagents fix by crosslinking amines. PI and other nucleic acid stains do not inherently bind covalently to nucleic acids and these fixatives do not crosslink the dyes to nucleic acids.
The one fixable nucleic acid stain is Ethidium Monoazide Bromide (EMA), Cat no. E1374); it covalently binds to nucleic acids upon activation by exposure to light.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.