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Premo™ FUCCI Cell Cycle Sensor (BacMam 2.0) - FAQs

查看更多产品信息 Premo™ FUCCI Cell Cycle Sensor (BacMam 2.0) - FAQs (P36238)

2 个常见问题解答

How can I increase the transduction efficiency with the BacMam 2.0 reagents such as the the CellLight and Premo products?

Try varying particle-to-cell ratio (PPC), incubation volume, temperature and, cell density (if adherent cells are transduced). For adherent cells, we recommend a confluence of about 70%. Following the PPC, adjusting the volume is the next best parameter to change to optimize protein expression. If that doesn't work, you can also use the BacMam Enhancer Kit (Cat. No. B10107).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is there an alternative for the discontinued Premo FUCCI Cell Cycle Sensor (BacMam 2.0) (Cat. Nos. P36237, P36238)?

Unfortunately, our Premo FUCCI Cell Cycle Sensor (BacMam 2.0) (Cat. Nos. P36237, P36238) has been discontinued and there is no alternative product at this time.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.