Design and characterization of a compact dual channel virus counter.
AuthorsStoffel CL,Kathy RF,Rowlen KL
JournalCytometry. Part A : the journal of the International Society for Analytical Cytology
PubMed ID15830378
Development and evaluation of an automated workstation for single nucleotide polymorphism discrimination using bacterial magnetic particles.
AuthorsTanaka T, Maruyama K, Yoda K, Nemoto E, Udagawa Y, Nakayama H, Takeyama H, Matsunaga T
JournalBiosens Bioelectron
PubMed ID14615090
We designed an automated workstation for magnetic particle-based single nucleotide polymorphism (SNP) discrimination of ALDH genotypes. Bacterial magnetic particles (BMPs) extracted from Magnetospirillum magneticum AMB-1 were used as DNA carriers. The principle for SNP discrimination in this study was based on fluorescence resonance energy transfer (FRET) between FITC (donor) and ... More
Maitotoxin-induced membrane blebbing and cell death in bovine aortic endothelial cells.
AuthorsEstacion M, Schilling WP
JournalBMC Physiol
PubMed ID11231888
'BACKGROUND: Maitotoxin, a potent cytolytic agent, causes an increase in cytosolic free Ca2+ concentration ([Ca2+]i) via activation of Ca2+-permeable, non-selective cation channels (CaNSC). Channel activation is followed by formation of large endogenous pores that allow ethidium and propidium-based vital dyes to enter the cell. Although activation of these cytolytic/oncotic pores, ... More
Maitotoxin activates a nonselective cation channel and a P2Z/P2X(7)-like cytolytic pore in human skin fibroblasts.
AuthorsSchilling WP, Sinkins WG, Estacion M
JournalAm J Physiol
PubMed ID10516106
'Maitotoxin (MTX), a potent cytolytic agent, activates Ca(2+) entry via nonselective cation channels in virtually all types of cells. The identity of the channels involved and the biochemical events leading to cell lysis remain unknown. In the present study, the effect of MTX on plasmalemmal permeability of human skin fibroblasts ... More
Examination of cyanine intercalation dyes for rapid and sensitive detection of DNA fragments by capillary electrophoresis.
AuthorsGibson TJ, Sepaniak MJ
JournalJ Capillary Electrophor
PubMed ID10327373
'Recently, a series of cyanine dyes has been developed that are reported to offer superior fluorescence upon intercalation relative to ethidium bromide (EB). The dimeric cyanine dyes, such as POPO-3 (Molecular Probes, Eugene, OR, U.S.A.), are reported to offer the highest fluorescence enhancement of the cyanine dyes. Although POPO-3 does ... More
Tying a molecular knot with optical tweezers.
AuthorsArai Y, Yasuda R, Akashi K, Harada Y, Miyata H, Kinosita K, Itoh H
JournalNature
PubMed ID10365955
'Filamentous structures are abundant in cells. Relatively rigid filaments, such as microtubules and actin, serve as intracellular scaffolds that support movement and force, and their mechanical properties are crucial to their function in the cell. Some aspects of the behaviour of DNA, meanwhile, depend critically on its flexibility-for example, DNA-binding ... More
Detection of duck hepatitis B virus DNA fragments using on-column intercalating dye labeling with capillary electrophoresis-laser-induced fluorescence.
AuthorsTan WG, Tyrrell DL, Dovichi NJ
JournalJ Chromatogr A
PubMed ID10486738
'A rapid on-column DNA labeling technique is used to detect viral restriction DNA fragments by capillary electrophoresis-laser induced fluorescence detection. Intercalating dyes such as POPO3 or ethidium homodimer-2 are incorporated into the detection buffer. The cationic dyes migrate into the capillary during electrophoresis and bind to the oppositely migrating DNA ... More
Characteristics of different nucleic acid staining dyes for DNA fragment sizing by flow cytometry.
AuthorsYan X, Grace WK, Yoshida TM, Habbersett RC, Velappan N, Jett JH, Keller RA, Marrone BL
JournalAnal Chem
PubMed ID10624155
'An efficient and reliable double-stranded DNA (dsDNA) staining protocol for DNA fragment sizing by flow cytometry is presented. The protocol employs 0.8 microM of PicoGreen to label a wide range of DNA concentrations (0.5 ng/mL to 10,000 ng/mL) without regard to the solution dye/bp ratios and without initial quantification of ... More
Some new properties of DNA-YOYO-3 homodimer complexes revealed by electrophoresis and fluorescence lifetime measurements.
AuthorsPopa LM, Winter S, Löber G
JournalBiochem Mol Biol Int
PubMed ID7696991
The DNA bis-intercalator oxazole homodimer (YOYO-3: 1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-[3-methy l- 2,3-dihydro-(benzo-1,3-oxazole)-2-methylidene]-quinolinium tetraiodide) specifically alters the electrophoretic pattern of covalently closed circular (CCC) DNA molecules. Thus, YOYO-3 seems to remove the CCC DNA supercoils and induces the appearance of additional bands by changing the linking number. It also promotes an unusual "star" activity ... More
Single-nucleotide polymorphism analysis using fluorescence resonance energy transfer between DNA-labeling fluorophore, fluorescein isothiocyanate, and DNA intercalator, POPO-3, on bacterial magnetic particles.
AuthorsNakayama H, Arakaki A, Maruyama K, Takeyama H, Matsunaga T
JournalBiotechnol Bioeng
PubMed ID12910548
To develop an analytical system for single-nucleotide polymorphisms (SNPs), the fluorescence resonance energy transfer (FRET) technique was employed on a bacterial magnetic particle (BMP) surface. A combination of fluorescein isothiocyanate (FITC; excitation 490 nm/emission 520 nm) labeled at the 5' end of DNA and an intercalating compound (POPO-3, excitation 534 ... More
High-throughput single-molecule spectroscopy in free solution.
AuthorsMa Y, Shortreed MR, Yeung ES
JournalAnal Chem
PubMed ID11028623
A high-speed high-throughput single-molecule imaging technique for identifying molecules in free solution based on differences in their fluorescence emission spectra is presented. Unlike previous reports, the entire spectrum, rather than selected wavelengths through optical filters, is recorded. Furthermore, the millisecond data acquisition time means that the molecules do not need ... More
A common reference for cDNA microarray hybridizations.
AuthorsSterrenburg E, Turk R, Boer JM, van Ommen GB, den Dunnen JT
JournalNucleic Acids Res
PubMed ID12409475
Comparisons of expression levels across different cDNA microarray experiments are easier when a common reference is co-hybridized to every microarray. Often this reference consists of one experimental control sample, a pool of cell lines or a mix of all samples to be analyzed. We have developed an alternative common reference ... More
The positioning and dynamics of origins of replication in the budding yeast nucleus.
AuthorsHeun P, Laroche T, Raghuraman MK, Gasser SM
JournalJ Cell Biol
PubMed ID11266454
We have analyzed the subnuclear position of early- and late-firing origins of DNA replication in intact yeast cells using fluorescence in situ hybridization and green fluorescent protein (GFP)-tagged chromosomal domains. In both cases, origin position was determined with respect to the nuclear envelope, as identified by nuclear pore staining or ... More
Maitotoxin and P2Z/P2X(7) purinergic receptor stimulation activate a common cytolytic pore.
AuthorsSchilling WP, Wasylyna T, Dubyak GR, Humphreys BD, Sinkins WG
JournalAm J Physiol
PubMed ID10516107
The effects of maitotoxin (MTX) on plasmalemma permeability are similar to those caused by stimulation of P2Z/P2X(7) ionotropic receptors, suggesting that 1) MTX directly activates P2Z/P2X(7) receptors or 2) MTX and P2Z/P2X(7) receptor stimulation activate a common cytolytic pore. To distinguish between these two possibilities, the effect of MTX was ... More
Impact of Δ9-Tetrahydrocannabinol on Rheumatoid Arthritis Synovial Fibroblasts Alone and in Co-Culture with Peripheral Blood Mononuclear Cells.
Authors
JournalBiomedicines
PubMed ID35625855
Cannabidiol (CBD): a killer for inflammatory rheumatoid arthritis synovial fibroblasts.