电穿孔比色皿，0.4 cm，红色
电穿孔比色皿，0.4 cm，红色
Invitrogen™

电穿孔比色皿，0.4 cm，红色

Electroporation uses short, high-voltage electric pulses to overcome the cell membrane and introduce DNA directly into cells. These cuvettes are了解更多信息
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货号数量
P4605050 Cuvettes/Bag
货号 P46050
价格（CNY)
2,376.00
Each
添加至购物车
数量:
50 Cuvettes/Bag
价格（CNY)
2,376.00
Each
添加至购物车

Electroporation uses short, high-voltage electric pulses to overcome the cell membrane and introduce DNA directly into cells. These cuvettes are specifically designed help achieve high DNA transformation efficiency during the introduction of DNA into bacterial, yeast, fungal, and mammalian cells.

Our electroporation cuvettes are precision manufactured from the highest quality materials to ensure optimal and reproducible transformation results.

Electroporation cuvette features
• Compatible with virtually all common electroporation devices
• Gamma-irradiated and individually packaged to ensure sterility
• Color-coded snap cap for easy identification of gap size (red cap for 0.4 cm cuvette)

Note: Electroporation cuvettes are recommended for use with all of our electroporation competent cells.

仅供科研使用。不可用于诊断程序。
规格
直径（公制）0.4 cm
适用于（设备）电穿孔设备
产品类型电穿孔比色皿
数量50 Cuvettes/Bag
运输条件室温
Unit SizeEach
内容与储存
每袋含有 50 个独立包装的无菌比色皿。

常见问题解答 (FAQ)

Will Invitrogen cuvettes fit my electroporator?

Our cuvettes are known as the "Potter-style" cuvettes, and they fit most electroporators that accept a standard size cuvette.

Are Invitrogen electroporator cuvettes autoclavable?

No, one should not autoclave these cuvettes. They are gamma-irradiated to ensure sterility.

What electroporation conditions are recommended for transfection of EcR-293 cells?

5 µg of DNA for 1 x 10e6 cells in a 0.4 cm cuvette. Electroporator settings are routinely 500 µF and 250 Volts but this may have to be optimized depending upon the make and model of the electroporator. Following electroporation, cells are plated out onto 100 mm dishes and media is changed to selective media (400 µg/ml Zeocin) after 24 hours recovery time.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

For mammalian electroporation, do we use carrier DNA? If so, how much and what type?

Carrier DNA is unnecessary for mammalian transfection by electroporation. Invitrogen typically uses 10 µg of target DNA WITHOUT carrier DNA.

Is it possible to electroporate eukaryotic cells in 0.1 cm cuvettes?

With the exception of some fungi, 0.1 cm cuvettes are not used for electroporation of eukaryotic cells. The field strength may be set to an appropriate value but the pulse lengths will typically be too short because of the high conductance of the electroporation medium (due to the short electrode gap distance). The efficiencies of transformation are typically 0.5 - 2 logs lower. Please follow manufacturer's recommended conditions for electroporation.