Search
Citations & References (52)
Invitrogen™
ProLong™ Gold Antifade Mountant
ProLong Gold Antifade is specifically designed to help protect fluorescent dyes (i.e. Alexa Fluor) from fading but is not recommendedRead more
Have Questions?
Change view
| Catalog Number | Quantity |
|---|---|
| P36934 | 5 x 2 mL |
| P10144 | 2 mL |
| P36930 | 10 mL |
Catalog number P36934
Price (CNY)
1,985.00
Online Exclusive
Ends: 31-Dec-2025
2,673.00Save 688.00 (26%)
Each
Quantity:
5 x 2 mL
Price (CNY)
1,985.00
Online Exclusive
Ends: 31-Dec-2025
2,673.00Save 688.00 (26%)
Each
ProLong Gold Antifade is specifically designed to help protect fluorescent dyes (i.e. Alexa Fluor) from fading but is not recommended with fluorescent proteins (GFP, RFP, mCherry). It comes ready-to-use and stored at room temperature—just apply a drop on the sample, add a coverslip, cure, and image. ProLong Gold Antifade Mountant is a hard-setting liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. ProLong Gold delivers optimal protection without significantly quenching the initial fluorescence signal to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments. It is a curing mounting media with a refractive index (RI) of 1.47 that allows longer-term storage of mounted samples. ProLong Gold Antifade Mountant is offered in a ready-to-use 2 mL dropper bottle, or 10 mL bottle and is stored at room temperature. Just apply a drop on the sample, add a coverslip, cure, and image.
Achieve Excellent Refractive Index Matching Without Acrylic Resins
For high-resolution imaging applications, refractive index matching is critical. ProLong Gold Antifade Mountant will cure to an RI of 1.47 and allows you to avoid using traditional acrylic resins (i.e., fingernail polish), which can damage microscope objectives if they make contact. The refractive index determines how much the path of light is bent, or refracted, when entering a material. In microscopy, refractive index differences between specimen and mounting medium should be minimized and can therefore be reduced by using mountants with a higher RI. Optimizing the RI of your experiment will increase the resolution to provide better quality images. Depending on your sample type, thickness, imaging depth and/or staining reagents (e.g. fluorescent proteins or dyes) and whether you need a curing or non-curing mountant, choosing the right mounting media will impact image resolution and should be strongly considered for optimal experimental design.
ProLong Gold Antifade Mountant is not recommended for mounting samples containing fluorescent proteins such as GFP or TagRFP. For superior antifade protection of fluorescent proteins and fluorescent dyes, ProLong Diamond or ProLong Glass Antifade Mountants are recommended. Please note that ProLong Glass mounting media is recommended for samples > 10 microns and all immersion oil imaging applications. For immediate viewing of a sample, choose our non-curing mountant, SlowFade Gold Antifade Mountant.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionProLong™ Gold Antifade Mountant, 5 x 2 mL
Green FeaturesLess hazardous, Sustainable packaging
Quantity5 x 2 mL
Shipping ConditionRoom Temperature
Product LineProLong
Product TypeAntifade Mountant
Reagent TypeAntifade Solution
Volume (Metric)2 mL
Unit SizeEach
Contents & Storage
Storage at room temperature is recommended but can also be stored frozen (-5 to -30°C). Protect from light.
Frequently asked questions (FAQs)
What is the difference between ProLong and SlowFade antifade reagents?
If I use ProLong Gold Antifade Mountant to mount my slides, should I seal the edges?
Some antifade mounting media stay as liquid whereas others harden. What is the benefit of having one that hardens?
I mounted my cells in ProLong antifade mounting medium, but now I want to go back and re-label them. Is there a way I can unmount the coverslip after it has cured (hardened)?
I am using ProLong antifade mounting medium. Do I need to let it cure before imaging? Do I need to seal the edges of the coverslip?
Citations & References (52)
Citations & References
Abstract
Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1.
Journal:J Biol Chem
PubMed ID:16807233
'The molecular nature of store-operated Ca(2+)-selective channels has remained an enigma, due largely to the continued inability to convincingly demonstrate Ca(2+)-selective store-operated currents resulting from exogenous expression of known genes. Recent findings have implicated two proteins, Stim1 and Orai1, as having essential roles in store-operated Ca(2+) entry across the plasma
Human stem cell delivery for treatment of large segmental bone defects.
Journal:Proc Natl Acad Sci U S A
PubMed ID:20133731
'Local or systemic stem cell delivery has the potential to promote repair of a variety of damaged or degenerated tissues. Although various stem cell sources have been investigated for bone repair, few comparative reports exist, and cellular distribution and viability postimplantation remain key issues. In this study, we quantified the
Pseudogout-associated inflammatory calcium pyrophosphate dihydrate microcrystals induce formation of neutrophil extracellular traps.
Journal:
PubMed ID:23677474
'Pseudogout is an autoinflammatory condition triggered by calcium pyrophosphate dehydrate (CPPD) crystal deposition in the joints. The innate immune system is irritated by and responds to the presence of the crystals with an inflammatory response. The synovial fluid contains activated inflammatory macrophages and neutrophil granulocytes. Several details of crystal-induced macrophage
Androgen induces expression of the multidrug resistance protein gene MRP4 in prostate cancer cells.
Journal:Prostate Cancer Prostatic Dis
PubMed ID:17003774
'Multidrug resistance-associated proteins (MRPs) may mediate multidrug resistance in tumor cells. Using a gene array analysis, we have identified MRP4 as an androgen receptor (AR)-regulated gene. Dihydrotestosterone induced MRP4 expression in both androgen-dependent and -independent LNCaP cells, whereas there was little detectable expression in PC-3 or normal prostate epithelial cells.
Estimating relative carbonyl levels in muscle microstructures by fluorescence imaging.
Journal:Anal Bioanal Chem
PubMed ID:18548236
'The increase in the levels of protein carbonyls, biomarkers of oxidative stress, appears to play an important role in aging skeletal muscle. However, the exact distributions of carbonyls among various skeletal muscle microstructures still remain largely unknown, partly owing to the lack of adequate techniques to carry out these measurements.