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Premo Autophagy Sensor LC3B-GFP, 1 kit - FAQs

查看更多产品信息 Premo Autophagy Assays with Sensor LC3B, p62, or Tandem Sensor RFP-GFP-LC3B - FAQs (P36235)

2 个常见问题解答

How can I increase the transduction efficiency with the BacMam 2.0 reagents such as the the CellLight and Premo products?

Try varying particle-to-cell ratio (PPC), incubation volume, temperature and, cell density (if adherent cells are transduced). For adherent cells, we recommend a confluence of about 70%. Following the PPC, adjusting the volume is the next best parameter to change to optimize protein expression. If that doesn't work, you can also use the BacMam Enhancer Kit (Cat. No. B10107).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the Premo Autophagy Sensor products to study autophagy in cells in combination with a lentivirus transfection?

Lentivirus transfection may actually induce autophagy, skewing the results. Make certain to include appropriate controls.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.