Multipurpose high sensitivity luminescence analyzer (LUANA): use in gel electrophoresis.
AuthorsNeri D, Prospero T, Petrul H, Winter G, Browne M, Vanderpant L
JournalBiotechniques
PubMed ID8800693
Many applications in molecular biology require the rapid and high-sensitivity detection of biological macromolecules. Here we describe a luminescence analyzer (LUANA), featuring xenon lamp-based illumination and a cooled CCD camera as detector. Luminescent samples (gels, membranes or microplates) are placed in a light-tight chamber, and computer software is used to ... More
NF-kappaB inhibits apoptosis in murine mammary epithelia.
AuthorsClarkson RW, Heeley JL, Chapman R, Aillet F, Hay RT, Wyllie A, Watson CJ
JournalJ Biol Chem
PubMed ID10777569
The transcription factor NF-kappaB is a key modulator of apoptosis in a variety of cell types, but to date this specific function of NF-kappaB has not been demonstrated in epithelia. Here, we describe the activation of NF-kappaB during post-lactational involution of the mouse mammary gland, a period of extensive apoptosis ... More
Inhibition of peroxyoxalate chemiluminescence by intercalation of fluorescent acceptors between DNA bases.
AuthorsAlba FJ, Daban JR
JournalPhotochem Photobiol
PubMed ID10212573
We have examined the ability of different fluorescent DNA dyes to become chemically excited by the peroxyoxalate chemiluminescent reaction. The intercalating dyes ethidium bromide and propidium iodide, and the bis-intercalating dyes ethidium homodimer-1, benzoxazolium-4-pyridinium dimer-1 and benzoxazolium-4-quinolinium dimer-1, exhibit an intense chemiluminescence when they are excited by the bis(2,4,6-trichlorophenyl)oxalate (TCPO)-H2O2 ... More
Blockade of maitotoxin-induced endothelial cell lysis by glycine and L-alanine.
AuthorsEstacion M, Weinberg JS, Sinkins WG, Schilling WP
JournalAm J Physiol Cell Physiol
PubMed ID12477666
The maitotoxin (MTX)-induced cell death cascade in bovine aortic endothelial cells (BAECs) is a model for oncotic/necrotic cell death. The cascade is initiated by an increase in cytosolic free Ca(2+) concentration ([Ca(2+)](i)), which is followed by the biphasic uptake of vital dyes. The initial phase of dye entry reflects activation ... More
Actin-dependent mitochondrial motility in mitotic yeast and cell-free systems: identification of a motor activity on the mitochondrial surface.
AuthorsSimon VR, Swayne TC, Pon LA
JournalJ Cell Biol
PubMed ID7615636
Using fluorescent membrane potential sensing dyes to stain budding yeast, mitochondria are resolved as tubular organelles aligned in radial arrays that converge at the bud neck. Time-lapse fluorescence microscopy reveals region-specific, directed mitochondrial movement during polarized yeast cell growth and mitotic cell division. Mitochondria in the central region of the ... More
The Activation of Protein Kinase A by the Calcium-Binding Protein S100A1 Is Independent of Cyclic AMP.
Authors
JournalBiochemistry
PubMed ID28409622
Stem cell heterogeneity drives the parasitic life cycle of Schistosoma mansoni.