DAPI和Hoechst 染料非常相似,该如何选用?
DAPI是一种非常普通的蓝色核复染荧光染料,能对固定和通透的细胞和组织的细胞核进行非常明亮的标记。遗憾的是,人们普遍认为它是介于半通透性到非通透性之间的染色剂,对活细胞的染色效果也不一致。Hoechst 33342染料是细胞通透性染料,与DAPI染色有相似的染色结合机制和荧光颜色;它是活细胞成像的首选,且对固定细胞的标记效果和DAPI一样好。
What do ProLong Gold, ProLong Diamond and ProLong Glass antifade mountant reagents contain?
They are composed of proprietary antifades and polymers in aqueous buffers.
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Can I use ProLong Gold, ProLong Diamond, or ProLong Glass mounting medium in a multi-well plate?
No. If the sample cannot be sealed with a coverslip, we recommend the use of SlowFade antifade mountant.
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What can affect sample curing of ProLong Gold-, ProLong Diamond-, and ProLong Glass-mounted samples?
Our ProLong Gold, ProLong Diamond, and ProLong Glass mounting media harden ('cure') by the evaporation of water. Placing the mounted samples in a humid or refrigerated environment would slow down the evaporation process ('curing').
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Are ProLong, ProLong Gold, ProLong Diamond, and ProLong Glass reagents compatible with DyLight dye-conjugated antibodies and streptavidin and with other organic dyes?
We have not tested ProLong, ProLong Gold, ProLong Diamond, or ProLong Glass on DyLight dye-conjugated proteins or with other organic dyes, but it is likely that they will be compatible.
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