Examination of cyanine intercalation dyes for rapid and sensitive detection of DNA fragments by capillary electrophoresis.
AuthorsGibson TJ, Sepaniak MJ
JournalJ Capillary Electrophor
PubMed ID10327373
'Recently, a series of cyanine dyes has been developed that are reported to offer superior fluorescence upon intercalation relative to ethidium bromide (EB). The dimeric cyanine dyes, such as POPO-3 (Molecular Probes, Eugene, OR, U.S.A.), are reported to offer the highest fluorescence enhancement of the cyanine dyes. Although POPO-3 does ... More
Nanogram-level micro-volume DNA assay based on the monomeric cyanine dye PO-PRO-3 iodide.
AuthorsSherwood CS, Haynes CA, Turner RF
JournalBiotechniques
PubMed ID7702839
'Several commercially available DNA-staining dyes can yield highly sensitive fluorimetric assays under optimized conditions. However, the high cost of most dyes, coupled with the need for elaborate or expensive instrumentation and/or mL sample volumes, makes assays of this type very costly for routine use. We present a rapid, highly sensitive ... More