Intranasal administration of rotenone in mice attenuated olfactory functions through the lesion of dopaminergic neurons in the olfactory bulb.
AuthorsSasajima H, Miyazono S, Noguchi T, Kashiwayanagi M
Journal
PubMed ID26493152
'Many environmental chemicals are thought to affect brain function. It was reported that chemicals in the nasal cavity directly reach the brain through the connection between olfactory neurons and the olfactory bulb (OB). In this ''olfactory transport,'' xenobiotics absorbed at the nasal mucosa reach the brain by bypassing some physical ... More
Chemically Defined Culture and Cardiomyocyte Differentiation of Human Pluripotent Stem Cells.
AuthorsBurridge PW, Holmström A, Wu JC
Journal
PubMed ID26439715
'Since the first discovery that human pluripotent stem cells (hPS cells) can differentiate to cardiomyocytes, efforts have been made to optimize the conditions under which this process occurs. One of the most effective methodologies to optimize this process is reductionist simplification of the medium formula, which eliminates complex animal-derived components ... More
Actin Cytoskeletal Organization in Drosophila Germline Ring Canals Depends on Kelch Function in a Cullin-RING E3 Ligase.
AuthorsHudson AM, Mannix KM, Cooley L
Journal
PubMed ID26384358
'The Drosophila Kelch protein is required to organize the ovarian ring canal cytoskeleton. Kelch binds and cross-links F-actin in vitro, and it also functions with Cullin 3 (Cul3) as a component of a ubiquitin E3 ligase. How these two activities contribute to cytoskeletal remodeling in vivo is not known. We ... More
SET8 methyltransferase activity during the DNA double-strand break response is required for recruitment of 53BP1.
AuthorsDulev S, Tkach J, Lin S, Batada NN,
Journal
PubMed ID25252681
'DNA double-strand breaks (DSBs) activate a signaling pathway known as the DNA damage response (DDR) which via protein-protein interactions and post-translational modifications recruit signaling proteins, such as 53BP1, to chromatin flanking the lesion. Depletion of the SET8 methyltransferase prevents accumulation of 53BP1 at DSBs; however, this phenotype has been attributed ... More
Targeted exon capture and sequencing in sporadic amyotrophic lateral sclerosis.
'Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease that results in progressive degeneration of motor neurons, ultimately leading to paralysis and death. Approximately 10% of ALS cases are familial, with the remaining 90% of cases being sporadic. Genetic studies in familial cases of ALS have been extremely informative in ... More
Visualizing hypothalamic network dynamics for appetitive and consummatory behaviors.
AuthorsJennings JH, Ung RL, Resendez SL, Stamatakis AM, Taylor JG, Huang J, Veleta K, Kantak PA, Aita M, Shilling-Scrivo K, Ramakrishnan C, Deisseroth K, Otte S, Stuber GD
Journal
PubMed ID25635459
'Optimally orchestrating complex behavioral states, such as the pursuit and consumption of food, is critical for an organism''s survival. The lateral hypothalamus (LH) is a neuroanatomical region essential for appetitive and consummatory behaviors, but whether individual neurons within the LH differentially contribute to these interconnected processes is unknown. Here, we ... More
Rhinovirus Load Is High despite Preserved Interferon-ß Response in Cystic Fibrosis Bronchial Epithelial Cells.
AuthorsDauletbaev N, Das M, Cammisano M, Chen H, Singh S, Kooi C, Leigh R, Beaudoin T, Rousseau S, Lands LC
Journal
PubMed ID26599098
'Lung disease in cystic fibrosis (CF) is often exacerbated following acute upper respiratory tract infections caused by the human rhinovirus (HRV). Pathophysiology of these exacerbations is presently unclear and may involve deficient innate antiviral or exaggerated inflammatory responses in CF airway epithelial cells. Furthermore, responses of CF cells to HRV ... More
Fibroblast growth factor 10 alters the balance between Goblet and Paneth cells in the adult mouse small intestine.
AuthorsAl Alam D, Danopoulos S, Schall K, Sala FG, Almohazey D, Fernandez GE, Georgia S, Frey MR, Ford HR, Grikscheit TC, Bellusci S,
Journal
PubMed ID25721301
Intestinal epithelial cell renewal relies on the right balance of epithelial cell migration, proliferation, differentiation and apoptosis. Intestinal epithelial cells consist of absorptive and secretory lineage. The latter is comprised of goblet, Paneth and enteroendocrine cells. Fibroblast Growth Factor 10 (FGF10) plays a central role in epithelial cell proliferation, survival ... More
The odorant receptor co-receptor from the bed bug, Cimex lectularius L.
AuthorsHansen IA, Rodriguez SD, Drake LL, Price DP, Blakely BN, Hammond JI, Tsujimoto H, Monroy EY, Maio WA, Romero A,
Journal
PubMed ID25411789
Recently, the bed bug, Cimex lectularius L. has re-emerged as a serious and growing problem in many parts of the world. Presence of resistant bed bugs and the difficulty to eliminate them has renewed interest in alternative control tactics. Similar to other haematophagous arthropods, bed bugs rely on their olfactory ... More
A robust pipeline for rapid production of versatile nanobody repertoires.
AuthorsFridy PC, Li Y, Keegan S, Thompson MK, Nudelman I, Scheid JF, Oeffinger M, Nussenzweig MC, Fenyö D, Chait BT, Rout MP
Journal
PubMed ID25362362
Nanobodies are single-domain antibodies derived from the variable regions of Camelidae atypical immunoglobulins. They show promise as high-affinity reagents for research, diagnostics and therapeutics owing to their high specificity, small size (~15 kDa) and straightforward bacterial expression. However, identification of repertoires with sufficiently high affinity has proven time consuming and ... More
MicroRNA-8 targets the Wingless signaling pathway in the female mosquito fat body to regulate reproductive processes.
AuthorsLucas KJ, Roy S, Ha J, Gervaise AL, Kokoza VA, Raikhel AS
Journal
PubMed ID25605933
Female mosquitoes require a blood meal for reproduction, and this blood meal provides the underlying mechanism for the spread of many important vector-borne diseases in humans. A deeper understanding of the molecular mechanisms linked to mosquito blood meal processes and reproductive events is of particular importance for devising innovative vector ... More
Targeting Cellular Squalene Synthase, an Enzyme Essential for Cholesterol Biosynthesis, Is a Potential Antiviral Strategy against Hepatitis C Virus.
AuthorsSaito K, Shirasago Y, Suzuki T, Aizaki H, Hanada K, Wakita T, Nishijima M, Fukasawa M
Journal
PubMed ID25473062
Hepatitis C virus (HCV) exploits host membrane cholesterol and its metabolism for progeny virus production. Here, we examined the impact of targeting cellular squalene synthase (SQS), the first committed enzyme for cholesterol biosynthesis, on HCV production. By using the HCV JFH-1 strain and human hepatoma Huh-7.5.1-derived cells, we found that ... More
Neuronal expression of CB2 cannabinoid receptor mRNAs in the mouse hippocampus.
AuthorsLi Y, Kim J
Journal
PubMed ID26515747
In the brain, CB1 cannabinoid receptors primarily mediate the effects of cannabinoids, but CB2 cannabinoid receptors (CB2Rs) have recently been discovered in the nervous system and also implicated in neuromodulatory roles. To understand the mechanisms of CB2R functions in the brain, it is essential to localize CB2Rs, but the types ... More
Expression of GM-CSF in T Cells Is Increased in Multiple Sclerosis and Suppressed by IFN-ß Therapy.
AuthorsRasouli J, Ciric B, Imitola J, Gonnella P, Hwang D, Mahajan K, Mari ER, Safavi F, Leist TP, Zhang GX, Rostami A
Journal
PubMed ID25917097
Multiple sclerosis (MS) is an autoimmune disease of the CNS. Studies in animal models of MS have shown that GM-CSF produced by T cells is necessary for the development of autoimmune CNS inflammation. This suggests that GM-CSF may have a pathogenic role in MS as well, and a clinical trial ... More
Bulk regional viral injection in neonatal mice enables structural and functional interrogation of defined neuronal populations throughout targeted brain areas.
AuthorsCheetham CE, Grier BD, Belluscio L
Journal
PubMed ID26594154
The ability to label and manipulate specific cell types is central to understanding the structure and function of neuronal circuits. Here, we have developed a simple, affordable strategy for labeling of genetically defined populations of neurons throughout a targeted brain region: Bulk Regional Viral Injection (BReVI). Our strategy involves a ... More
Non-typeable Haemophilus influenzae protects human airway epithelial cells from a subsequent respiratory syncytial virus challenge.
AuthorsHartwig SM, Ketterer M, Apicella MA, Varga SM
JournalVirology
PubMed ID27573069
'Respiratory syncytial virus (RSV) and the common commensal and opportunistic pathogen, non-typeable Haemophilus influenzae (NTHi) both serve as a frequent cause of respiratory infection in children. Although it is well established that some respiratory viruses can increase host susceptibility to secondary bacterial infections, few studies have examined how commensal bacteria ... More
Skeletal Muscle Remodelling as a Function of Disease Progression in Amyotrophic Lateral Sclerosis.
AuthorsJensen L, Jørgensen LH, Bech RD, Frandsen U, Schrøder HD
JournalBiomed Res Int
PubMed ID27195289
'Muscle weakness is considered the pivotal sign of amyotrophic lateral sclerosis (ALS). Knowledge about the skeletal muscle degeneration/regeneration process and the myogenic potential is limited in ALS patients. Therefore, we investigate these processes in a time course perspective by analysing skeletal muscle biopsies from ALS patients collected before and after ... More
C. elegans Methods to Study PTEN.
AuthorsZheng S, Chin-Sang ID
JournalMethods Mol Biol
PubMed ID27033082
'C. elegans encodes a PTEN homolog called DAF-18 and human PTEN can functionally replace DAF-18. Thus C. elegans provides a valuable model organism to study PTEN. This chapter provides methods to study DAF-18/PTEN function in C. elegans. We provide methods to genotype daf-18/Pten mutants, visualize and quantify DAF-18/PTEN in C. ... More
Protein phosphatase PP2A - a novel interacting partner of carnitine transporter OCTN2 (SLC22A5) in rat astrocytes.
AuthorsJuraszek B, Nalecz KA
JournalJ Neurochem
PubMed ID27537937
'l-Carnitine is essential for translocation of fatty acids for their mitochondrial ß-oxidation, a process shown in the brain to take place in astrocytes. Organic cation and carnitine plasma membrane transporter OCTN2 (SLC22A5) is present in astrocytes. OCTN2 activity and localization were previously shown to be regulated by protein kinase C ... More
Localizing Proteins in Fixed Giardia lamblia and Live Cultured Mammalian Cells by Confocal Fluorescence Microscopy.
AuthorsNyindodo-Ogari L, Schwartzbach SD, Skalli O, Estraño CE
JournalMethods Mol Biol
PubMed ID27515076
'Confocal fluorescence microscopy and electron microscopy (EM) are complementary methods for studying the intracellular localization of proteins. Confocal fluorescence microscopy provides a rapid and technically simple method to identify the organelle in which a protein localizes but only EM can identify the suborganellular compartment in which that protein is present. ... More
MicroRNA-7 Regulates the Function of Mitochondrial Permeability Transition Pore by Targeting VDAC1 Expression.
AuthorsChaudhuri AD, Choi DC, Kabaria S, Tran A, Junn E
JournalJ Biol Chem
PubMed ID26801612
'Mitochondrial dysfunction is one of the major contributors to neurodegenerative disorders including Parkinson disease. The mitochondrial permeability transition pore is a protein complex located on the mitochondrial membrane. Under cellular stress, the pore opens, increasing the release of pro-apoptotic proteins, and ultimately resulting in cell death. MicroRNA-7 (miR-7) is a ... More
Infection of human keratinocytes by Streptococcus dysgalactiae subspecies dysgalactiae isolated from milk of the bovine udder.
AuthorsRoma-Rodrigues C, Alves-Barroco C, Raposo LR, Costa MN, Fortunato E, Baptista PV, Fernandes AR, Santos-Sanches I
JournalMicrobes Infect
PubMed ID26655883
'Streptococcus dysgalactiae subsp. dysgalactiae (SDSD) are considered exclusive animal pathogens; however, a putative zoonotic upper limb cellulitis, a prosthetic joint infection and an infective endocarditis were described in humans. To unravel if bovine SDSD isolates are able to infect human cells, the adherence and internalization to human primary keratinocytes of ... More
Antibody Staining in Drosophila Germaria.
AuthorsLie-Jensen A, Haglund K
JournalMethods Mol Biol
PubMed ID27557571
'Drosophila oogenesis is a powerful model for studying a wide spectrum of cellular and developmental processes in vivo. Oogenesis starts in a specialized structure called the germarium, which harbors the stem cells for both germ and somatic cells. The germarium produces egg chambers, each of which will develop into an ... More
A phased strategy to differentiate human CD14+monocytes into classically and alternatively activated macrophages and dendritic cells.
'There are currently several in vitro strategies to differentiate human CD14(+) monocytes isolated from peripheral blood mononuclear cells (PBMCs) into the M1 or M2 macrophage cell types. Each cell type is then verified using flow cytometric analysis of cell-surface markers. Human CD14(+) monocytes have the potential to differentiate into M1 ... More
Effects of local structural transformation of lipid-like compounds on delivery of messenger RNA.
AuthorsLi B, Luo X, Deng B, Giancola JB, McComb DW, Schmittgen TD, Dong Y
JournalSci Rep
PubMed ID26916931
'Lipid-like nanoparticles (LLNs) have shown great potential for RNA delivery. Lipid-like compounds are key components in LLNs. In this study, we investigated the effects of local structural transformation of lipid-like compounds on delivery of messenger RNA. Our results showed that position change of functional groups on lipid-like compounds can dramatically ... More
Dimethyl Sulfoxide Perturbs Cell Cycle Progression and Spindle Organization in Porcine Meiotic Oocytes.
AuthorsLi X, Wang YK, Song ZQ, Du ZQ, Yang CX
JournalPLoS One
PubMed ID27348312
'Meiotic maturation of mammalian oocytes is a precisely orchestrated and complex process. Dimethyl sulfoxide (DMSO), a widely used solvent, drug, and cryoprotectant, is capable of disturbing asymmetric cytokinesis of oocyte meiosis in mice. However, in pigs, DMSO''s effect on oocyte meiosis still remains unknown. We aimed to evaluate if DMSO ... More
Neurotrophin and FGF Signaling Adapter Proteins, FRS2 and FRS3, Regulate Dentate Granule Cell Maturation and Excitatory Synaptogenesis.
AuthorsNandi S, Alviña K, Lituma PJ, Castillo PE, Hébert JM
JournalNeuroscience
PubMed ID29155277
'Dentate granule cells (DGCs) play important roles in cognitive processes. Knowledge about how growth factors such as FGFs and neurotrophins contribute to the maturation and synaptogenesis of DGCs is limited. Here, using brain-specific and germline mouse mutants we show that a module of neurotrophin and FGF signaling, the FGF Receptor ... More
A Low-Cost Pulse Generator for Exacerbating Muscle Fiber Detachment Phenotypes in Zebrafish.
AuthorsThomasi ABD, Sonntag C, Pires DF, Zuidema D, Benci A, Currie PD, Wood AJ
JournalZebrafish
PubMed ID29381425
'Muscle fiber detachment from myoseptal boundaries is a common finding in zebrafish models of muscular dystrophies. In some instances, there is a weakening of the interaction between muscle fiber and myosepta, which is yet to manifest as a fiber detachment phenotype. Therefore, to push the fiber detachment of muscle, mutant ... More
Colorectal cancer liver metastases organoids retain characteristics of original tumor and acquire chemotherapy resistance.
AuthorsBuzzelli JN, Ouaret D, Brown G, Allen PD, Muschel RJ
JournalStem Cell Res
PubMed ID29414601
'Colorectal cancer (CRC) liver metastasis is highly unfavorable for patient outcome and is a leading cause of cancer-related death. Pre-clinical research of CRC liver metastasis predominately utilizes CRC cell lines grown in tissue culture. Here, we demonstrate that CRC liver metastases organoids derived from human specimens recapitulate some aspects of ... More
SHANK1 is differentially expressed during development in CA1 hippocampal neurons and astrocytes.
AuthorsCollins SM, Belagodu AP, Reed SL, Galvez R
JournalDev Neurobiol
PubMed ID29218848
'Recent studies have strongly suggested a role for the synaptic scaffolding protein SHANK1 in normal synaptic structure and signaling. Global SHANK1 knockout (SHANK1-/-) mice demonstrate reduced dendritic spine density, an immature dendritic spine phenotype and impairments in various cognitive tasks. SHANK1 overexpression is associated with increased dendritic spine size and ... More
Regulation of Neurotransmitter Release by Amyloid Precursor Protein Through Synapsin Phosphorylation.
AuthorsLiu A, Zhang Y, Han L, He G, Xie W, Zhou Z, Jia Z
JournalNeurochem Res
PubMed ID29052089
'Abnormal processing of amyloid precursor protein (APP) and aggregation of the Aß peptide are known to play a key role in the pathogenesis of Alzheimer disease, but the function of endogenous APP under normal physiological conditions remains poorly understood. In this study, we investigated presynaptic changes in APP knockout (KO) ... More
Engineering of Systematic Elimination of a Targeted Chromosome in Human Cells.
AuthorsSato H, Kato H, Yamaza H, Masuda K, Nguyen HT, Pham TT, Han X, Hirofuji Y, Nonaka K
JournalBiomed Res Int
PubMed ID28401157
'Embryonic trisomy leads to abortion or congenital genetic disorders in humans. The most common autosomal chromosome abnormalities are trisomy of chromosomes 13, 18, and 21. Although alteration of gene dosage is thought to contribute to disorders caused by extra copies of chromosomes, genes associated with specific disease phenotypes remain unclear. ... More
AuthorsLeppiniemi J, Lahtinen P, Paajanen A, Mahlberg R, Metsä-Kortelainen S, Pinomaa T, Pajari H, Vikholm-Lundin I, Pursula P, Hytönen VP
JournalACS Appl Mater Interfaces
PubMed ID28598154
'We describe herein a nanocellulose-alginate hydrogel suitable for 3D printing. The composition of the hydrogel was optimized based on material characterization methods and 3D printing experiments, and its behavior during the printing process was studied using computational fluid dynamics simulations. The hydrogel was biofunctionalized by the covalent coupling of an ... More
The generation of compartmentalized nanoparticles containing siRNA and cisplatin using a multi-needle electrohydrodynamic strategy.
AuthorsPina MF, Lau W, Scherer K, Parhizkar M, Edirisinghe M, Craig D
JournalNanoscale
PubMed ID28440835
'This study outlines a novel manufacturing technique for the generation of compartmentalized trilayered nanoparticles loaded with an anti-cancer agent and siRNA as a platform for the combination treatment of cancers. More specifically, we describe the use of a multi-needle electrohydrodynamic approach to produce nanoparticles with high size specificity and scalable ... More
Four-Channel Super-Resolution Imaging by 3-D Structured Illumination.
AuthorsEngel U
JournalMethods Mol Biol
PubMed ID28924660
'Multichannel imaging is used as a readout of relative localization of two or more components and is often the first step in investigating functional ensembles in cells. However, the localization volume of diffraction-limited light microscopy (approx. 200 nm by 500 nm) can accommodate hundred of proteins, calling for increased resolution for these ... More
?PNA FRET Pair Miniprobes for Quantitative Fluorescent In Situ Hybridization to Telomeric DNA in Cells and Tissue.
'Measurement of telomere length by fluorescent in situ hybridization is widely used for biomedical and epidemiological research, but there has been relatively little development of the technology in the 20 years since it was first reported. This report describes the use of dual gammaPNA (?PNA) probes that hybridize at alternating ... More
Low-Concentration Tributyltin Decreases GluR2 Expression via Nuclear Respiratory Factor-1 Inhibition.
AuthorsIshida K, Aoki K, Takishita T, Miyara M, Sakamoto S, Sanoh S, Kimura T, Kanda Y, Ohta S, Kotake Y
JournalInt J Mol Sci
PubMed ID28800112
'Tributyltin (TBT), which has been widely used as an antifouling agent in paints, is a common environmental pollutant. Although the toxicity of high-dose TBT has been extensively reported, the effects of low concentrations of TBT are relatively less well studied. We have previously reported that low-concentration TBT decreases a-amino-3-hydroxy-5-methylisoxazole-4-propionic acid ... More
Protective Effects of Oral Astaxanthin Nanopowder against Ultraviolet-Induced Photokeratitis in Mice.
AuthorsHarada F, Morikawa T, Lennikov A, Mukwaya A, Schaupper M, Uehara O, Takai R, Yoshida K, Sato J, Horie Y, Sakaguchi H, Wu CZ, Abiko Y, Lagali N, Kitaichi N
JournalOxid Med Cell Longev
PubMed ID29104724
'Astaxanthin (AST) has a strong antioxidant cellular membrane chaperone protective effect. Recently, a water-soluble nanosized AST (nano-AST) form was produced, which is expected to improve the efficacy of oral intake effects. The purpose of this study was to examine whether oral nano-AST has therapeutic effects on UV-induced photokeratitis in mice.' ... More
Time resolved amplified FRET identifies protein kinase B activation state as a marker for poor prognosis in clear cell renal cell carcinoma.
AuthorsMiles J, Applebee CJ, Leboucher P, Lopez-Fernandez S, Lee DJ, Guarch R, Ward S, Parker PJ, López JI, Larijani B
JournalBBA Clin
PubMed ID29296589
'Clear cell Renal Cell Carcinomas (ccRCC), the largest group of renal tumours, are resistant to classical therapies. The determination of the functional state of actionable biomarkers for the assessment of these adenocarcinomas is essential. The dysregulation of the oncoprotein, PKB/Akt has been linked with poor prognoses in human cancers.' ... More
Impaired Transferrin Receptor Palmitoylation and Recycling in Neurodegeneration with Brain Iron Accumulation.
AuthorsDrecourt A, Babdor J, Dussiot M, Petit F, Goudin N, Garfa-Traoré M, Habarou F, Bole-Feysot C, Nitschké P, Ottolenghi C, Metodiev MD, Serre V, Desguerre I, Boddaert N, Hermine O, Munnich A, Rötig A
JournalAm J Hum Genet
PubMed ID29395073
'Neurodegeneration with brain iron accumulation (NBIA) is a genetically heterogeneous condition characterized by progressive dystonia with iron accumulation in the basal ganglia. How NBIA-associated mutations trigger iron overload remains poorly understood. After studying fibroblast cell lines from subjects carrying both known and unreported biallelic mutations in CRAT and REPS1, we ... More
PD-L1 checkpoint inhibition and anti-CTLA-4 whole tumor cell vaccination counter adaptive immune resistance: A mouse neuroblastoma model that mimics human disease.
AuthorsSrinivasan P, Wu X, Basu M, Rossi C, Sandler AD
JournalPLoS Med
PubMed ID29377881
'Adaptive immune resistance induces an immunosuppressive tumor environment that enables immune evasion. This phenomenon results in tumor escape with progression and metastasis. Programmed cell death-ligand 1 (PD-L1) expressed on tumors is thought to inhibit tumor-infiltrating lymphocytes (TILs) through programmed cell death 1 (PD1), enabling adaptive immune resistance. This study investigates ... More
3D Endothelial Cell Migration.
AuthorsJacobs KA, Gavard J
JournalMethods Mol Biol
PubMed ID29525990
'Endothelial cells have the capacity to shift between states of quiescence and angiogenesis. The early stage of angiogenesis, sprouting, occurs with the synchronized activities of tip cells, which lead the migration of the sprout, and stalk cells, which elongate this vessel sprout. Here, we describe a method to study in ... More
Anisotropic Müller glial scaffolding supports a multiplex lattice mosaic of photoreceptors in zebrafish retina.
AuthorsNagashima M, Hadidjojo J, Barthel LK, Lubensky DK, Raymond PA
JournalNeural Dev
PubMed ID29141686
'The multiplex, lattice mosaic of cone photoreceptors in the adult fish retina is a compelling example of a highly ordered epithelial cell pattern, with single cell width rows and columns of cones and precisely defined neighbor relationships among different cone types. Cellular mechanisms patterning this multiplex mosaic are not understood. ... More
Size-dependent effect of cystine/citric acid-capped confeito-like gold nanoparticles on cellular uptake and photothermal cancer therapy.
AuthorsSaw WS, Ujihara M, Chong WY, Voon SH, Imae T, Kiew LV, Lee HB, Sim KS, Chung LY
JournalColloids Surf B Biointerfaces
PubMed ID29101882
'Physiochemical changes, including size, are known to affect gold nanoparticle cellular internalization and treatment efficacy. Here, we report the effect of four sizes of cystine/citric acid-coated confeito-like gold nanoparticles (confeito-AuNPs) (30, 60, 80 and 100nm) on cellular uptake, intracellular localization and photothermal anticancer treatment efficiency in MDA-MB231 breast cancer cells. ... More
Reprogramming Tumor-Associated Macrophages by Antibody Targeting Inhibits Cancer Progression and Metastasis.
AuthorsGeorgoudaki AM, Prokopec KE, Boura VF, Hellqvist E, Sohn S, Östling J, Dahan R, Harris RA, Rantalainen M, Klevebring D, Sund M, Brage SE, Fuxe J, Rolny C, Li F, Ravetch JV, Karlsson MC
JournalCell Rep
PubMed ID27210762
Tumors are composed of multiple cell types besides the tumor cells themselves, including innate immune cells such as macrophages. Tumor-associated macrophages (TAMs) are a heterogeneous population of myeloid cells present in the tumor microenvironment (TME). Here, they contribute to immunosuppression, enabling the establishment and persistence of solid tumors as well ... More
Development of fluorescence expression tools to study host-mycoplasma interactions and validation in two distant mycoplasma clades.
Fluorescence expression tools for stable and innocuous whole mycoplasma cell labelling have been developed. A Tn4001-derivative mini-transposon affording unmarked, stable mutagenesis in mycoplasmas was modified to allow the constitutive, high-level expression of mCherry, mKO2 and mNeonGreen. These tools were used to introduce the respective fluorescent proteins as chromosomal tags in ... More
Detection of Protein-Protein Interactions and Posttranslational Modifications Using the Proximity Ligation Assay: Application to the Study of the SUMO Pathway.
AuthorsRistic M, Brockly F, Piechaczyk M, Bossis G
JournalMethods Mol Biol
PubMed ID27613043
The detection of protein-protein interactions by imaging techniques often requires the overexpression of the proteins of interest tagged with fluorescent molecules, which can affect their biological properties and, subsequently, flaw experiment interpretations. The recent development of the proximity ligation assays (PLA) technology allows easy visualization of endogenous protein-protein interactions at ... More
Role of Histone Demethylases in Cardiomyocytes Induced to Hypertrophy.
AuthorsRosales W, Carulla J, García J, Vargas D, Lizcano F
JournalBiomed Res Int
PubMed ID27722168
Epigenetic changes induced by histone demethylases play an important role in differentiation and pathological changes in cardiac cells. However, the role of the jumonji family of demethylases in the development of cardiac hypertrophy remains elusive. In this study, the presence of different histone demethylases in cardiac cells was evaluated after ... More
Diverse neurotoxicants target the differentiation of embryonic neural stem cells into neuronal and glial phenotypes.
AuthorsSlotkin TA, Skavicus S, Card J, Levin ED, Seidler FJ
JournalToxicology
PubMed ID27816694
The large number of compounds that needs to be tested for developmental neurotoxicity drives the need to establish in vitro models to evaluate specific neurotoxic endpoints. We used neural stem cells derived from rat neuroepithelium on embryonic day 14 to evaluate the impact of diverse toxicants on their ability to ... More
Rhinosporidium seeberi Nuclear Cycle Activities Using Confocal Microscopy.
AuthorsDelfino D, Mendoza L, Vilela R
JournalJ Parasitol
PubMed ID26461427
Rhinosporidium seeberi is an uncultivated Ichthyosporean infecting animals, including humans. Recent studies suggested R. seeberi undergoes synchronized nuclear division without cytokinesis. We used confocal microscopy to investigate R. seeberi nuclear division cycles in formalin-fixed tissues stained with DAPI and phalloidin. We report that R. seeberi nuclei in juvenile and intermediary ... More
Efficient generation of pancreatic ß-like cells from the mouse gallbladder.
AuthorsWang Y, Galivo F, Pelz C, Haft A, Lee J, Kim SK, Grompe M
JournalStem Cell Res
PubMed ID27833043
Direct reprogramming is a promising approach for the replacement of ß cells in diabetes. Reprogramming of cells originating from the endodermal lineage, such as acinar cells in the pancreas, liver cells and gallbladder cells has been of particular interest because of their developmental proximity to ß cells. Our previous work ... More
Nitric oxide signals are interlinked with calcium signals in normal pancreatic stellate cells upon oxidative stress and inflammation.
The mammalian diffuse stellate cell system comprises retinoid-storing cells capable of remarkable transformations from a quiescent to an activated myofibroblast-like phenotype. Activated pancreatic stellate cells (PSCs) attract attention owing to the pivotal role they play in development of tissue fibrosis in chronic pancreatitis and pancreatic cancer. However, little is known ... More
The Periodontal Pathogen Porphyromonas gingivalis Preferentially Interacts with Oral Epithelial Cells in S Phase of the Cell Cycle.
AuthorsAl-Taweel FB, Douglas CWI, Whawell SA
JournalInfect Immun
PubMed ID27091929
Porphyromonas gingivalis, a key periodontal pathogen, is capable of invading a variety of cells, including oral keratinocytes, by exploiting host cell receptors, including alpha-5 beta-1 (a5ß1) integrin. Previous studies have shown that P. gingivalis accelerates the cell cycle and prevents apoptosis of host cells, but it is not known whether ... More
Lack of Intrinsic GABAergic Connections in the Thalamic Reticular Nucleus of the Mouse.
AuthorsHou G, Smith AG, Zhang ZW
JournalJ Neurosci
PubMed ID27383598
It is generally thought that neurons in the thalamic reticular nucleus (TRN) form GABAergic synapses with other TRN neurons and that these interconnections are important for the function of the TRN. However, the existence of such intrinsic connections is controversial. We combine two complementary approaches to examine intrinsic GABAergic connections ... More
Distribution of a-Gustducin and Vimentin in premature and mature taste buds in chickens.
AuthorsVenkatesan N, Rajapaksha P, Payne J, Goodfellow F, Wang Z, Kawabata F, Tabata S, Stice S, Beckstead R, Liu HX
JournalBiochem Biophys Res Commun
PubMed ID27639649
The sensory organs for taste in chickens (Gallus sp.) are taste buds in the oral epithelium of the palate, base of the oral cavity, and posterior tongue. Although there is not a pan-taste cell marker that labels all chicken taste bud cells, a-Gustducin and Vimentin each label a subpopulation of ... More
Lysosomal protease cathepsin D; a new driver of apoptosis during acute kidney injury.
AuthorsCocchiaro P, Fox C, Tregidgo NW, Howarth R, Wood KM, Situmorang GR, Pavone LM, Sheerin NS, Moles A
JournalSci Rep
PubMed ID27271556
Acute kidney injury (AKI) is an abrupt reduction in kidney function caused by different pathological processes. It is associated with a significant morbidity and mortality in the acute phase and an increased risk of developing End Stage Renal Disease. Despite the progress in the management of the disease, mortality rates ... More
The bvg-repressed gene brtA, encoding biofilm-associated surface adhesin, is expressed during host infection by Bordetella bronchiseptica.
AuthorsNishikawa S, Shinzawa N, Nakamura K, Ishigaki K, Abe H, Horiguchi Y
JournalMicrobiol Immunol
PubMed ID26756546
Bordetella species display phase modulation between Bvg(+) and Bvg(-) phases. Because expression of known virulence factors is up-regulated in the Bvg(+) phase, bacteria in this phase are considered competent for infection. However, the Bvg(-) phase is of negligible importance for infection. No studies have shown that bacterial factors specific to ... More
Pericentric H3K9me3 Formation by HP1 Interaction-defective Histone Methyltransferase Suv39h1.
AuthorsMuramatsu D, Kimura H, Kotoshiba K, Tachibana M, Shinkai Y
JournalCell Struct Funct
PubMed ID27733730
Pericentric regions form epigenetically organized, silent heterochromatin structures that accumulate histone H3 lysine 9 tri-methylation (H3K9me3) and heterochromatin protein 1 (HP1), a methylated H3K9-binding protein. At pericentric regions, Suv39h is the major enzyme that generates H3K9me3. Suv39h also interacts directly with HP1. However, the importance of HP1 interaction for Suv39h-mediated ... More
Cellubrevin/vesicle-associated membrane protein-3-mediated endocytosis and trafficking regulate platelet functions.
Endocytosis is key to fibrinogen (Fg) uptake, trafficking of integrins (aIIbß
CCL11 promotes migration and proliferation of mouse neural progenitor cells.
AuthorsWang F, Baba N, Shen Y, Yamashita T, Tsuru E, Tsuda M, Maeda N, Sagara Y
JournalStem Cell Res Ther
PubMed ID28173860
Neonatal hypoxia-ischemia induces massive brain damage during the perinatal period, resulting in long-term consequences to central nervous system structural and functional maturation. Although neural progenitor cells (NPCs) migrate through the parenchyma and home in to injury sites in the rodent brain, the molecular mechanisms are unknown. We examined the role ... More
Hydroxyapatite as a Vehicle for the Selective Effect of Superparamagnetic Iron Oxide Nanoparticles against Human Glioblastoma Cells.
AuthorsPernal S, Wu VM, Uskokovic V
JournalACS Appl Mater Interfaces
PubMed ID29058880
Despite the early promises of magnetic hyperthermia (MH) as a method for treating cancer, it has been stagnating in the past decade. Some of the reasons for the low effectiveness of superparamagnetic nanoparticles (SPIONs) in MH treatments include (a) low uptake in cancer cells; (b) generation of reactive oxygen species ... More
Exosomes derived from miR-140-5p-overexpressing human synovial mesenchymal stem cells enhance cartilage tissue regeneration and prevent osteoarthritis of the knee in a rat model.
Osteoarthritis (OA) is the most common joint disease throughout the world. Exosomes derived from miR-140-5p-overexpressing synovial mesenchymal stem cells (SMSC-140s) may be effective in treating OA. We hypothesized that exosomes derived from SMSC-140 (SMSC-140-Exos) would enhance the proliferation and migration abilities of articular chondrocytes (ACs) without harming extracellular matrix (ECM) ... More
Amniotic fluid neutrophils can phagocytize bacteria: A mechanism for microbial killing in the amniotic cavity.
AuthorsGomez-Lopez N, Romero R, Garcia-Flores V, Xu Y, Leng Y, Alhousseini A, Hassan SS, Panaitescu B
JournalAm J Reprod Immunol
PubMed ID28703488
Neutrophils are capable of performing phagocytosis, a primary mechanism for microbial killing. Intra-amniotic infection is characterized by an influx of neutrophils into the amniotic cavity. Herein, we investigated whether amniotic fluid neutrophils could phagocytize bacteria found in the amniotic cavity of women with intra-amniotic infection. ... More
Endo-siRNA deficiency results in oocyte maturation failure and apoptosis in porcine oocytes.
AuthorsLiu W, Zhao Q, Piao S, Wang C, Kong Q, An T
JournalReprod Fertil Dev
PubMed ID28399989
Both microRNAs (miRNAs) and endogenous small interfering RNAs (endo-siRNAs) play key regulatory roles in gene expression. Some studies have demonstrated that the function of miRNA is suppressed in mouse oocytes, suggesting that endo-siRNA, not miRNA, is essential for female meiosis. This finding has yet to be confirmed in other species. ... More
Astrocytic expression of the RNA regulator HuR accentuates spinal cord injury in the acute phase.
AuthorsKwan T, Floyd CL, Patel J, Mohaimany-Aponte A, King PH
JournalNeurosci Lett
PubMed ID28487080
We recently showed that the RNA regulator, HuR, is translocated to the cytoplasm in astrocytes in the acute phase of spinal cord injury (SCI), consistent with its activation. HuR positively modulates expression of many pro-inflammatory factors, including IL-1ß, TNF-a, and MMP-12, which are present at high levels in the early ... More
Tight junction protein ZO-1 controls organic cation/carnitine transporter OCTN2 (SLC22A5) in a protein kinase C-dependent way.
AuthorsJurkiewicz D, Michalec K, Skowronek K, Nalecz KA
JournalBiochim Biophys Acta
PubMed ID28257821
OCTN2 (SLC22A5) is an organic cation/carnitine transporter belonging to the solute carrier transporters (SLC) family. OCTN2 is ubiquitously expressed and its presence was shown in various brain cells, including the endothelial cells forming blood-brain barrier, where it was mainly detected at abluminal membrane and in proximity of tight junctions (TJ). ... More
siRNA-Mediated RNA Interference in Precision-Cut Tissue Slices Prepared from Mouse Lung and Kidney.
Small interfering RNA (siRNA)-mediated RNAi interference (RNAi) is a powerful post-transcriptional gene silencing mechanism which can be used to study the function of genes in vitro (cell cultures) and in vivo (animal models). However, there is a translational gap between these models. Hence, there is a need for novel experimental ... More
Dual effect of the wheat Ph1 locus on chromosome synapsis and crossover.
AuthorsMartín AC, Rey MD, Shaw P, Moore G
JournalChromosoma
PubMed ID28365783
Allopolyploids must possess a mechanism for facilitating synapsis and crossover (CO) between homologues, in preference to homoeologues (related chromosomes), to ensure successful meiosis. In hexaploid wheat, the Ph1 locus has a major effect on the control of these processes. Studying a wheat mutant lacking Ph1 provides an opportunity to explore ... More
A novel approach to fabricate dye-encapsulated polymeric micro- and nanoparticles by thin film dewetting technique.
AuthorsChatterjee M, Hens A, Mahato K, Jaiswal N, Mahato N, Nagahanumaiah Chanda N
JournalJ Colloid Interface Sci
PubMed ID28732229
A new method is reported for fabrication of polymeric micro- and nanoparticles from an intermediate patterned surface originated by dewetting of a polymeric thin film. Poly (d, l-lactide-co-glycolide) or PLGA, a biocompatible polymer is used to develop a thin film over a clean glass substrate which dewets spontaneously in the ... More
Ccdc181 is a microtubule-binding protein that interacts with Hook1 in haploid male germ cells and localizes to the sperm tail and motile cilia.
Disruption of murine Hook1 results in a disturbed spermatogenesis and consequently leads to male infertility in mice. Within these mice abnormal sperm development starts with a disorganization of the microtubular manchette in elongating spermatids that leads to an abnormal head shape as well as to distinctive structural changes in the ... More
Heterogeneous distribution of xylan and lignin in tension wood G-layers of the S1+G type in several Japanese hardwoods.
AuthorsHigaki A, Yoshinaga A, Takabe K
JournalTree Physiol
PubMed ID29177443
A gradual shift in the microfibril angle of gelatinous layer (G-layer) of tension wood fibres of the S1+G type has been detected via potassium permanganate (KMnO4) staining. Thus, microfibril angles in fibres of the S1+G type are different from S1+S2+G type fibres. We evaluated the microfibril orientation and presence of ... More
Leishmania donovani chaperonin 10 regulates parasite internalization and intracellular survival in human macrophages.
AuthorsColineau L, Clos J, Moon KM, Foster LJ, Reiner NE
JournalMed Microbiol Immunol
PubMed ID28283754
Protozoa of the genus Leishmania infect macrophages in their mammalian hosts causing a spectrum of diseases known as the leishmaniases. The search for leishmania effectors that support macrophage infection is a focus of significant interest. One such candidate is leishmania chaperonin 10 (CPN10) which is secreted in exosomes and may ... More
Phenotyping Multiple Subsets of Immune Cells In Situ in Formalin-Fixed, Paraffin-Embedded Tissue Sections.
AuthorsMansfield JR, Guest PC, Burks J
JournalAdv Exp Med Biol
PubMed ID28353253
Some somatic illnesses such as peripheral tumours can present with psychiatric symptoms. Many of these are characterized by changes in biomarkers related to the inflammation or immune response. Here, we describe a multispectral imaging protocol that can be used to phenotype immune and other cell types through simultaneous imaging of ... More
Merkel Cell Polyomavirus Small T Antigen Initiates Merkel Cell Carcinoma-like Tumor Development in Mice.
AuthorsVerhaegen ME, Mangelberger D, Harms PW, Eberl M, Wilbert DM, Meireles J, Bichakjian CK, Saunders TL, Wong SY, Dlugosz AA
JournalCancer Res
PubMed ID28512245
Merkel cell carcinoma (MCC) tumor cells express several markers detected in normal Merkel cells, a nonproliferative population of neuroendocrine cells that arise from epidermis. MCCs frequently contain Merkel cell polyomavirus (MCPyV) DNA and express viral transforming antigens, sT and tLT, but the role of these putative oncogenes in MCC development, ... More
Direct and indirect effects of different types of microplastics on freshwater prey (Corbicula fluminea) and their predator (Acipenser transmontanus).
AuthorsRochman CM, Parnis JM, Browne MA, Serrato S, Reiner EJ, Robson M, Young T, Diamond ML, Teh SJ
JournalPLoS One
PubMed ID29108004
We examined whether environmentally relevant concentrations of different types of microplastics, with or without PCBs, directly affect freshwater prey and indirectly affect their predators. Asian clams (Corbicula fluminea) were exposed to environmentally relevant concentrations of polyethylene terephthalate (PET), polyethylene, polyvinylchloride (PVC) or polystyrene with and without polychlorinated biphenyls (PCBs) for ... More
Exposure of spermatozoa to dibutyl phthalate induces abnormal embryonic development in a marine invertebrate Galeolaria caespitosa (Polychaeta: Serpulidae).
AuthorsLu Y, Lin M, Aitken RJ
JournalAquat Toxicol
PubMed ID28843738
In this study, we have investigated the impact of dibutyl phthalate (DBP) on early embryogenesis in a sessile marine invertebrate, Galeolaria caespitosa. DBP was found to induce sperm dysfunction as well as impaired and defective embryogenesis characterised by a particular pattern of abnormality. Thus, after the first cleavage, one blastomere ... More
Stimulus-evoked ERK-dependent phosphorylation of activity-regulated cytoskeleton-associated protein (Arc) regulates its neuronal subcellular localization.
AuthorsNikolaienko O, Eriksen MS, Patil S, Bito H, Bramham CR
JournalNeuroscience
PubMed ID28736134
Activity-regulated cytoskeletal-associated protein (Arc) is implicated as a master regulator of long-term synaptic plasticity and memory formation in mammalian brain. Arc acts at synapses and within the nucleus, but the mechanisms controlling Arc localization and function are little known. As Arc transcription and translation are regulated by extracellularsignal-regulated kinase (ERK) ... More
C-Reactive Protein Binds to Cholesterol Crystals and Co-Localizes with the Terminal Complement Complex in Human Atherosclerotic Plaques.
AuthorsPilely K, Fumagalli S, Rosbjerg A, Genster N, Skjoedt MO, Perego C, Ferrante AMR, De Simoni MG, Garred P
JournalFront Immunol
PubMed ID28900428
Inflammation is a part of the initial process leading to atherosclerosis and cholesterol crystals (CC), found in atherosclerotic plaques, which are known to induce complement activation. The pentraxins C-reactive protein (CRP), long pentraxin 3 (PTX3), and serum amyloid P component (SAP) are serum proteins associated with increased risk of cardiovascular ... More
Fully automated 5-plex fluorescent immunohistochemistry with tyramide signal amplification and same species antibodies.
AuthorsZhang W, Hubbard A, Jones T, Racolta A, Bhaumik S, Cummins N, Zhang L, Garsha K, Ventura F, Lefever MR, Lu Z, Hurley JK, Day WA, Pestic-Dragovich L, Morrison LE, Tang L
JournalLab Invest
PubMed ID28504684
The ability to simultaneously visualize the presence, abundance, location and functional state of many targets in cells and tissues has been described as a true next-generation approach in immunohistochemistry (IHC). A typical requirement for multiplex IHC (mIHC) is the use of different animal species for each primary (1°Ab) and secondary ... More
Cortical inter-hemispheric circuits for multimodal vocal learning in songbirds.
AuthorsPaterson AK, Bottjer SW
JournalJ Comp Neurol
PubMed ID28681379
Vocal learning in songbirds and humans is strongly influenced by social interactions based on sensory inputs from several modalities. Songbird vocal learning is mediated by cortico-basal ganglia circuits that include the SHELL region of lateral magnocellular nucleus of the anterior nidopallium (LMAN), but little is known concerning neural pathways that ... More
Arterial immune protein expression demonstrates the complexity of immune responses in Kawasaki disease arteritis.
AuthorsCameron SA, White SM, Arrollo D, Shulman ST, Rowley AH
JournalClin Exp Immunol
PubMed ID28707750
A more complete understanding of immune-mediated damage to the coronary arteries in children with Kawasaki disease (KD) is required for improvements in patient treatment and outcomes. We recently reported the transcriptional profile of KD coronary arteritis, and in this study sought to determine protein expression of transcriptionally up-regulated immune genes ... More
Investigation of surface topography and stiffness on adhesion and neurites extension of PC12 cells on crosslinked silica aerogel substrates.
AuthorsLynch KJ, Skalli O, Sabri F
JournalPLoS One
PubMed ID29049304
Fundamental understanding and characterization of neural response to substrate topography is essential in the development of next generation biomaterials for nerve repair. Aerogels are a new class of materials with great potential as a biomaterial. In this work, we examine the extension of neurites by PC12 cells plated on matrigel-coated ... More
Efficacy of PD-1 Blockade Is Potentiated by Metformin-Induced Reduction of Tumor Hypoxia.
Blockade of the coinhibitory checkpoint molecule PD-1 has emerged as an effective treatment for many cancers, resulting in remarkable responses. However, despite successes in the clinic, most patients do not respond to PD-1 blockade. Metabolic dysregulation is a common phenotype in cancer, but both patients and tumors are metabolically heterogeneous. ... More
CASAAV: A CRISPR-Based Platform for Rapid Dissection of Gene Function In Vivo.
AuthorsVanDusen NJ, Guo Y, Gu W, Pu WT
JournalCurr Protoc Mol Biol
PubMed ID28967995
In vivo loss-of-function studies are currently limited by the need for appropriate conditional knockout alleles. CRISPR/Cas9 is a powerful tool commonly used to induce loss-of-function mutations in vitro. However, CRISPR components have been difficult to deploy in vivo. To address this problem, we developed the CASAAV (CRISPR/Cas9/AAV-based somatic mutagenesis) platform, ... More
Pax2 is persistently expressed by GABAergic neurons throughout the adult rat dorsal horn.
AuthorsLarsson M
JournalNeurosci Lett
PubMed ID27939388
The transcription factor Pax2 is required for the differentiation of GABAergic neurons in the mouse dorsal horn. Pax2 continues to be expressed in the adult murine spinal cord and has been used as a presumed marker of GABAergic neurons in the superficial dorsal horn of the adult mouse, although a ... More
Neural crest cells utilize primary cilia to regulate ventral forebrain morphogenesis via Hedgehog-dependent regulation of oriented cell division.
AuthorsSchock EN, Brugmann SA
JournalDev Biol
PubMed ID28941984
Development of the brain directly influences the development of the face via both physical growth and Sonic hedgehog (SHH) activity; however, little is known about how neural crest cells (NCCs), the mesenchymal population that comprise the developing facial prominences, influence the development of the brain. We utilized the conditional ciliary ... More
Extracellular vesicles of the blood-brain barrier: Role in the HIV-1 associated amyloid beta pathology.
AuthorsAndrás IE, Leda A, Contreras MG, Bertrand L, Park M, Skowronska M, Toborek M
JournalMol Cell Neurosci
PubMed ID28040512
HIV-infected brains are characterized by increased amyloid beta (Aß) deposition. It is believed that the blood-brain barrier (BBB) is critical for Aß homeostasis and contributes to Aß accumulation in the brain. Extracellular vesicles (ECV), like exosomes, recently gained a lot of attention as potentially playing a significant role in Aß ... More
Behavior of nucleolus in the tobacco male meiocytes involved in cytomixis.
AuthorsMursalimov S, Sidorchuk Y, Deineko E
JournalCell Biol Int
PubMed ID28032378
Behavior of nucleolus during the nuclear migration between plant cells (cytomixis) is studied for the first time in the tobacco male meiosis. As is shown, the nucleolus is located in a nonrandom manner in the migrating nuclei. In the majority of cases, the nucleolus resides on the nuclear pole strictly ... More
Projection targets of medial frontal D1DR-expressing neurons.
AuthorsHan SW, Kim YC, Narayanan NS
JournalNeurosci Lett
PubMed ID28689051
Prefrontal neurons expressing D1-type dopamine receptors (D1DRs) have been implicated in a variety of cognitive processes including working memory and timing. Although D1DRs are most strongly expressed on layer V/VI projection neurons, it is unknown which brain areas are specifically targeted by these projections. Here we selectively marked D1DR neurons ... More
Characterization of luteinizing hormone-releasing hormone receptor type I (LH-RH-I) as a potential molecular target in OCM-1 and OCM-3 human uveal melanoma cell lines.
AuthorsSipos E, Dobos N, Rozsa D, Fodor K, Olah G, Szabo Z, Szekvolgyi L, Schally AV, Halmos G
JournalOnco Targets Ther
PubMed ID29503568
Uveal melanoma (UM) is the most common primary intraocular malignancy with very poor prognosis. Conventional chemotherapy only rarely prolongs the survival, therefore patients require novel treatment modalities. The discovery of specific receptors for hypothalamic hormones on cancer cells has led to the development of radiolabeled and cytotoxic hormone analogs. ... More
Depletion of transglutaminase 2 in neurons alters expression of extracellular matrix and signal transduction genes and compromises cell viability.
AuthorsYunes-Medina L, Paciorkowski A, Nuzbrokh Y, Johnson GVW
JournalMol Cell Neurosci
PubMed ID29197584
The protein transglutaminase 2 (TG2) has been implicated as a modulator of neuronal viability. TG2's role in mediating cell survival processes has been suggested to involve its ability to alter transcriptional events. The goal of this study was to examine the role of TG2 in neuronal survival and to begin ... More
Spontaneous dormancy protects
AuthorsSánchez-Valdéz FJ, Padilla A, Wang W, Orr D, Tarleton RL
JournalElife
PubMed ID29578409
The ability of the Chagas disease agent
K
AuthorsAbiraman K, Tzingounis AV, Lykotrafitis G
JournalFASEB J
PubMed ID29180442
Small conductance calcium-activated potassium (K
Vitamin C-induced epigenomic remodelling in IDH1 mutant acute myeloid leukaemia.
AuthorsMingay M, Chaturvedi A, Bilenky M, Cao Q, Jackson L, Hui T, Moksa M, Heravi-Moussavi A, Humphries RK, Heuser M, Hirst M
JournalLeukemia
PubMed ID28663574
The genomes of myeloid malignancies are characterized by epigenomic abnormalities. Heterozygous, inactivating ten-eleven translocation 2 (TET2) mutations and neomorphic isocitrate dehydrogenase (IDH) mutations are recurrent and mutually exclusive in acute myeloid leukaemia genomes. Ascorbic acid (vitamin C) has been shown to stimulate the catalytic activity of TET2 in vitro and ... More
Suboptimal extracellular pH values alter DNA damage response to induced double-strand breaks.
AuthorsMassonneau J, Ouellet C, Lucien F, Dubois CM, Tyler J, Boissonneault G
JournalFEBS Open Bio
PubMed ID29511618
Conditions leading to unrepaired DNA double-stranded breaks are potent inducers of genetic instability. Systemic conditions may lead to fluctuation of hydrogen ions in the cellular microenvironment, and we show that small variations in extracellular pH, termed suboptimal pHe, can decrease the efficiency of DNA repair in the absence of intracellular ... More
Identifying Quiescent Stem Cells in Hair Follicles.
AuthorsRodriguez CN, Nguyen H
JournalMethods Mol Biol
PubMed ID29030818
Hair follicle stem cells (HFSCs) are noted for their relative quiescence and therefore can be distinguished from other cells by their differential history of cell division. Replicating cells can be labeled by pulsing the animals repeatedly with 5-bromo-2'-deoxyuridine (BrdU) or tritiated thymidine ([ ... More
Detection of oriC-Independent Replication in Escherichia coli Cells.
AuthorsMartel M, Balleydier A, Brochu J, Drolet M
JournalMethods Mol Biol
PubMed ID29177738
In bacteria, replication of the chromosome is normally initiated following the binding of DnaA proteins to the oriC region. However, under certain circumstances, replication can also be initiated independent of the oriC/DnaA system. This is the case, for example, in Escherichia coli cells lacking RNase HI (rnha mutants) or type ... More
Luteoloside prevents lipopolysaccharide-induced osteolysis and suppresses RANKL-induced osteoclastogenesis through attenuating RANKL signaling cascades.
AuthorsSong F, Wei C, Zhou L, Qin A, Yang M, Tickner J, Huang Y, Zhao J, Xu J
JournalJ Cell Physiol
PubMed ID28681916
Bone destruction or osteolysis marked by excessive osteoclastic bone resorption is a very common medical condition. Identification of agents that can effectively suppress excessive osteoclast formation and function is crucial for prevention and treatment of osteolytic conditions such as periprosthetic joint infection and periprosthetic loosening. Luteoloside, a flavonoid, is a ... More