RNA tertiary folding monitored by fluorescence of covalently attached pyrene.
AuthorsSilverman SK, Cech TR
JournalBiochemistry
PubMed ID10571996
'The pathways by which large RNAs adopt tertiary structure are just beginning to be explored, and new methods that reveal RNA folding are highly desirable. Here we report an assay for RNA tertiary folding in which the fluorescence of a covalently incorporated chromophore is monitored. Folding of the 160-nucleotide Tetrahymena ... More
Synthesis of ODNs containing 4-methylamino-1,8-naphthalimide as a fluorescence probe in DNA.
AuthorsKawai K, Kawabata K, Tojo S, Majima T
JournalBioorg Med Chem Lett
PubMed ID12161134
'Synthesis and fluorescence properties of oligodeoxynucleotides containing 4-methylamino-1,8-naphthalimide (NI) have been described. NI was successfully incorporated into DNA without significant destabilization of DNA whilst retaining its high fluorescence quantum yield. The attachment site of the NI greatly affected its property as an energy acceptor in FRET analysis.' ... More
Photolysis of caged phosphatidic acid induces flagellar excision in Chlamydomonas.
AuthorsGoedhart J, Gadella TW
JournalBiochemistry
PubMed ID15065870
'Phosphatidic (PtdOH) acid formation is recognized as an important step in numerous signaling pathways in both plants and mammals. To study the role of this lipid in signaling pathways, it is of major interest to be able to increase the amount of this lipid directly. Therefore, "caged" PtdOH was synthesized, ... More
Fluorescence assays to study structure, dynamics, and function of RNA and RNA-ligand complexes.