The peroxin pex3p initiates membrane assembly in peroxisome biogenesis.
AuthorsGhaedi K, Tamura S, Okumoto K, Matsuzono Y, Fujiki Y
JournalMol Biol Cell
PubMed ID10848631
'Rat cDNA encoding a 372-amino-acid peroxin was isolated, primarily by functional complementation screening, using a peroxisome-deficient Chinese hamster ovary cell mutant, ZPG208, of complementation group 17. The deduced primary sequence showed approximately 25% amino acid identity with the yeast Pex3p, thereby we termed this cDNA rat PEX3 (RnPEX3). Human and ... More
A fibroblast cell line defective in alkyl-dihydroxyacetone phosphate synthase: a novel defect in plasmalogen biosynthesis.
AuthorsNagan N, Hajra AK, Das AK, Moser HW, Moser A, Lazarow P, Purdue PE, Zoeller RA
JournalProc Natl Acad Sci U S A
PubMed ID9114014
'Using fluorescence-activated cytotoxicity selection, followed by colony autoradiographic screening of the surviving population, we have isolated a unique plasmalogen-deficient Chinese hamster ovary (CHO) cell line. The mutant, NZel-1, showed a dramatic (90%) reduction in the rate of biosynthesis and levels of plasmalogens, as determined using short- and long-term labeling with ... More
Mutants in a macrophage-like cell line are defective in plasmalogen biosynthesis, but contain functional peroxisomes.
AuthorsZoeller RA, Rangaswamy S, Herscovitz H, Rizzo WB, Hajra AK, Das AK, Moser HW, Moser A, Lazarow PB, Santos MJ
JournalJ Biol Chem
PubMed ID1569085
'We have used a fluorescence-activated cytotoxicity protocol, 9-(1''-pyrene)nonanol (P9OH)/UV selection (Morand, O. H., Allen, L.-A. H., Zoeller, R. A., and Raetz, C. R. H. (1990) Biochim. Biophys. Acta 1034, 132-141), to isolate a series of plasmalogen-deficient mutants in a murine, macrophage-like cell line, RAW 264.7. Three of these mutants, RAW.7, ... More
Langmuir monolayer characterization of metal chelating lipids for protein targeting to membranes.
AuthorsPack DW, Arnold FH
JournalChem Phys Lipids
PubMed ID9179995
Targeting and organization of proteins on lipid membranes led to applications in both biological and materials sciences. Coordination of membrane-bound metal ions by surface histidine residues provides a general method for targeting of proteins to membrane surfaces. Here we report the Langmuir monolayer properties of a new class of metal-chelating ... More
A novel pex2 mutant: catalase-deficient but temperature-sensitive PTS1 and PTS2 import.
AuthorsAkiyama N, Ghaedi K, Fujiki Y
JournalBiochem Biophys Res Commun
PubMed ID12054689
We searched for Chinese hamster ovary (CHO) cell mutants defective in peroxisome biogenesis by using peroxisome targeting sequence (PTS) of Pex3p (amino acid residues 1-40)-fused enhanced green fluorescent protein (EGFP). From mutagenized wild-type CHO-K1 cells stably expressing rat Pex2p and Pex3p(1-40)-EGFP, cell colonies resistant to the 9-(1(')-pyrene)nonanol/ultraviolet treatment were examined ... More
A rapid selection for animal cell mutants with defective peroxisomes.
AuthorsMorand OH, Allen LA, Zoeller RA, Raetz CR
JournalBiochim Biophys Acta
PubMed ID2354189
Chinese hamster ovary (CHO) cells take up and incorporate 9-(1'-pyrene)nonanol (P9OH) into phospholipids and neutral lipids. Exposure of P9OH-labeled cells to long wavelength ultraviolet (UV) light causes cell death, because excitation of the pyrene moiety generates reactive oxygen species. CHO mutant cells deficient in plasmalogen biosynthesis and peroxisome assembly (Zoeller, ... More
In situ time-resolved fluorescence spectroscopy in the frequency domain in capillary electrochromatography.
AuthorsHe Y, Geng L
JournalAnal Chem
PubMed ID11985313
In situ time-resolved fluorescence spectroscopy for capillary electrochromatography (CEC) is described in the frequency domain. Fluorescence decay of the solute molecules is collected directly in the packed stationary phase of the CEC capillary. The fluorescence lifetime profile of the solute molecules reveals the microenvironments they experience in the C18 chromatographic ... More
The peroxin Pex14p. cDNA cloning by functional complementation on a Chinese hamster ovary cell mutant, characterization, and functional analysis.
AuthorsShimizu N, Itoh R, Hirono Y, Otera H, Ghaedi K, Tateishi K, Tamura S, Okumoto K, Harano T, Mukai S, Fujiki Y
JournalJ Biol Chem
PubMed ID10212238
Rat cDNA encoding a 376-amino acid peroxin was isolated by functional complementation of a peroxisome-deficient Chinese hamster ovary cell mutant, ZP110, of complementation group 14 (CG14). The primary sequence showed 28 and 24% amino acid identity with the yeast Pex14p from Hansenula polymorpha and Saccharomyces cerevisiae, respectively; therefore, we termed ... More